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Production method of liquid water-soluble fertilizer containing saccharophilia bacillus agent and amino acid

A technology of Bacillus saccharophila and a production method, which is applied in the production field of liquid water-soluble fertilizer, can solve the problems of soil hardening and acidification, unable to form population effect, unable to exert the effect of microbial inoculum, etc., and achieves the promotion of root growth, remarkable product efficacy, Stable and controllable effect of fermentation process

Inactive Publication Date: 2016-12-14
WUZHOUFENG AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Due to the severe compaction and acidification of the soil and the erratic changes in the outside temperature, the microbial agents cultivated in the greenhouse are directly put into the soil, causing a large number of deaths because they cannot adapt to the harsh soil environment. The effect of the agent

Method used

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  • Production method of liquid water-soluble fertilizer containing saccharophilia bacillus agent and amino acid
  • Production method of liquid water-soluble fertilizer containing saccharophilia bacillus agent and amino acid
  • Production method of liquid water-soluble fertilizer containing saccharophilia bacillus agent and amino acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Grinding the washed and dried fresh sargassum into 50-60 mesh powder;

[0042]Put seaweed powder and water into a fermenter according to the mass ratio of 2:100, sterilize at 115°C for 35 minutes to obtain product A; and prepare seed liquid: inoculate Bacillus saccharophilus YIC~Alg3 into liquid medium and cultivate for 20 hours The preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No.12155; the liquid culture medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g A mixture of 20 g of agar and 1000 ml of distilled water, pH 7.2 to 7.4; the volume ratio of Bacillus saccharophilus YIC to Alg3 and the culture medium is 2:100. Add the seed solution to product A, wherein the mass ratio of the seed solution to product A is 1:100 to obtain product B; the product B is fermented at a temperature of 20°C and a pH of 5.5 to 6.0, and the fermentation time is After 7 days, product C was o...

Embodiment 2

[0045] The washed and dried fresh copper algae and fresh sargassum are compounded according to the mass ratio of 1:1, and ground into 50-60 mesh powder;

[0046] Seaweed powder and water are put into the fermenter according to the mass ratio of 4:100, and sterilized at 115°C for 25 minutes to obtain product A; and prepare seed liquid: inoculate Bacillus saccharophilus YIC~Alg3 into the liquid medium and cultivate for 22 hours The preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No.12155; the liquid culture medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g A mixture of 20 g of agar and 1000 ml of distilled water, pH 7.2 to 7.4; the volume ratio of Bacillus saccharophilus YIC to Alg3 and the culture medium is 5:100. Add the seed liquid to the product A, wherein the mass ratio of the seed liquid to the product A is 5:100 to obtain the product B; the product B is fermented at a tem...

Embodiment 3

[0048] Grinding the washed and dried fresh Sargassum into 50-60 mesh powder;

[0049] Put seaweed powder and water into a fermenter according to the mass ratio of 6:100, sterilize at 125°C for 35 minutes to obtain product A; and prepare seed liquid: inoculate Bacillus saccharophilus YIC~Alg3 into liquid medium and cultivate for 24 hours The preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No.12155; the liquid culture medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g A mixture of 20 g of agar and 1000 ml of distilled water, pH 7.2 to 7.4; the volume ratio of Bacillus saccharophilus YIC to Alg3 and the culture medium is 8:100. Add the seed solution to product A, wherein the mass ratio of the seed solution to product A is 4:100 to obtain product B; ferment product B at a temperature of 30°C and a pH of 7 to 7.5, and the fermentation time is 9 days , to obtain product C; adjust th...

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Abstract

The invention relates to a production method of a liquid water-soluble fertilizer containing a saccharophilia bacillus agent and amino acid. The method comprises: grinding dried seaweed into powder, adding the seaweed powder into water according to a certain ratio, putting the obtained product in a fermentation tank, and performing sterilization at a certain temperature for certain time; preparing a seed solution, which includes performing screening to obtain YIC-ALG3 saccharophilia bacillus as a fermentation strain for degrading alginic acid, inoculating a liquid medium with the YIC-ALG3 saccharophilia bacillus and performing cultivation for certain time; adding the seed solution into the fermentation tank according to a certain mass ratio; performing fermentation at a certain condition; adjusting the fermentation condition after the fermentation and performing preservation for certain time; adjusting the fermentation condition again and performing replenishment with the seed solution; performing filter pressing after the adjustment is carried out for three times to obtain a saccharophilia bacillus filtrate; and adding water, the saccharophilia bacillus filtrate and potassium glutamate in a stirring tank, and performing stirring till the mixture is completely dissolved to obtain the liquid water-soluble fertilizer containing a saccharophilia bacillus agent and amino acid. The method is stable and controllable in fermentation process, short in fermentation period, and obvious in product effect, and is suitable for large-scale production.

Description

technical field [0001] The invention relates to a production method of liquid water-soluble fertilizer. Background technique [0002] Seaweed fertilizer is a new type of organic fertilizer that uses natural seaweed as raw material, undergoes special biochemical processing, extracts and retains the natural active ingredients of seaweed, refines and concentrates. Seaweed fertilizer has the effect of improving crop yield and quality, resisting pests and diseases and adverse environment, and improving soil and water conservation. Its application in agricultural production has huge market demand and good development prospects. However, in order to ensure the quality of seaweed fertilizer, two key links of seaweed raw material and extraction process must be grasped. [0003] First of all, the quality of seaweed fertilizer depends on the raw materials used. At present, the raw materials used by seaweed fertilizer manufacturers are mainly brown algae, including Ascophyllum nodosum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/04C05F17/00C12N1/20C12R1/07C05G3/80
CPCC05F11/00C05F17/00C12N1/20C05G3/80C05F11/08Y02W30/40
Inventor 王学江曲胜杰于蒙爱李峰
Owner WUZHOUFENG AGRI SCI & TECH
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