22 results about "Potassium Glutamate" patented technology

The invention discloses a disease-resistant water-soluble fertilizer and a preparation method thereof, belonging to the technical field of water-soluble fertilizers. The preparation method comprises: preparing first nanoparticles, preparing second nanoparticles, dissolving, suspending and mixing; the preparation of the first nanoparticles comprises the following steps: preparing a chitosan aqueous solution, acid treatment, adsorption, cross-linking, fixing zinc ions and chelation; the preparation of the second nanoparticles comprises the following steps: activation, grafting and coating; the suspension comprises mixing the first nanoparticles, the second nanoparticles and water, stirring at 60-65 DEG C, adding potassium humate, potassium L-glutamate and polyvinyl pyrrolidone, and continuing to stir; the disease-resistant water-soluble fertilizer prepared by the invention has good appearance, storage resistance and dilution effect, fast dilution speed, is not prone to flatulence when stored at high temperature, does not need frequent fertilization, and can improve the growth rate and yield of crops while improving the disease resistance of crops.

The invention relates to the technical field of bioengineering, in particular to a preparation method and application of an antibacterial peptide, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID No: 1 and is named as L8. The preparation method of the antibacterial peptide L8 comprises the following steps: S1, obtaining an L8 target gene segment; connecting an L8 target gene segment with a pLC vector to obtain a connection product; transforming the connection product into an escherichia coli DH5-alpha competent cell, and screening positive clones to obtain a pLC-L8 recombinant plasmid; s2, taking the pLC-L8 recombinant plasmid as a substrate, and carrying out cell-free protein synthesis reaction to obtain the antibacterial peptide L8, the cell-free protein synthesis system comprises 175 mM of potassium glutamate, 10 mM of ammonium glutamate, 2.7 mM of potassium oxalate, 33 mM of PEP, 38 mM of compound amino acid, 10 mM of magnesium glutamate, 2% (w / v) PEG-8000, 5 nM of pLC-L8 recombinant plasmid, 11.25 mM of lactose, 33.33% (v / v) of crude extract, 100 mg / mL of escherichia coli tRNA and 2.5 nM of T7 RNA polymerase. The antibacterial peptide L8 is obtained by optimizing the antibacterial peptide LRGG, and compared with the LRGG, the antibacterial peptide L8 subjected to cell-free protein synthesis reaction expression has better antibacterial activity and biological safety.

The invention discloses a broad-spectrum plant-source disease-resistant excitant for rice blast and a preparation method thereof, and belongs to the technical field of rice blast prevention and control, in the broad-spectrum plant-source disease-resistant excitant, potassium glutamate serves as amino acid salt, not only provides a nitrogen source for plants, but also participates in the metabolic process in the plants and enhances the stress resistance of the plants. S-allyl-L-cysteine is a sulfur-containing amino acid derivative, has antioxidant and disease-resistant activity, and can stimulate a defense mechanism of plants and resist infection of magnaporthe oryzae. 2-piperidinecarboxylic acid has the effect of inhibiting growth of pathogenic bacteria and can directly interfere with cell wall synthesis of magnaporthe oryzae, so that diseases are relieved. Chitosan as a biological polysaccharide can induce plants to generate resistant substances, enhance the strength of plant cell walls, form a physical barrier and prevent invasion of pathogenic bacteria. Salicylic acid is an important signal molecule in a plant body and can activate systematic resistance of the plant and improve the overall disease resistance.

This invention provides a cultivation method to enhance the medicinal efficacy of Paris polyphylla, relating to the field of Paris polyphylla cultivation and management technology. The cultivation method mainly involves the combined application of a foliar fertilizer prepared from potassium dihydrogen phosphate, compound amino acids, potassium lauroyl glutamate, and pomegranate peel fermentation extract, and a plant compound fertilizer prepared from straw and manure compost, pomegranate peel fermentation material, and superphosphate. This invention overcomes the shortcomings of existing technologies, effectively ensuring the normal growth of Paris polyphylla while increasing the content of Paris saponins in its rhizomes, thus comprehensively improving the economic benefits of cultivation.

