Microbial agent for preventing and controlling lepidopteran pests and preparation method for microbial agent

A pest control and Lepidoptera technology, applied in the field of pesticides, can solve the problems of narrow insecticidal spectrum, strong antagonism between bacterial species, low control effect, etc., and achieve the effect of wide application prospect.

Inactive Publication Date: 2017-11-24
YANBIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a bacterial agent for the control of Lepidoptera pests, which solves the problems in the prior art that the application ran

Method used

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  • Microbial agent for preventing and controlling lepidopteran pests and preparation method for microbial agent

Examples

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Effect test

Embodiment 1

[0041] A bacterial agent for preventing and controlling Lepidoptera pests. The raw materials for its preparation are composed of the following components in parts by weight: 20 parts of Bacillus thuringiensis fermentation liquid, 15 parts of Brevibacillus spp. 10 parts of fermentation broth, 10 parts of Bacillus cereus fermentation broth, 5 parts of yeast fermentation broth;

[0042] Among them, the fermentation broth of Bacillus thuringiensis is obtained by culturing the pure strain of Bacillus thuringiensis to form spores and paraspore crystals, and produce crystal spores after isolation; It is obtained by culturing to the end of the logarithm; the fermentation broth of Bacillus coldolyticus is obtained by cultivating the pure strain of Bacillus coldolyticum to the end of the logarithm; the fermentation broth of Bacillus cereus is obtained by cultivating the pure strain of Bacillus cereus to Obtained at the end of the logarithm; Yeast fermentation liquid is obtained by cultu...

Embodiment 2

[0063] A bacterial agent for preventing and controlling Lepidoptera pests. The raw materials for its preparation are composed of the following components in parts by weight: 25 parts of Bacillus thuringiensis fermentation liquid, 15 parts of Brevibacillus spp. 10 parts of fermentation broth, 5 parts of Bacillus cereus fermentation broth, and 5 parts of yeast fermentation broth.

[0064] The preparation method is the same as in Example 1, except that the formula is changed into the antibacterial formula of Example 2 for the control of lepidopteran pests.

Embodiment 3

[0066] A bacterial agent for preventing and controlling Lepidoptera pests. The raw materials for its preparation are composed of the following components in parts by weight: 30 parts of Bacillus thuringiensis fermentation liquid, 10 parts of Brevibacillus spp. 5 parts of fermentation broth, 5 parts of Bacillus cereus fermentation broth, 10 parts of yeast fermentation broth.

[0067] The preparation method is the same as in Example 1, except that the formula is changed into the antibacterial formula of Example 3 for the control of lepidopteran pests.

[0068] The effect of the present invention will be further described below in conjunction with the application of the bacterial agent for the control of lepidopteran pests.

[0069] Since the bacterial agents prepared in Examples 1 to 3 have similar effects for controlling Lepidoptera pests, only the performance parameters measured after the preferred bacterial agents in Example 2 control Lepidoptera pests are used as illustratio...

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Abstract

The invention discloses a microbial agent for preventing and controlling lepidopteran pests. A raw material for preparing the microbial agent is prepared from the following components in parts by weight: 20 to 30 parts of fermented liquid of bacillus thuringlensis, 10 to 15 parts of fermented liquid of brevibacillus laterosporus, 5 to 10 parts of fermented liquid of bacillus psychrosaccharolyticus, 5 to 10 parts of fermented liquid of bacillus cereus and 5 to 10 parts of fermented liquid of yeasts. In addition, the invention further provides a preparation method for the microbial agent for preventing and controlling the lepidopteran pests. According to the microbial agent and the preparation method, five microbial agents are matched for use for the characteristics of the lepidopteran pests and each microbial agent, wherein the bacillus thuringlensis is a main insecticide, the brevibacillus laterosporus, the bacillus psychrosaccharolyticus and the bacillus cereus have synergistic effects on the bacillus thuringlensis, and the yeasts have protection and repair effects on leaves damaged by the lepidopteran pests. In addition, the microbial agent is free of residuals after use, free of drug resistance and environment-friendly.

Description

technical field [0001] The invention belongs to the technical field of insecticides, and in particular relates to a bacterial agent for controlling lepidopteran pests and a preparation method thereof. Background technique [0002] Insect damage is one of the important reasons for crop yield reduction, and reducing the damage of pests is an important way to increase the yield and quality of food and feed crops. According to statistics, the annual loss of global food and feed crops due to insect pests reaches 14%, and the economic losses directly caused by agricultural production are as high as hundreds of billions of dollars. Spraying chemical pesticides and biopesticides can certainly reduce the damage caused by pests to crops, but chemical pesticides are not safe for humans and animals and are likely to cause environmental pollution, while biopesticides are costly and relatively low in control efficiency. For a long time, large-scale spraying of chemical pesticides will no...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16A01N63/04A01N63/00A01P7/04C12R1/07C12R1/08C12R1/085
CPCA01N63/00A01N63/30C12N1/16C12N1/20A01N2300/00
Inventor 金大勇
Owner YANBIAN UNIV
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