Method for preparing agricultural biostimulant containing fructooligosaccharide from molasses and application of agricultural biostimulant

Abstract

The invention discloses a method for preparing an agricultural biostimulant containing fructooligosaccharide from molasses and application of the agricultural biostimulant, and relates to the technical field of agricultural planting of fruits. The agricultural biostimulant is prepared by fermenting cane molasses with aspergillus oryzae or aspergillus niger, and the specific fermentation method comprises the following steps: inoculating activated strains into a cane molasses diluent with the baume degree of 10-16oBX, and carrying out aerobic fermentation on a shaking table at 25-35 DEG C for 70-72 hours to obtain a strain seed solution; and inoculating the strain seed solution into cane molasses diluent with the baume degree of 20-80 oBX, carrying out aerobic fermentation on a shaking table at 40-55 DEG C for 70-72 hours, and sterilizing to obtain the strain. By controlling the types of fermentation strains, the baume degree of cane molasses, the fermentation temperature and the fermentation time, the obtained fermentation product has the effects of promoting the growth and development of crops and increasing the yield, and is suitable for popularization.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural planting, and in particular to a method for preparing agricultural biostimulants containing fructooligosaccharides by utilizing molasses and application thereof. Background Art

[0002] Sugarcane molasses is the brown-yellow thick liquid left after the sugar industry squeezes out the sugarcane juice and processes such as heating, neutralization, precipitation, filtration, concentration, and crystallization to make sugar. It is commonly known as molasses and has the fragrance of yeast and the sweetness of molasses. The main components of sugarcane molasses are sugars, including sucrose, glucose, and fructose, among which the sucrose content is 45%-48% and the content of other sugars is 12%-24%. Sugarcane molasses also contains protein, amino acids, B vitamins, vitamin C, trace elements, and growth factors, among which the protein content is 3-6% and the amino acid content is 6-9%. Sugarcane molasses is mainly used as an animal feed additive in agriculture. There are also cases where sugarcane molasses is fermented with yeast, fungi, Bacillus, etc. to produce humic acid for crop cultivation, but the cost is high and the available ingredients for crops are small.

[0003] Fructooligosaccharides, also known as fructans and oligofructose, are widely found in plants, but are difficult to extract and difficult to mass produce. Commercial fructooligosaccharide preparations are mainly obtained by using fructosyltransferases in microorganisms and plants to act on sucrose. At present, the application of fructooligosaccharides in agriculture is mainly used as an additive in feed to regulate the balance of microbial flora in the intestines of animals. There are few reports on the application of fructooligosaccharides in crop cultivation. Guangxi is the main sugarcane producing area in the country. Its byproduct, sugarcane molasses, has a high yield and low price. Fructooligosaccharides are fermented to produce sugarcane molasses and used in crop cultivation to expand its application.

[0004] In summary, the object of the present invention is to provide a method for preparing agricultural biostimulants containing fructooligosaccharides using molasses and its application. Summary of the invention

[0005] In view of the above shortcomings, the present invention provides a method for preparing an agricultural biostimulant containing fructooligosaccharides by using molasses and its application. The fermentation method of the present invention can obtain an agricultural biostimulant containing fructooligosaccharides, which can promote the growth and development of crops and increase crop yields, and provides a new application path for sugarcane molasses. The specific technical scheme is as follows:

[0006] A method for preparing an agricultural biostimulant containing fructooligosaccharide by using molasses. The agricultural biostimulant containing fructooligosaccharide is prepared by fermenting sugarcane molasses with Aspergillus oryzae and / or Aspergillus niger.

[0007] Furthermore, the fermentation comprises the following steps:

[0008] (1) preparing a strain seed solution: using a sugarcane molasses dilution as a seed fermentation medium, inoculating the activated strain into the seed fermentation medium, and aerobically fermenting in a shaking incubator at 25-35° C. for 70-72 hours to obtain a strain seed solution;

[0009] (2) Expansion fermentation: using sugarcane molasses diluted liquid as expansion fermentation medium, inoculating the strain seed liquid into the expansion fermentation medium, aerobically fermenting in a shaking incubator at 40-55° C. for 70-72 h, and sterilizing to obtain the product.