The invention discloses a moisturizing and repairing type hand sanitizer, and relates to the technical field of daily chemical products. The moisturizing and repairing type hand sanitizer is prepared from the following components in percentage by mass: 11 to 15 percent of a surfactant, 0.6 to 1.0 percent of an antibacterial composition, 4.1 to 5.7 percent of a moisturizing and skin moistening composition and 0.25 to 0.5 percent of an auxiliary agent, the surfactant is prepared from a plurality of materials selected from coco-glucoside, decyl glucoside and potassium cocoyl glutamate; the antibacterial composition is prepared from multiple of tea tree essential oil, polylysine hydrochloride and chitosan; the moisturizing and skin moistening composition is prepared from multiple of panthenol, shea butter, sodium hyaluronate and a honeysuckle flower extract; the auxiliary agent comprises xanthan gum and / or citric acid. The moisturizing and repairing type hand sanitizer provided by the invention realizes multiple advantages of naturalness, mildness, skin care, health and the like through a compound design of multiple systems of surface activity, antibiosis, moisturizing and skin moistening and the like.

The invention relates to a method for efficiently and stably synthesizing mRNA in vitro, and belongs to the technical field of mRNA in-vitro synthesis. The invention creatively improves and optimizes the components of the buffer solution for capping reaction, and mixes polyglutamic acid, ethylenediaminetetraacetic acid and N-(1, 2-dicarboxyethyl)-D, L-asparaginic acid in a certain molecular weight range according to a certain weight ratio to obtain a compound stabilizer, so that the compound stabilizer can be used for preparing the buffer solution for capping reaction. According to the present invention, the buffer solution is prepared by mixing the buffer solution with potassium glutamate, magnesium glycinate and other components, can be directly compounded with a transcriptional mRNA mixed solution during actual use to catalyze the occurrence of the capping reaction, has high and stable capping rate, can omit the midway mRNA separation and purification operation compared to the prior art, and has the advantages of simple operation, easy operation, low cost and the like. The production efficiency is greatly improved, and efficient and stable mRNA in-vitro synthesis is realized.

The invention belongs to the technical field of food processing, and particularly relates to a method for preparing pickled beef through a potassium glutamate and trehalose synergistic salt and fat reduction technology and an obtained product. According to the composite salt system provided by the invention, under the synergistic effect of all the raw materials, not only can delicate flavor and salty taste be provided, but also sweet taste and slight bitter taste introduced by potassium glutamate can be balanced, and the defect of reduction of sodium chloride in flavor can be made up. Meanwhile, the texture of the pickled beef can be improved, the tenderness of the beef can be kept, and the beef is not easy to harden, so that the trehalose, the mushroom protein and the potassium glutamate are combined for use, the defects of salt-reduced pickled beef can be improved from the flavor and the texture, and the purpose of reducing sodium and not reducing saltiness of the pickled meat product is achieved.

The subject application pertains to compositions and methods for enhancing reverse transcriptase (RT) activity and / or reducing the inhibition of RT by inhibitors, such as formalin, tannic acid and / or heparin. In some embodiments, RT inhibition is reduced by the addition of potassium glutamate, histidine hydrochloride monohydrate, poloxamer 188, or any combination thereof to a reaction mixture comprising a polymerase. In other embodiments, RT is enhanced through the addition of a polyvinyl sulfonic acid sodium salt (PVSA) to a reaction mixture. The subject application also provides oligonucleotide primers for use in the reverse transcription of target sequences and its enhancement. These primers have high GC content or low GC content. Methods of using a RT inhibition reducer or a RT enhancer in a composition with an RNA template and RT improves RT yield, RT sensitivity, or RT tolerance to various chemicals are also provided.

