9 results about "Potassium Glutamate" patented technology

The invention relates to the technical field of bioengineering, in particular to a preparation method and application of an antibacterial peptide, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID No: 1 and is named as L8. The preparation method of the antibacterial peptide L8 comprises the following steps: S1, obtaining an L8 target gene segment; connecting an L8 target gene segment with a pLC vector to obtain a connection product; transforming the connection product into an escherichia coli DH5-alpha competent cell, and screening positive clones to obtain a pLC-L8 recombinant plasmid; s2, taking the pLC-L8 recombinant plasmid as a substrate, and carrying out cell-free protein synthesis reaction to obtain the antibacterial peptide L8, the cell-free protein synthesis system comprises 175 mM of potassium glutamate, 10 mM of ammonium glutamate, 2.7 mM of potassium oxalate, 33 mM of PEP, 38 mM of compound amino acid, 10 mM of magnesium glutamate, 2% (w / v) PEG-8000, 5 nM of pLC-L8 recombinant plasmid, 11.25 mM of lactose, 33.33% (v / v) of crude extract, 100 mg / mL of escherichia coli tRNA and 2.5 nM of T7 RNA polymerase. The antibacterial peptide L8 is obtained by optimizing the antibacterial peptide LRGG, and compared with the LRGG, the antibacterial peptide L8 subjected to cell-free protein synthesis reaction expression has better antibacterial activity and biological safety.

The invention discloses a broad-spectrum plant-source disease-resistant excitant for rice blast and a preparation method thereof, and belongs to the technical field of rice blast prevention and control, in the broad-spectrum plant-source disease-resistant excitant, potassium glutamate serves as amino acid salt, not only provides a nitrogen source for plants, but also participates in the metabolic process in the plants and enhances the stress resistance of the plants. S-allyl-L-cysteine is a sulfur-containing amino acid derivative, has antioxidant and disease-resistant activity, and can stimulate a defense mechanism of plants and resist infection of magnaporthe oryzae. 2-piperidinecarboxylic acid has the effect of inhibiting growth of pathogenic bacteria and can directly interfere with cell wall synthesis of magnaporthe oryzae, so that diseases are relieved. Chitosan as a biological polysaccharide can induce plants to generate resistant substances, enhance the strength of plant cell walls, form a physical barrier and prevent invasion of pathogenic bacteria. Salicylic acid is an important signal molecule in a plant body and can activate systematic resistance of the plant and improve the overall disease resistance.

This invention provides a cultivation method to enhance the medicinal efficacy of Paris polyphylla, relating to the field of Paris polyphylla cultivation and management technology. The cultivation method mainly involves the combined application of a foliar fertilizer prepared from potassium dihydrogen phosphate, compound amino acids, potassium lauroyl glutamate, and pomegranate peel fermentation extract, and a plant compound fertilizer prepared from straw and manure compost, pomegranate peel fermentation material, and superphosphate. This invention overcomes the shortcomings of existing technologies, effectively ensuring the normal growth of Paris polyphylla while increasing the content of Paris saponins in its rhizomes, thus comprehensively improving the economic benefits of cultivation.

The invention discloses a moisturizing and repairing type hand sanitizer, and relates to the technical field of daily chemical products. The moisturizing and repairing type hand sanitizer is prepared from the following components in percentage by mass: 11 to 15 percent of a surfactant, 0.6 to 1.0 percent of an antibacterial composition, 4.1 to 5.7 percent of a moisturizing and skin moistening composition and 0.25 to 0.5 percent of an auxiliary agent, the surfactant is prepared from a plurality of materials selected from coco-glucoside, decyl glucoside and potassium cocoyl glutamate; the antibacterial composition is prepared from multiple of tea tree essential oil, polylysine hydrochloride and chitosan; the moisturizing and skin moistening composition is prepared from multiple of panthenol, shea butter, sodium hyaluronate and a honeysuckle flower extract; the auxiliary agent comprises xanthan gum and / or citric acid. The moisturizing and repairing type hand sanitizer provided by the invention realizes multiple advantages of naturalness, mildness, skin care, health and the like through a compound design of multiple systems of surface activity, antibiosis, moisturizing and skin moistening and the like.

The application discloses a moisturizing and repairing type hand sanitizer and relates to the technical field of daily chemical products. The moisturizing and repairing type hand sanitizer comprises the following components in percentage by mass: 11-15% of a surfactant, 0.6-1.0% of an antibacterial composition, 4.1-5.7% of a moisturizing and skin-moistening composition and 0.25-0.5% of an auxiliary agent. The surfactant comprises multiple kinds of cocoglucoside, decyl glucoside and potassium cocoyl glutamate. The antibacterial composition comprises multiple kinds of tea tree essential oil, polylysine hydrochloride and chitosan. The moisturizing and skin-moistening composition comprises multiple kinds of panthenol, shea butter, sodium hyaluronate and honeysuckle extract. The auxiliary agent comprises xanthan gum and / or citric acid. The moisturizing and repairing type hand sanitizer provided by the application realizes multiple advantages such as naturalness, mildness, skin care and health through the compounding design of multiple systems such as surfactants, antibacterial agents, moisturizing agents and skin-moistening agents.