[0010] Furthermore, in step (1), the Baume degree of the sugarcane molasses dilution is 10-16° BX.

[0011] Furthermore, in step (1), the inoculation amount of the activated bacteria is 0.2-1% of the weight of the seed fermentation medium.

[0012] Furthermore, in step (2), the Baume degree of the sugarcane molasses dilution is 20-80° BX.

[0013] Furthermore, in step (2), the inoculation amount of the strain seed solution is 0.5-5% of the weight of the expansion fermentation medium.

[0014] The present invention also provides an application of an agricultural biostimulant containing fructooligosaccharide in promoting the growth and development of crops and increasing the yield.

[0015] The present invention also provides a fertilizer containing the agricultural biostimulant containing fructooligosaccharide.

[0016] Furthermore, in the fertilizer, the content of the agricultural biostimulant containing fructooligosaccharide is 50-60%.

[0017] Furthermore, the fertilizer is applied by foliar spraying or root irrigation.

[0018] According to the test, the main components of the agricultural biostimulant containing fructooligosaccharide obtained by fermentation of the method of the present invention are fructooligosaccharide, humic acid, amino acid and other organic acids, among which the total content of fructooligosaccharide is 55%-68%, and the total content of humic acid is about 10%.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The present invention ferments sugarcane molasses by using Aspergillus oryzae and Aspergillus niger, and controls the fermentation conditions, including controlling the Baume degree, fermentation temperature and fermentation time of sugarcane molasses, so that the obtained fermentation product has the effect of promoting the growth and development of crops and increasing the yield. Field tests have found that the agricultural biostimulant containing fructooligosaccharides of the present invention can increase the plant height, plant weight and number of petioles of vegetable crops such as rape and lettuce, and increase the yield per mu by 6-8% compared with conventional fertilizer cultivation. It can also increase the plant height, effective stem number and plant weight of sugarcane, and increase the yield per mu by 2-3 tons / mu compared with conventional fertilizer cultivation, and the sugar content is increased by 1-2 percentage points, and the use effect is remarkable. In addition, the agricultural biostimulant of the present invention has low cost, convenient transportation, and is suitable for promotion. DETAILED DESCRIPTION

[0021] The specific embodiments of the present invention are described in detail below, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments.

[0022] Example 1

[0023] The preparation method of the agricultural biostimulant containing fructooligosaccharide in this embodiment is as follows:

[0024] (1) Preparation of strain seed solution: Sugarcane molasses was diluted to 10° BX as a seed fermentation medium; Aspergillus oryzae S719 (from the Institute of Microbiology, Guangxi Academy of Agricultural Sciences) stored in a -80°C refrigerator was activated to prepare a suspension and then expanded to culture until the number of viable bacteria was 1.0×10 6 cfu / mL, and then inoculate the activated strain into the seed fermentation medium at an inoculation amount of 0.2% of the weight of the seed fermentation medium, 200r / min, aerobic fermentation for 70h, fermentation temperature 25°C, to obtain strain seed liquid;

[0025] (2) Expansion fermentation: dilute sugarcane molasses to 20° BX as an expansion fermentation medium, inoculate the strain seed liquid into the expansion fermentation medium, the inoculation amount is 0.5% of the weight of the expansion fermentation medium, and perform transglycoside fermentation at 200 r / min, aerobic fermentation for 70 hours, and fermentation temperature of 40°C. After fermentation is completed, sterilize and obtain agricultural biostimulant containing fructooligosaccharides.

[0026] After diluting the above-mentioned agricultural biostimulant containing fructooligosaccharide to 6°BX, it was sprayed on the planted Guitang No. 44 tissue culture seedlings. The results of planting showed that the fermentation product could keep the soil sample moist, the survival rate of the tissue culture seedlings planted with sugarcane was as high as 99-100%, the plant height and growth were good, the tissue culture seedlings turned green quickly, and the number of sugarcane tillers was large. However, the soil of the sugarcane tissue culture seedlings cultivated with chemical fertilizers was relatively dry, the survival rate of the tissue culture seedlings was only 55-90%, the growth was poor, the greening speed was slow, and the number of sugarcane tillers was small. It shows that the agricultural biostimulant containing fructooligosaccharide of the present invention can reduce the loss of soil moisture, maintain soil moisture, promote the rooting growth and greening of tissue culture seedlings, and promote the survival and growth of sugarcane tissue culture seedlings.