The invention discloses an eco-friendly leaf surface blocking and controlling agent aiming at arsenic pollution of rice and a preparation method thereof. According to the invention, the potassium glutamate, the S-allyl-L-cysteine and the o-hydroxypiperidine acid are combined for use, so that the growth and the stress resistance of the rice are positively influenced. Potassium element in potassium glutamate participates in activation of various enzymes, so that photosynthesis and protein synthesis are promoted, and growth and development of rice are facilitated. The S-allyl-L-cysteine not only has the function of complexing arsenic ions, but also can be used as an antioxidant to scavenge free radicals, relieve oxidative stress reaction caused by arsenic and protect rice cells from being damaged. The o-hydroxypiperidine acid not only enhances the complexing effect, but also possibly improves the adaptability of the rice to arsenic pollution by influencing the ion balance in the rice body, optimizing the microenvironment in cells and improving the adaptability of the rice to arsenic pollution, so that the absorption and accumulation of the rice to arsenic are effectively reduced, and the overall health level of the rice is enhanced by improving the growth condition and physiological status of the rice.

The application discloses a moisturizing and repairing type hand sanitizer and relates to the technical field of daily chemical products. The moisturizing and repairing type hand sanitizer comprises the following components in percentage by mass: 11-15% of a surfactant, 0.6-1.0% of an antibacterial composition, 4.1-5.7% of a moisturizing and skin-moistening composition and 0.25-0.5% of an auxiliary agent. The surfactant comprises multiple kinds of cocoglucoside, decyl glucoside and potassium cocoyl glutamate. The antibacterial composition comprises multiple kinds of tea tree essential oil, polylysine hydrochloride and chitosan. The moisturizing and skin-moistening composition comprises multiple kinds of panthenol, shea butter, sodium hyaluronate and honeysuckle extract. The auxiliary agent comprises xanthan gum and / or citric acid. The moisturizing and repairing type hand sanitizer provided by the application realizes multiple advantages such as naturalness, mildness, skin care and health through the compounding design of multiple systems such as surfactants, antibacterial agents, moisturizing agents and skin-moistening agents.

A continuous or batch method of producing RNA using recycled components for in vitro transcription (IVT) comprises (a) producing RNA in a first composition comprising reagents or components for IVT; (b) separating the RNA from the first composition to leave the unconsumed components for IVT; (c) producing more RNA using the unconsumed components; concentration of electrolytes or “conducting agents” (e.g. NaCl, Urea, Guanidine HCl, Formamide, Arginine, Sodium Salicylate, DMSO, Thiourea, MgCl2, KCl, sodium acetate, potassium acetate, sodium nitrate, sodium bicarbonate, potassium glutamate, ammonium acetate and / or Tween-20) of at most 250mM. Preferably, the reagents are reused more than once. Preferably, the RNA transcription and the RNA separation are conducted at the same conducting agent concentration. Preferably, the method does not include buffer exchange or required the addition of extrinsic conducting agents. More preferably, the RNA is removed by an affinity (e.g. oligo-dT or oligo-dU) or phase (e.g. by temperature) separation method using beads, columns or membranes. The temperature phase separation may comprise cooling the first composition to form an upper phase comprising the unconsumed components for IVT and a lower phase comprising the RNA product. Cooling may comprise slow freezing (1 Kelvin per minute) and cold centrifugation steps.

This invention relates to the field of bioengineering technology, specifically to a method for preparing and applying an antimicrobial peptide. The amino acid sequence of the antimicrobial peptide is shown in SEQ ID No:1 and is named L8. A method for preparing antimicrobial peptide L8 includes S1. obtaining the L8 target gene fragment; ligating the L8 target gene fragment with a pLC vector to obtain a ligation product; transforming the ligation product into *E. coli* DH5α competent cells, screening for positive clones, and obtaining the pLC-L8 recombinant plasmid; S2. using the pLC-L8 recombinant plasmid as a substrate to perform a cell-free protein synthesis reaction to obtain antimicrobial peptide L8; the cell-free protein synthesis system includes 175 mM potassium glutamate, 10 mM ammonium glutamate, 2.7 mM potassium oxalate, 33 mM PEP, 38 mM complex amino acids, 10 mM magnesium glutamate, 2% (w / v) PEG-8000, 5 nM pLC-L8 recombinant plasmid, 11.25 mM lactose, and 33.33% (v / v) crude extract, 100 mg / mL *E. coli* tRNA, and 2.5 nM T7 RNA polymerase. This invention optimizes the antimicrobial peptide LR... GG Antimicrobial peptide L8 was obtained. The antimicrobial peptide L8 expressed without cell protein synthesis reaction was compared to LR. GG It has superior antibacterial activity and biosafety.

