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Extraction method of shark chondroitin sulfate

A technology of chondroitin sulfate and extraction method, which is applied in the direction of microorganism-based methods, food extraction, biochemical equipment and methods, etc., can solve the problems of unstable product quality, undiscovered shark chondroitin sulfate, time-consuming, etc., and achieve improved extraction Efficiency and product nutrition and health care efficacy, improving extraction rate and in vitro antioxidant capacity, improving extraction rate and the effect of nutrition and health care activity

Inactive Publication Date: 2020-07-31
马轩
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that shark cartilage is rich in chondroitin sulfate. Although some manufacturers have begun to produce shark chondroitin sulfate, there are still problems such as complex extraction process, time-consuming, high cost, unstable product quality, and low yield.
[0004] Using traditional dilute alkali, dilute alkali salt, dilute alkaline enzyme, etc. to extract chondroitin sulfate, the product yield is low, and the content of protein and nitrogen impurities is high
Patent technology 201811632893.6 discloses an extraction method of shark chondroitin sulfate, which uses a composite two-step enzymatic hydrolysis process to prepare shark chondroitin sulfate, which can replace the existing immobilized enzyme enzymatic hydrolysis process, and its product yield reaches 25-30%. The purity is up to 90-92%. However, dilute acid or dilute alkali is often used for pretreatment in the current extraction methods of shark chondroitin sulfate, which brings great environmental protection pressure to the extraction of shark chondroitin sulfate. At present, no direct use of enzymatic hydrolysis and A Report on the Extraction of Shark Chondroitin Sulfate by Microorganisms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for extracting shark chondroitin sulfate, specifically comprising the steps of:

[0034] (1) Pretreatment: crush the shark cartilage powder through a 100-mesh sieve, add pure water to dissolve; the amount of pure water added is 3 times the weight of the shark cartilage;

[0035] (2) Compound enzymolysis: add a compound enzyme preparation with a mass ratio of 2% shark cartilage powder to the filtrate in step (1), and perform enzymatic hydrolysis at 40-60°C for 4 hours; the compound enzyme preparation is papain, Mixture of trypsin and flavor protease, the mass ratio is 1:2:1;

[0036] (3) Fermentation broth preparation: add 2% glucose, 0.20% (NH 4 ) 2 SO 4 , 0.15%KH 2 PO 4 , 0.1%MgSO 4 , preparing a liquid fermentation medium;

[0037] (4) Sterilization: Sterilize at 121°C for 15 minutes;

[0038] (5) One-step aerobic fermentation: add Aspergillus oryzae with a mass ratio of 3% of the fermentation broth to the fermentation broth, and perform aerobic ferme...

Embodiment 2

[0044] A method for extracting shark chondroitin sulfate, specifically comprising the steps of:

[0045] (1) Pretreatment: crush the shark cartilage powder through a 100-mesh sieve, add pure water to dissolve; the amount of pure water added is 3 times the weight of the shark cartilage;

[0046] (2) Compound enzymolysis: add a compound enzyme preparation with a mass ratio of 2% shark cartilage powder to the filtrate in step (1), and perform enzymatic hydrolysis at 40-60°C for 4 hours; the compound enzyme preparation is papain, Mixture of trypsin and flavor protease, the mass ratio is 1:2:1;

[0047] (3) Fermentation broth preparation: add 2% glucose, 0.20% (NH 4 ) 2 SO 4 , 0.15%KH 2 PO 4 , 0.1%MgSO 4 , 0.5% astaxanthin to prepare a liquid fermentation medium;

[0048] (4) Sterilization: Sterilize at 121°C for 15 minutes;

[0049] (5) One-step aerobic fermentation: add Aspergillus oryzae with a mass ratio of 3% of the fermentation broth to the fermentation broth, and per...

Embodiment 3

[0055] A method for extracting shark chondroitin sulfate, specifically comprising the steps of:

[0056] (1) Pretreatment: crush the shark cartilage powder through a 100-mesh sieve, add pure water to dissolve; the amount of pure water added is 3 times the weight of the shark cartilage;

[0057] (2) Compound enzymolysis: add a compound enzyme preparation with a mass ratio of 2% shark cartilage powder to the filtrate in step (1), and perform enzymatic hydrolysis at 40-60°C for 4 hours; the compound enzyme preparation is papain, Mixture of trypsin and flavor protease, the mass ratio is 1:2:1;

[0058] (3) Fermentation broth preparation: add 2% glucose, 0.20% (NH 4 ) 2 SO 4 , 0.15%KH 2 PO 4 , 0.1%MgSO 4 , 1% astaxanthin to prepare a liquid fermentation medium;

[0059] (4) Sterilization: Sterilize at 121°C for 15 minutes;

[0060] (5) One-step aerobic fermentation: add Aspergillus oryzae with a mass ratio of 3% of the fermentation broth to the fermentation broth, and perfo...

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PUM

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Abstract

The invention discloses an extraction method of shark chondroitin sulfate, and belongs to the technical field of marine organisms. The shark chondroitin sulfate with the high oxidation resistance is researched and developed by taking shark cartilage powder as a raw material and integrating a biological enzymolysis technology, a mixed microbial fermentation technology and a modern biological separation technology, wherein a compound enzyme system adopted in the biological enzymolysis technology is one or a mixture of more of papain, trypsin, neutral protease, pepsin and flavourzyme, and fermentation strains for mixed microbial fermentation mainly include aspergillus oryzae, bacillus psychrosaccharolyticus and bacillus subtilis. The shark chondroitin sulfate prepared by the process is high in yield, has the relatively high oxidation resistance and the remarkable anti-tumor effect, and can be widely applied to medical health-care foods for preventing tumors and the like.

Description

technical field [0001] The invention relates to a method for extracting shark chondroitin sulfate, in particular to a new shark chondroitin sulfate product with high extraction rate and immune enhancement ability prepared by biological enzymolysis and microorganism synergy, belonging to the technical field of marine biology. Background technique [0002] Sharks are one of the oldest and longest-lived deep-sea marine creatures in the world. They have survived on the earth for 400 million years. They have a complete immune system and strong vitality. They are hardly infected by diseases. Can recover quickly, the chance of a shark getting cancer is about 1 in a million. The medicinal use of sharks in my country first appeared in the "Book of Materia Medica". Its cartilage is mainly composed of chondrocytes and collagen fibers, a large amount of proteoglycan and extracellular matrix. Headache, post-abdominal surgery pain, etc. In developed countries such as Europe, the United S...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P19/26C08B37/08A23L33/125C12R1/69C12R1/125C12R1/07
CPCA23L33/125A23V2002/00C08B37/0003C08B37/0069C12P19/26C12P39/00A23V2250/2042A23V2300/14
Inventor 马轩李鹏举王宁李静袁爱玲
Owner 马轩
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