[0027] Example 2

[0028] The preparation method of the agricultural biostimulant containing fructooligosaccharide in this embodiment is as follows:

[0029] (1) Preparation of strain seed solution: dilute sugarcane molasses to 16° BX as a seed fermentation medium; activate the strain Aspergillus oryzae ZT65 (from Guangxi University, the strain has been preserved in other patents, the preservation number is CCTCC NO: M 2014422) preserved in a -80°C refrigerator to prepare a suspension and then expand the culture until the number of viable bacteria is 1.0×10 6 cfu / mL, and then inoculate the activated strain into the seed fermentation medium at an inoculation amount of 0.5% of the weight of the seed fermentation medium, 200r / min, aerobic fermentation for 72h, fermentation temperature 28°C, to obtain strain seed liquid;

[0030] (2) Expansion fermentation: dilute sugarcane molasses to 60° BX as a fermentation medium, inoculate the strain seed liquid into the fermentation medium, the inoculation amount is 0.5% of the weight of the fermentation medium, and perform transglycoside fermentation at 200 r / min, aerobic fermentation for 72 h, and fermentation temperature of 50° C. After fermentation is completed, sterilize to obtain an agricultural biostimulant containing fructooligosaccharides.

[0031] Application examples:

[0032] The experiment was arranged in April 2020 in the vegetable field of Guo Yunbiao in Yuzhuang Village, Daming Town, Zhongmu County, Henan Province. The test soil is tidal soil with light loam texture, medium to high fertility and uniform fertility. The nutrients of the cultivated layer of this plot are: organic matter 16.2g / kg, total nitrogen 0.99g / kg, available phosphorus (P2O5) 11.3mg / kg, and available potassium (K2O) 89.6mg / kg. The previous crop was spinach with an output of 2100kg per mu. The test crop is rapeseed, and the variety is "Jinpin 535". The "organic water-soluble fertilizer" for testing contains 50% of the agricultural biostimulant containing fructooligosaccharides prepared in this embodiment, which is provided by Guangxi Tingni Biological Products Co., Ltd.

[0033] Experimental method: According to the actual planting situation of farmers, this experiment has three treatments, randomized block arrangement, repeated three times, and the area of ​​the plot is set to 30m 2 .

[0034] Treatment 1: conventional fertilization + 100 mL of the test fertilizer "organic water-soluble fertilizer" diluted with 50 kg of water per mu each time, sprayed once every 5 to 10 days during the rapid growth period of rapeseed, for a total of 2 times;

[0035] Treatment 2: conventional fertilization + spraying the same amount of water as treatment 1;

[0036] Treatment 3: conventional fertilization.

[0037] The experiment was conducted on the basis of local conventional fertilization. Conventional fertilization is: 100kg of commercial organic fertilizer and 20kg of 51% triple compound fertilizer per mu, and 15kg of triple nitro compound fertilizer (28-5-5) during the rapid growth period. The rapeseed in the experimental field was sown on April 12, 2020. According to the requirements of the experimental plan, it was sprayed with fertilizer or water on April 25 and May 2, 2020. On May 9, 2020, 5 plants were randomly selected from each plot for field investigation and seed testing. They were harvested at one time on May 9, and the actual yield was recorded in different areas at the time of harvest. In addition to spraying fertilizer or water as required by the plan, other management measures are the same as those for general rapeseed field production.

[0038] Results and Analysis

[0039] (1) The effects of spraying "organic water-soluble fertilizer" on the biological characteristics of rapeseed are shown in Table 1:

[0040] Table 1 Field survey and seed testing table

[0041] deal with Plant height (cm) Number of blades Single plant weight (g) Leaf Color 1 21.7 11.1 65.0 Dark Green 2 20.1 10.1 61.0 green 3 19.9 10.0 60.7 green

[0042] (Note: The data in the table are the average of three treatments repeated three times)

[0043] Compared with treatment 2 and treatment 3, the plant height of treatment 1 increased by 1.6 cm and 1.8 cm, the number of petioles per plant increased by 1.0 and 1.1, the weight per plant increased by 4.0 g and 4.3 g, and the leaf color was dark green. This shows that on the basis of conventional fertilization, spraying "organic water-soluble fertilizer" can increase the plant height, number of petioles per plant, weight per plant, and dark green leaf color of rapeseed.

