Sophorolipid agricultural antibiotic and application thereof to control of fungal diseases of crops

A fungal disease, sophorolipid technology, applied in the field of crop fungal disease prevention and control, can solve the problems of sophorolipid biosurfactant research and development that have not been reported, and achieve the advantages of convenient source of raw materials, simple production process and great application prospects Effect

Inactive Publication Date: 2014-12-03
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research and development of sophorolipid biosur

Method used

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  • Sophorolipid agricultural antibiotic and application thereof to control of fungal diseases of crops
  • Sophorolipid agricultural antibiotic and application thereof to control of fungal diseases of crops
  • Sophorolipid agricultural antibiotic and application thereof to control of fungal diseases of crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: the preparation of sophorolipid agricultural antibiotics, the steps are as follows:

[0038] (1) Take Wicker's yeast (Wickerhamiella domercqiae Y 2A CGMCC NO.3798) was transferred to the slant medium, and cultivated at 30°C for 30 hours to obtain activated Saccharomyces pseudowicka;

[0039] The components of the slant medium are as follows: glucose 20g / L, yeast powder 10g / L, peptone 20g / L, agar powder 20g / L, distilled water to constant volume;

[0040] (2) Inoculate the activated Saccharomyces pseudowicka prepared in step (1) into 50 mL seed culture medium in a 300 mL Erlenmeyer flask, culture at 30° C. and shake at 160 r / min for 24 hours to obtain Saccharomyces pseudowicka seed liquid;

[0041] The seed medium components are as follows: glucose 20g / L, yeast powder 10g / L, peptone 20g / L, distilled water to constant volume;

[0042] (3) Inoculate the seed liquid into the fermentation medium according to the inoculum amount of 5% by volume, and cultivate ...

Embodiment 2

[0046]Embodiment 2: the application of sophorolipid agricultural antibiotics to inhibit pathogenic fungi of crops, the steps are as follows:

[0047] (1) Preparation of pathogenic fungus spore suspension: use inoculation needles to pick a piece of vigorously growing mycelia of various pathogenic crop pathogenic bacteria and inoculate them on fresh PDA medium, and put the flat plate into an incubator for constant temperature cultivation at 28°C; After 10 to 15 days, take out the PDA plate and add an appropriate amount of sterile water, and repeatedly scrape the mycelia with an inoculation needle to release the spores into the sterile water, filter the spores to obtain the spore suspension of the pathogenic bacteria, and dilute the spore concentration to 10 with sterile water. 5 / mL spare;

[0048] (2) Preparation of sophorolipid agricultural antibiotics of different concentrations: get the sophorolipid solid prepared in Example 1, and use distilled water to prepare concentratio...

Embodiment 3

[0051] Embodiment 3: the application of sophorolipid agricultural antibiotics to inhibit pathogenic fungi of crops, the steps are as follows:

[0052] Take 100 μl of 3.0 g / L sophorolipid agricultural antibiotics and 15 mL of PDA solid medium, mix them evenly, pour them onto a plate, inoculate them with 6 mm pieces of pathogenic fungal hyphae after cooling, and simultaneously serve as a negative control. The plates were cultured in a 30°C incubator to observe the growth of mycelium.

[0053] Experimental results such as figure 2 shown by figure 2 It can be seen that some mycelium blocks added with 3.0 g / L sophorolipid agricultural antibiotics grow slowly, and some do not grow, indicating that the sophorolipid agricultural antibiotics have a good inhibitory effect on the growth and spread of mycelia.

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Abstract

The invention relates to a sophorolipid agricultural antibiotic and an application thereof to control of fungal diseases of crops. The sophorolipid agricultural antibiotic is prepared by the following steps: obtaining a biosurfactant sophorolipid by virtue of a microbial fermentation technology; and then preparing a sophorolipid solution by using distilled water, wherein the sophorolipid solution is used as the sophorolipid agricultural antibiotic. The sophorolipid agricultural antibiotic is uniformly sprayed to the blade surfaces of crops or surfaces of fruits to achieve the purpose of preventing and controlling fungal diseases of crops. The sophorolipid agricultural antibiotic provided by the invention that the production process is simple, the raw materials are easy and convenient to obtain, in the cost is low, the output is high, and no toxic effect is caused to a human body. The prepared sophorolipid agricultural antibiotic is simple in dosage form, easy to prepare, remarkable in preventing and control effect and convenient to use and has a huge application prospect.

Description

technical field [0001] The invention relates to a sophorolipid agricultural antibiotic and its application in controlling fungal diseases of crops, belonging to the field of fungal diseases prevention and control of crops. Background technique [0002] my country is a large agricultural producer. According to the data from the National Bureau of Statistics, in 2013, the country’s grain sown area was 112,014 thousand hectares, and the total grain output was 601.94 million tons, accounting for about 17% of the world’s total output, and it is increasing year by year. At the same time, my country's vegetable production has continued to develop steadily, with a planting area of ​​295 million mu and an output of 679 million tons in 2011. At present, my country's population is constantly increasing, and the demand for food and vegetables is on the rise. However, according to statistics, crop diseases and insect pests in my country show a trend of reoccurrence and reoccurrence, wit...

Claims

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Application Information

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IPC IPC(8): C12P19/12A01N43/16A01N43/90A01P3/00C12R1/645
Inventor 陈静伏圣秘
Owner QILU UNIV OF TECH
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