Low temperature efficient corn stalk degradation composite microbial system and application thereof

Abstract

The invention relates to the field of microorganisms, and particularly discloses a low temperature efficient corn stalk degradation composite microbial system and an application thereof. The composite microbial system comprises bacteria and fungi. The bacteria comprise a Cellvibrio bacterial genus, an Azospira bacterial genus, an Arcobacter bacterial genus, an Azonexus bacterial genus, a Clostridium bacterial genus and a Paludibacter bacterial genus. The fungi comprise a Lotharella bacterial genus and a Trichosporon bacterial genus. According to the low temperature efficient corn stalk degradation composite microbial system and the application thereof, the bacterial genera with specific components are used as bacterium source samples, culture media adaptive to the system are adopted, the composite microbial system which is composed of the specific bacterial genera is screened and acclimated through enrichment culture, subculture, enzyme production living bacterium culture and corn stalk degradation culture, corn stalks can be efficiently degraded under a low temperature condition through the system, and the defect that an existing microorganism bacterium agent can only degrade the crop stalks under a medium-high temperature condition is overcome.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a low-temperature and high-efficiency corn stalk-degrading composite bacterial line and its application. Background technique [0002] Straw cellulose is the most abundant and widely sourced organic matter on earth. Returning corn stalks in situ can not only improve the comprehensive utilization rate of corn stalks, but also is an effective technical measure to fertilize and improve soil and improve soil fertility. However, due to the cold and dry climate in northern my country, corn stalks are not easy to decompose in a short period of time, and the direct return to the field will affect the moisture and cultivation. Abandonment and incineration not only cause waste of resources, but also cause environmental pollution. Since the cellulose macromolecules in the straw with a content of 40% to 50% are surrounded by lignin, and the lignin molecules are very stable and not easy to degr...

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Problems solved by technology

[0003] Cellulase in soil plays a very important role in promoting the degradation of straw, but the activity of cellulase in soil is low under natural conditions. By using straw degrading bacteria, the speed of in situ decomposition of straw organic matter is accelerated, which is to promote the in situ degradation of straw. new way of

In order to improve the degradation effect of straw, people have done a lot of research on the application of microbial inoculum in straw returning to the field, and some commercially available microbial inoculants have appeared, but the adaptation temperature of these microbial inoculants is all medium and high temperature (15-50°C), while The climate in the northern region is relatively cold, and medium-high temperature microbial agents cannot adapt, and are prone to problems such as low enzyme activity, poor stability, easy degradation, and poor cold resistance, which cannot benefit the effect of straw returning to the field. Efficient straw-degrading bacteria are of great significance for straw returning to the field

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