[0044] (2) The effect of spraying "organic water-soluble fertilizer" on rapeseed yield is shown in Table 2:

[0045] Table 2 Yield Result Statistics

[0046]

[0047]

[0048] Table 3 Analysis of variance table

[0049] Sources of variation sum of squares Degrees of Freedom variance F <![CDATA[F 0.05 ]]> <![CDATA[F 0.01 ]]> Processing room 171.18 2 85.59 30.46** 6.94 18.00 Repeating 0.28 2 0.14 0.05 6.94 18.00 error 11.22 4 2.81 Total variation 182.68 8

[0050] Table 4 Multiple comparison table

[0051]

[0052] The test results show that: on the basis of local conventional fertilization, 100mL of the test material was diluted with 50kg of water per mu and sprayed once every 5 to 10 days during the rapid growth period of rapeseed, for a total of 2 times. Compared with spraying the same amount of clean water during the same period, it can increase the plant height, number of petioles per plant and weight of rapeseed, and the leaf color is dark green. The average yield per mu increased by 197.9kg, the yield increase rate was 8.7%, and the yield difference in variance analysis was extremely significant.

[0053] Example 3

[0054] The preparation method of the agricultural biostimulant containing fructooligosaccharide in this embodiment is as follows:

[0055] (1) Preparation of strain seed solution: Sugarcane molasses was diluted to 14° BX as a seed fermentation medium; Aspergillus niger FOS-0620 (from South China University of Technology, the strain has been preserved in other patents, and its preservation number is CGMCC No. 6640) preserved in a -80°C refrigerator was activated to prepare a suspension and then expanded to culture until the number of viable bacteria was 1.0×10 5 cfu / mL, and then inoculate the activated strain into the seed fermentation medium at an inoculation amount of 1% of the weight of the seed fermentation medium, 200r / min, aerobic fermentation for 72h, fermentation temperature 35°C, to obtain strain seed liquid;

[0056] (2) Expansion fermentation: 80° BX of sugarcane molasses stock solution is used as the expansion fermentation medium, and the strain seed solution is inoculated into the expansion fermentation medium, with the inoculation amount being 5% of the weight of the expansion fermentation medium, and transglycoside fermentation is carried out at 200 r / min, aerobic fermentation for 72 h, and fermentation temperature of 55°C. After fermentation is completed, sterilization is carried out to obtain agricultural biostimulants containing fructooligosaccharides.

[0057] Application examples:

[0058] Field tests were conducted on lettuce from December 2022 to February 2023. The test fertilizer was provided by Guangxi Tingni Biological Products Co., Ltd. The test fertilizer contained 60% of the agricultural biostimulant containing fructooligosaccharides prepared in this embodiment, and the dosage form was an aqueous solution provided by Guangxi Tingni Biological Products Co., Ltd. The test site was located in the dry land contracted by Shi Huanwu in Jiangzhou Community, Jiangzhou Town, Jiangzhou District, Chongzuo City, Guangxi (107.3962 east longitude, 22.3255 north latitude). The soil type was a yellow mud field with a medium texture, a pH value of 6.3, 26.5g / kg organic matter, 1.18g / kg total nitrogen, 12.4mg / kg available phosphorus, and 88.6mg / kg available potassium. The test plot was flat, with medium fertility and uniformity. The previous crop was Chinese cabbage, with an output of 1060kg per mu and 40kg of compound fertilizer per mu.

[0059] The experiment has 4 treatments, each with 3 replicates, arranged in random blocks, with a plot area of ​​30m 2 .

[0060] Treatment 1: conventional fertilization + test fertilizer; Treatment 2: conventional fertilization + substrate;

[0061] Treatment 3: conventional fertilization + clean water; Treatment 4: conventional fertilization.

[0062] Treatment 4 was carried out according to local fertilization practices, with 40 kg of compound fertilizer (15-15-15) applied per mu as base fertilizer.

