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Freeze-dried vaccine of porcine transmissible gastroenteritis

A technology for transmissible gastroenteritis, which is applied in the field of porcine transmissible gastroenteritis freeze-dried vaccine, can solve the problems of immune failure, epidemic disease, potency discount, etc., and achieve the advantages of extended shelf life, simple operation and low raw material cost Effect

Inactive Publication Date: 2013-10-09
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The freeze-dried protective agent commonly used by domestic vaccine companies has a simple formula and poor protection performance. If stored at 2-8°C, the shelf life is only a short period of 3-6 months, and most of them need to be stored at -15°C. The long-term storage and long-distance transportation of vaccines are greatly restricted. In addition, the grassroots epidemic prevention departments lack the necessary freezing and cold storage facilities. It is easy to reduce the potency of vaccines due to improper storage temperature, which will eventually lead to immunization failure and epidemic epidemics.

Method used

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  • Freeze-dried vaccine of porcine transmissible gastroenteritis
  • Freeze-dried vaccine of porcine transmissible gastroenteritis
  • Freeze-dried vaccine of porcine transmissible gastroenteritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Preparation of lyoprotectant: divided into two parts, liquid A and liquid B.

[0025] 1) Preparation of liquid A: Weigh 48g of NZ-amine, 6g of monopotassium glutamic acid, 400g of sucrose, and 30g of hydrolyzed milk protein; dissolve the above ingredients in 800ml of water for injection in order, shake well after fully dissolving, and add water for injection After reaching 1000ml, shake well, filter and sterilize with a 0.22μm filter membrane, and store in a 37°C greenhouse for future use.

[0026] 2) Preparation of liquid B: Take 80g of gelatin, fully dissolve it with 800ml of water for injection at 70-80°C, add water for injection to 1000ml, stir well, autoclave at 121°C and 15 pounds for 30 minutes, and store at 37°C after sterilization.

[0027] 3) Proportion according to the volume ratio of liquid A: liquid B = 1:1, and mix well to get the heat-resistant freeze-drying protective agent.

[0028] 2. Preparation of porcine transmissible gastroenteritis freeze-drie...

Embodiment 2

[0035] 1. Preparation of lyoprotectant:

[0036] 1) Preparation of liquid A: Weigh 80g of NZ-amine, 10g of monopotassium glutamic acid, 500g of sucrose, and 50g of hydrolyzed milk protein; dissolve the above ingredients in 800ml of water for injection in order, shake well after fully dissolving, and add water for injection to 1000ml, sterilized by filtration with a 0.22μm filter membrane, and stored in a 37°C greenhouse for future use.

[0037] 2) Preparation of liquid B: Take 120g of gelatin, fully dissolve it with 800ml of water for injection at 70-80°C, add water for injection to 1000ml, autoclave at 121°C and 15 pounds for 30 minutes, and store at 37°C after sterilization.

[0038] 3) According to the ratio of liquid A: liquid B = 1:1, it can be used as a heat-resistant protective agent after mixing evenly.

[0039] 2. Vaccine preparation

[0040] 1. After porcine transmissible gastroenteritis virus is cultured, carry out sterility test and virus content determination, m...

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Abstract

The invention relates to a freeze-dried vaccine of porcine transmissible gastroenteritis. The vaccine comprises a porcine transmissible gastroenteritis virus serving as an antigen and a protective agent, wherein the protective agent comprises the following substances in percent concentration by mass: 2.4-4% of NZ-amine, 0.3-0.5% of monopotassium glutamate, 16-20% of sucrose, 6-20% of lactalbumin hydrolysate, 1-1.6% of gelatin and the balance of water. A freeze-drying protective agent used in the vaccine disclosed by the invention is low in raw material cost and simple to operate and can be produced on a large scale. The titer of the vaccine prepared by adopting the protective agent is greater than or equal to 105.7TCID50 (Tissue Culture Infectious Dose 50) / dose (national standard) after the vaccine is preserved for 24 months at 2-8 DEG C, so that the storage life of the vaccine is effectively prolonged.

Description

technical field [0001] The invention belongs to the technical field of veterinary vaccines, in particular to a freeze-dried vaccine for porcine transmissible gastroenteritis. Background technique [0002] Transmissible gastroenteritis of pigs (TGE) is a highly contagious viral infectious disease of pigs, which mainly causes vomiting, severe diarrhea and dehydration in piglets under 2 weeks of age. At present, in China, the incidence of the disease is increasing year by year, and some pig farms have a positive rate of more than 40%, which has become one of the main infectious diseases in intensive pig farms. The current pig industry has a high degree of intensification and high stocking density, and various stress factors can promote the occurrence of this disease. Among the many porcine infectious diseases at present, the occurrence of porcine transmissible gastroenteritis is relatively serious, which directly endangers the healthy development of pigs and brings huge econom...

Claims

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Application Information

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IPC IPC(8): A61K39/225A61K47/42A61K47/26A61K47/16A61K9/19A61P31/14A61P1/00A61K31/7036A61K31/43
Inventor 胡潇邹桂荣李芳王龙张维孙健
Owner YEBIO BIOENG OF QINGDAO
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