The present application relates to compositions and methods for enhancing reverse transcriptase (RT) activity and / or reducing inhibition of RT by inhibitors (e.g., formalin, tannic acid and / or heparin). In some embodiments, RT inhibition is reduced by adding potassium glutamate, histidine hydrochloride monohydrate, poloxamer 188, or any combination thereof to a reaction mixture comprising a polymerase. In other embodiments, RT is enhanced by adding sodium polyethylene sulfonate (PVSA) to the reaction mixture. The invention also provides oligonucleotide primers for reverse transcription of a target sequence and enhancement thereof. These primers have a high GC content or a low GC content. Also provided are methods of using an RT inhibition reducing agent or an RT enhancer in a composition having an RNA template and RT to improve RT yield, RT sensitivity, or RT tolerance to various chemicals.

To provide a down perm composition exhibiting excellent perm effect and fragrance, and allowing simple use.SOLUTION: The present invention relates to a composition for down perm and a method for down perm treatment using the same. The present invention provides a composition for down perm, comprising ethanolamine thioglycolate, cetearyl alcohol, ethanolamine, a surfactant, mineral oil, petrolatum, ammonium thioglycolate, propylene glycol, beeswax, steartrimonium chloride, paraffin, amodimethicone, guar hydroxypropyltrimonium chloride, hydrolyzed keratin, potassium cocoyl glutamate, hydrolyzed collagen, etidronic acid, sodium dilauramidoglutamide lysine, hexanediol, ethylhexanediol, fragrance, and water.SELECTED DRAWING: None

The invention belongs to the technical field of bioengineering, and particularly relates to a prokaryotic cell-free translation system buffer solution and application thereof. The prokaryotic cell-free translation system buffer solution comprises HEPES-KOH (pH 8.0), magnesium glutamate, ATP (adenosine triphosphate), GTP (glutathione triphosphate), potassium glutamate, creatine kinase and creatine phosphate. According to the present invention, the research accidentally discovers that the translation efficiency of the prokaryotic acellular translation system buffer solution constructed after the reduction of 28 unnecessary components is substantially the same as the translation efficiency of the original report, and the translation efficiency of the prokaryotic acellular translation system is significantly improved after the concentration is optimized, such that the good practical application value and the good prospect are provided.

The present invention provides a flavor enhancer or the like for food products, which contains one or more selections from the group consisting of potassium glutamate, potassium gluconate, glutamic acid, and gamma-glutamyl peptides.

The application relates to compositions and methods for enhancing reverse transcriptase (RT) activity and / or reducing RT inhibition by inhibitors (e.g., formalin, tannic acid and / or heparin). In some embodiments, potassium glutamate, histidine hydrochloride monohydrate, poloxamer 188 or any combination thereof are added to the reaction mixture comprising a polymerase to reduce RT inhibition. In other embodiments, RT is enhanced by adding polyvinyl sulfonate sodium salt (PVSA) to the reaction mixture. The application also provides reverse transcription for target sequences and oligonucleotide primers enhanced therefrom. These primers have high GC content or low GC content. Also provided is a method for using RT to suppress reducers or RT enhancers in compositions with RNA templates and RT to improve RT yield, RT sensitivity or RT tolerance to various chemicals.

The utility model relates to a double-decomposition electrodialysis device for preparing potassium glutamate, and belongs to the technical field of electrodialysis. A desalination liquid circulating pump I is connected with a membrane stack desalination liquid I water inlet, and a membrane stack desalination liquid I water outlet is connected with a desalination liquid circulating water tank I water outlet; a desalting liquid tank circulating pump II is connected with a membrane stack desalting liquid II water inlet; a membrane stack desalting liquid II water outlet is connected with a desalting liquid circulating water tank II water outlet; a concentrated solution circulating pump I is connected with a water inlet of a membrane stack concentrated solution I, and a water outlet of the membrane stack concentrated solution I is connected with a water outlet of a concentrated solution circulating water tank I; the concentrated solution circulating pump II is connected with a water inlet of a membrane stack concentrated solution II, and a water outlet of the membrane stack concentrated solution II is connected with a water outlet of the concentrated solution circulating water tank II. According to the utility model, the cost is reduced, the purity is improved, the membrane stack is protected, overhigh load is prevented, and the use requirements are met.