[0063] Treatment 1, on the basis of the same fertilization as treatment 4, was fertilized once with the test fertilizer during the crop growth period, with a dosage of 3L per mu and diluted 400 times; Treatment 2, on the basis of the same fertilization as treatment 4, was fertilized with the same amount of test microbial agent matrix at the same time as treatment 1; Treatment 3, on the basis of the same fertilization as treatment 4, was fertilized with the same amount of clean water at the same time as treatment 1. Except for the different fertilization amounts, other agronomic measures and field management were completely the same among the treatments.

[0064] Seeding and seedling raising were carried out on December 9, 2022. Transplanting and planting were carried out on January 3, 2023, and base fertilizer was applied before transplanting and planting. On January 9, the test fertilizer was applied according to the experimental plan. No topdressing was applied during the growth period, and watering was carried out in a timely manner. Except for the different amounts of fertilizer applied, other agronomic measures and field management were completely consistent among the treatments. Mature lettuce was harvested on February 23, the yield of the plot was inspected, and field investigation and seed testing were carried out. 20 representative plants were taken from each plot to investigate plant height and single plant weight.

[0065] Result analysis:

[0066] (1) The effects of different treatments on agronomic traits of lettuce are shown in Table 5:

[0067] Table 5 Field survey and seed testing statistics

[0068] deal with Plant height (cm) Single plant weight (g) 1 21.1 620.2 2 19.7 580.9 3 19.6 580.4 4 19.6 578.7

[0069] As can be seen from Table 1, on the basis of conventional fertilization, the application of the tested fertilizers can increase the plant height and plant weight of lettuce. Compared with treatment 2, treatment 3, and treatment 4, the plant height of treatment 1 increased by 1.4 cm, 1.5 cm, and 1.5 cm, respectively, and the plant weight increased by 39.3 g, 39.8 g, and 41.5 g, respectively.

[0070] (2) The effects of different treatments on yield are shown in Tables 6, 7, and 8:

[0071] Table 6 Yield statistics of different treatment plots

[0072]

[0073] As can be seen from Table 2, treatment 1 increased the average yield per mu by 135.5 kg compared with treatment 2, with an increase rate of 6.47%; compared with treatment 3, the average yield per mu increased by 137.8 kg, with a yield rate of 6.58%; compared with treatment 4, the average yield per mu increased by 142.2 kg, with an increase rate of 6.81%.

[0074] Table 7 Variance analysis results

[0075] Sources of variation sum of squares Degrees of Freedom Mean Square F <![CDATA[F 0.05 ]]> <![CDATA[F 0.01 ]]> Between blocks 0.49 2 0.24 0.78 5.14 10.92 Processing room 87.56 3 29.19 93.40 4.76 9.78 error 1.88 6 0.31 Total variation 89.92 11 8.17

[0076] Table 8 Multiple comparison results

[0077]

[0078] The test results show that, on the basis of conventional fertilization, the application of the test fertilizer can increase the plant height and single plant weight of lettuce, with an average yield of 2231.2 kg per mu. Compared with the application of microbial agent matrix, the average yield increased by 135.5 kg per mu, the yield increase rate was 6.47%, and the yield increase effect was extremely significant; compared with conventional fertilization, the average yield increased by 142.2 kg per mu, the yield increase rate was 6.81%, and the yield increase effect was extremely significant.

[0079] Example 4

[0080] The preparation method of the agricultural biostimulant containing fructooligosaccharide in this embodiment is as follows:

[0081] (1) Preparation of strain seed solution: Sugarcane molasses was diluted to 16° BX as a seed fermentation medium; Aspergillus niger FOS-0620 (from South China University of Technology, the strain has been preserved in other patents, and its preservation number is CGMCC No. 6640) preserved in a -80°C refrigerator was activated to prepare a suspension and then expanded to culture until the number of viable bacteria was 1.0×10 5cfu / mL, and then inoculate the activated strain into the seed fermentation medium at an inoculation amount of 0.5% of the weight of the seed fermentation medium, 200r / min, aerobic fermentation for 72h, fermentation temperature 28°C, to obtain strain seed liquid;

[0082] (2) Expansion fermentation: dilute sugarcane molasses to 60° BX as an expansion fermentation medium, inoculate the strain seed liquid into the expansion fermentation medium, the inoculation amount is 3% of the weight of the expansion fermentation medium, and perform transglycoside fermentation at 200 r / min, aerobic fermentation for 72 hours, and fermentation temperature of 50° C. After fermentation is completed, sterilize to obtain an agricultural biostimulant containing fructooligosaccharides.

