The invention relates to a non-toxic rodenticide Portland cement obstructing agent and a preparation method thereof. The non-toxic rodenticide Portland cement obstructing agent comprises paraffin, Portland cement, a cement accelerator, an adhesive, waxberry, Ilex latifolia, pomegranate, grain, an attractant and a preservative. The preparation method comprises the steps of heating the paraffin to prepare a wax liquid, drying and crushing waxberry, Ilex latifolia and pomegranate to prepare dry powder, and uniformly mixing and stirring the wax liquid, Portland cement, the waxberry dry powder, the Ilex latifolia dry powder, the pomegranate dry powder, the cement accelerator, the adhesive, the grain, the attractant and the preservative according to the formula proportion, filling the mixture into forming equipment, and pressing to obtain the non-toxic rodenticide Portland cement obstructing agent. The invention realizes no harm to the ecological environment, the components such as waxberry, Ilex latifolia and pomegranate are natural substances, the non-toxic rodenticide Portland cement obstructing agent is easy to mildew and deteriorate in the natural environment, is safe and harmless to human, poultry and livestock, has high deratization efficiency and obvious effect, and is applied to the technical field of rodent drug prevention and treatment.