The invention discloses a foliar fertilizer for inducing gray mold resistance of melon vegetables and a preparation method of the foliar fertilizer, and belongs to the technical field of foliar fertilizer.The foliar fertilizer comprises three main components, namely S-allyl-L-cysteine and 2-piperidinecarboxylic acid. As an amino acid potassium salt, potassium glutamate not only provides necessary potassium element for plants, but also enhances the growth vigor and disease resistance of the plants by promoting nitrogen metabolism in the plants. S-allyl-L-cysteine is an amino acid derivative containing a sulfur element and has the effect of inducing activity of resistance-related enzymes in plants, so that the resistance of the plants to gray mold is improved. 2-piperidinecarboxylic acid is a bioactive substance and can stimulate plants to generate disease-resistant related compounds, enhance the structural integrity of plant cells and resist invasion of pathogens. The potassium element provided by the potassium glutamate can enhance the thickness and strength of plant cell walls, and the combination of physical and chemical defense lines enables plants to more effectively resist the attack of gray mold.

The invention discloses an absorbent suitable for capturing CO2 by an electrochemical mediated amine regeneration method and application. The absorbent is prepared from organic amine, electrolyte and water, the organic amine comprises one or more of ethylenediamine, hydroxyethyl ethylenediamine, diethylenetriamine, triethylene tetramine, aminoethyl piperazine, benzylamine and N-methyldiethanolamine; the electrolyte is inorganic salt or organic salt; the inorganic salt electrolyte comprises one or more of sodium sulfate, potassium sulfate, sodium iodide, potassium iodide, sodium bromide, potassium bromide, magnesium sulfate, aluminum sulfate and zinc sulfate; and the organic salt electrolyte contains one or more of potassium glutamate, sodium glutamate, potassium glycinate, sodium glycinate, potassium alanine, sodium alanine, potassium sarcosine and sodium sarcosine. The concentrations of the organic amine and the electrolyte in the absorbent are 1-5 mo1 / kg and 0.2-1 mo1 / kg respectively. The absorbent has the characteristics of high stability, low corrosivity, large absorption capacity, stable absorption and desorption, good cycle performance and the like.

The invention discloses a natural inducer for enhancing plant immunity to resist tomato early blight and a preparation process thereof. According to the invention, through scientific proportioning of the potassium glutamate, the S-allyl-L-cysteine, the 2-piperidinecarboxylic acid and other key components, the intrinsic immune mechanism of tomato plants is effectively stimulated. The potassium glutamate serving as amino acid salt not only provides nutrition required by plant growth, but also improves the resistance of plants to pathogenic bacteria by promoting cell division and enhancing cell wall strength. As a bioactive substance, S-allyl-L-cysteine can induce plants to generate disease-resistant related enzymes, such as chitinase and beta-1, 3-glucanase, and the enzymes can decompose cell walls of pathogenic bacteria so as to inhibit infection and diffusion of the pathogenic bacteria. 2-piperidinecarboxylic acid further strengthens the immune response of the plants by regulating the hormone balance in the plants, so that the tomato plants can quickly respond when facing early blight, and the occurrence and spreading of diseases are reduced.

The invention relates to the field of potassium polyglutamate preparation, and discloses a potassium polyglutamate preparation method and device, the device comprises a filter box, fixing feet, a fermentation box and a discharge pipe; the quantitative stirring and filtering unit is arranged in the fermentation box; the two fixing legs are symmetrically and fixedly arranged at the top of the filter box; the fermentation box is fixedly arranged on the fixing legs; the rack pipe is fixedly arranged at the bottom of the filter box, the switch assembly is driven by the driving assembly to be switched on and off, fermented materials directly fall into the filter box at the bottom, and the switch assembly is arranged to avoid the situation that the materials in the fermentation box directly fall into the filter box next to cause that the filter box cannot completely receive the materials. And through the arrangement, the situation that workers carry the materials back and forth to filter the materials is avoided.