[0083] The above-mentioned agricultural biostimulant containing fructooligosaccharides was diluted to 8°BX, sprayed on the seed stems of Guitang No. 44 sugarcane, and sugarcane sugar cane was produced. The results of the planting showed that the sugarcane grew well, the sugarcane leaves were wide, the color was dark green, the sugarcane tillers were good, the number of effective stems was significant, and the average height of the sugarcane plants was 3.6 meters. Compared with the cultivation using chemical fertilizers, the sugarcane grew weakly, the sugarcane leaves were narrow, the color was lighter, the sugarcane tillers were less, the number of effective stems was insufficient, and the average height of the sugarcane plants was 3.0 meters. The fermentation product of the present invention was used to cultivate sugarcane Guitang No. 44, and its yield was 2-3 tons / mu higher than that of chemical fertilizer cultivation, and the sugar content was 1-2 percentage points higher than that of chemical fertilizer cultivation. It is shown that the fermentation product of the present invention is sprayed on the planting of sugarcane sugar cane, which can promote sugarcane tillering, promote sugarcane growth, increase chlorophyll content, promote sugarcane growth, increase sugarcane yield and increase sugar content.

[0084] Comparative Example 1

[0085] In step (1) of this comparative example, the strain seed solution is prepared by diluting sugarcane molasses to 20° BX, and the other steps are the same as those in Example 2;

[0086] Comparative Example 2

[0087] In step (1) of this comparative example, the fermentation temperature is 40°C, and the other steps are the same as those in Example 2;

[0088] Comparative Example 3

[0089] In step (2) of this comparative example, the fermentation temperature is 28°C, and the other steps are the same as those in Example 2;

[0090] Comparative Example 4

[0091] In this comparative example, white sugar was used instead of sugarcane molasses for fermentation, and other fermentation methods were the same as those in Example 2.

[0092] Comparative Example 5

[0093] The fermentation method of sugarcane molasses in this comparative example is as follows: using brewer's yeast (the number of viable bacteria is 1.0×1010 cfu / mL), Bacillus megaterium (viable count 1.0×10 8 cfu / mL) was used to ferment 60°BX sugarcane molasses, the amount of the two bacterial liquids was 5% of the weight of the fermentation liquid, the fermentation temperature was 40°C, and the fermentation was carried out for 96h.

[0094] The fermentation products obtained in Example 2 and the comparative examples were respectively used to conduct field comparative tests:

[0095] The experiment was arranged in April 2020 in Guo Yunbiao's vegetable field in Yuzhuang Village, Dameng Town, Zhongmu County, Henan Province. The test soil is tidal soil with light loam texture, medium to high fertility and uniform fertility. The nutrients in the tillage layer of the plot are: organic matter 16.2g / kg, total nitrogen 0.99g / kg, available phosphorus (P2O5) 11.3mg / kg, and available potassium (K2O) 89.6mg / kg. The previous crop was spinach with an output of 2100kg per mu. The test crop is rapeseed, and the variety is "Jinpin 535".

[0096] Experimental method: According to the actual planting situation of farmers, this experiment set up 6 treatments, arranged in random blocks, repeated three times, and the area of ​​the plot was set to 30m 2 .

[0097] Treatment 1: applying a water-soluble fertilizer containing 50% of the agricultural biostimulant containing fructooligosaccharides prepared in Example 2.

[0098] Treatment 2: applying a water-soluble fertilizer containing 50% of the fermentation product obtained in Comparative Example 1.

[0099] Treatment 3: applying a water-soluble fertilizer containing 50% of the fermentation product obtained in Comparative Example 2.

[0100] Treatment 4: applying a water-soluble fertilizer containing 50% of the fermentation product obtained in Comparative Example 3.

[0101] Treatment 5: applying a water-soluble fertilizer containing 50% of the fermentation product obtained in Comparative Example 4.

[0102] Treatment 6: applying a water-soluble fertilizer containing 50% of the fermentation product obtained in Comparative Example 5.