This invention relates to the field of bioengineering technology, specifically to a method for preparing and applying an antimicrobial peptide. The amino acid sequence of the antimicrobial peptide is shown in SEQ ID No:1 and is named L8. A method for preparing antimicrobial peptide L8 includes S1. obtaining the L8 target gene fragment; ligating the L8 target gene fragment with a pLC vector to obtain a ligation product; transforming the ligation product into *E. coli* DH5α competent cells, screening for positive clones, and obtaining the pLC-L8 recombinant plasmid; S2. using the pLC-L8 recombinant plasmid as a substrate to perform a cell-free protein synthesis reaction to obtain antimicrobial peptide L8; the cell-free protein synthesis system includes 175 mM potassium glutamate, 10 mM ammonium glutamate, 2.7 mM potassium oxalate, 33 mM PEP, 38 mM complex amino acids, 10 mM magnesium glutamate, 2% (w / v) PEG-8000, 5 nM pLC-L8 recombinant plasmid, 11.25 mM lactose, and 33.33% (v / v) crude extract, 100 mg / mL *E. coli* tRNA, and 2.5 nM T7 RNA polymerase. This invention optimizes the antimicrobial peptide LR... GG Antimicrobial peptide L8 was obtained. The antimicrobial peptide L8 expressed without cell protein synthesis reaction was compared to LR. GG It has superior antibacterial activity and biosafety.

The invention belongs to the technical field of bioengineering, and particularly relates to a prokaryotic cell-free translation system buffer solution and application thereof. The prokaryotic cell-free translation system buffer solution comprises HEPES-KOH (pH 8.0), magnesium glutamate, ATP (adenosine triphosphate), GTP (glutathione triphosphate), potassium glutamate, creatine kinase and creatine phosphate. According to the present invention, the research accidentally discovers that the translation efficiency of the prokaryotic acellular translation system buffer solution constructed after the reduction of 28 unnecessary components is substantially the same as the translation efficiency of the original report, and the translation efficiency of the prokaryotic acellular translation system is significantly improved after the concentration is optimized, such that the good practical application value and the good prospect are provided.

The invention discloses a foliar fertilizer for inducing gray mold resistance of melon vegetables and a preparation method of the foliar fertilizer, and belongs to the technical field of foliar fertilizer.The foliar fertilizer comprises three main components, namely S-allyl-L-cysteine and 2-piperidinecarboxylic acid. As an amino acid potassium salt, potassium glutamate not only provides necessary potassium element for plants, but also enhances the growth vigor and disease resistance of the plants by promoting nitrogen metabolism in the plants. S-allyl-L-cysteine is an amino acid derivative containing a sulfur element and has the effect of inducing activity of resistance-related enzymes in plants, so that the resistance of the plants to gray mold is improved. 2-piperidinecarboxylic acid is a bioactive substance and can stimulate plants to generate disease-resistant related compounds, enhance the structural integrity of plant cells and resist invasion of pathogens. The potassium element provided by the potassium glutamate can enhance the thickness and strength of plant cell walls, and the combination of physical and chemical defense lines enables plants to more effectively resist the attack of gray mold.

The invention discloses an absorbent suitable for capturing CO2 by an electrochemical mediated amine regeneration method and application. The absorbent is prepared from organic amine, electrolyte and water, the organic amine comprises one or more of ethylenediamine, hydroxyethyl ethylenediamine, diethylenetriamine, triethylene tetramine, aminoethyl piperazine, benzylamine and N-methyldiethanolamine; the electrolyte is inorganic salt or organic salt; the inorganic salt electrolyte comprises one or more of sodium sulfate, potassium sulfate, sodium iodide, potassium iodide, sodium bromide, potassium bromide, magnesium sulfate, aluminum sulfate and zinc sulfate; and the organic salt electrolyte contains one or more of potassium glutamate, sodium glutamate, potassium glycinate, sodium glycinate, potassium alanine, sodium alanine, potassium sarcosine and sodium sarcosine. The concentrations of the organic amine and the electrolyte in the absorbent are 1-5 mo1 / kg and 0.2-1 mo1 / kg respectively. The absorbent has the characteristics of high stability, low corrosivity, large absorption capacity, stable absorption and desorption, good cycle performance and the like.