[0103] Each treatment used 100mL of fertilizer per mu and diluted it with 50kg of water. It was sprayed every 5 to 10 days during the rapid growth period of rapeseed, for a total of 2 times. The experiment was carried out on the basis of local conventional fertilization. Conventional fertilization is: 100kg of commercial organic fertilizer and 20kg of 51% triple compound fertilizer per mu, and 15kg of triple nitro compound fertilizer (28-5-5) was flushed during the rapid growth period. In addition to spraying fertilizer solution or clean water as required by the plan, other management measures were the same as those for general rapeseed field production. At the time of harvest, the actual yield and biological traits were recorded in different zones. The results are shown in Table 9:

[0104] Table 9 Comparison of field surveys of various treatments

[0105]

[0106]

[0107] (Note: The data in the table are the average of three treatments repeated three times)

[0108] The table shows that the use effect of the fermentation product is related to the fermentation raw material, sugarcane molasses Baume degree and fermentation temperature. Comparison between treatment 1 and treatments 2, 3, and 4 shows that when preparing the strain seed liquid, the sugarcane molasses Baume degree is controlled to 16-20°BX, the fermentation temperature is controlled to 25-35°C, and the fermentation temperature is controlled to 40-55°C during expansion fermentation. The fermentation product obtained has a better use effect. Comparison between treatment 1 and treatment 5 shows that the effect of using sugarcane molasses as a raw material is better than that of using white sugar as a raw material. Comparison between treatment 1 and treatment 6 shows that the fermentation effect of the strain of the present invention is better than that of conventional strains (Saccharomyces cerevisiae, Bacillus megaterium).

[0109] In summary, the present invention ferments sugarcane molasses by using Aspergillus oryzae and Aspergillus niger, and controls the fermentation conditions, including controlling the Baume degree, fermentation temperature and fermentation time of the sugarcane molasses, so that the obtained fermentation product has the effects of promoting the growth and development of crops and increasing the yield, and is suitable for promotion.

[0110] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A method for preparing agricultural biostimulants containing fructooligosaccharides using molasses, characterized in that: The agricultural biostimulant containing fructooligosaccharide is produced by fermenting sugarcane molasses with Aspergillus oryzae and / or Aspergillus niger.

2. The method for preparing agricultural biostimulants containing fructooligosaccharides using molasses according to claim 1, characterized in that: The fermentation comprises the following steps: (1) preparing a strain seed solution: using a sugarcane molasses dilution as a seed fermentation medium, inoculating the activated strain into the seed fermentation medium, and aerobically fermenting in a shaking incubator at 25-35° C. for 70-72 hours to obtain a strain seed solution; (2) Expansion fermentation: using sugarcane molasses diluted liquid as expansion fermentation medium, inoculating the strain seed liquid into the expansion fermentation medium, aerobically fermenting in a shaking incubator at 40-55° C. for 70-72 h, and sterilizing to obtain the product.

3. A method for preparing agricultural biostimulants containing fructooligosaccharides using molasses according to claim 2, characterized in that: In step (1), the Baume degree of the sugarcane molasses dilution is 10-16° BX.

4. The method for preparing agricultural biostimulants containing fructooligosaccharides using molasses according to claim 2, characterized in that: In step (1), the inoculation amount of the activated bacteria is 0.2-1% of the weight of the seed fermentation medium.

5. The method for preparing agricultural biostimulants containing fructooligosaccharides using molasses according to claim 2, characterized in that: In step (2), the Baume degree of the sugarcane molasses dilution is 20-80° BX.

6. The method for preparing agricultural biostimulants containing fructooligosaccharides using molasses according to claim 2, characterized in that: In step (2), the inoculation amount of the strain seed solution is 0.5-5% of the weight of the expansion fermentation medium.

7. Use of the agricultural biostimulant containing fructooligosaccharides according to any one of claims 1 to 6 in promoting the growth and development of crops and increasing their yield.

8. A fertilizer containing the agricultural biostimulant containing fructooligosaccharides according to any one of claims 1 to 6.

9. The fertilizer according to claim 8, characterized in that In the fertilizer, the content of the agricultural biostimulant containing fructooligosaccharide is 50-60%.

10. The fertilizer according to claim 8, characterized in that The fertilizer is applied by foliar spraying or root watering.

Images