Lyophilized vaccine for swine encephalitis B and preparation method thereof

A technology for porcine Japanese encephalitis and vaccines, which is applied in the direction of medical formulas, medical preparations with non-effective ingredients, and non-effective ingredients of high-molecular compounds, and can solve the problem of poor storage effect, low storage temperature, and Short shelf life and other issues, to achieve the effects of simple formula ingredients and preparation methods, good heat resistance, and high degree of aging resistance

Active Publication Date: 2013-09-18
YEBIO BIOENG OF QINGDAO
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  • Application Information

AI Technical Summary

Problems solved by technology

Thus solving the problems of conventional vaccines such as heat resistance, low storage temperature, short storage period and harsh freezing conditions during long-distance transportation, long-term storage and use, which cause a lot of energy waste.
However, the preservation effect of the currently used freeze-drying protective agent for porcine Japanese encephalitis vaccine is not good

Method used

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  • Lyophilized vaccine for swine encephalitis B and preparation method thereof
  • Lyophilized vaccine for swine encephalitis B and preparation method thereof
  • Lyophilized vaccine for swine encephalitis B and preparation method thereof

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Embodiment Construction

[0012] In the field of vaccine lyoprotectant preparation, the selection and ratio of the protective agent components have an important impact on the preservation effect of the vaccine. In order to enable the swine fever vaccine of the present invention to be preserved for a long time, the applicant has carried out long-term research on the components of the lyoprotectant of the present invention, and optimized the proportioning concentrations of each other, so that each component has the greatest complex matching effect.

[0013] For the used components of the lyoprotectant of the present invention, its properties are as follows:

[0014] 1. NZ-Amine AS, also known as the enzymatic hydrolyzate of casein;

[0015] 2. Glutamic acid monopotassium salt (L-glutamic acid potassium salt): It is a surfactant that can reduce the interfacial tension. Dehydrated and deformed, it can act as a wetting agent for active components during rehydration.

[0016] 3. Hydrolyzed milk protein, a...

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Abstract

The invention relates to a lyophilized vaccine for swine encephalitis B. The lyophilized vaccine consists of Japanese encephalitis virus serving as an antigen, and a protective agent, wherein the protective agent is prepared from the following substances in percentage by mass: 2.4-4% of NZ-amine, 0.3-0.5% of mono-potassium glutamate, 16-20% of sucrose, 6-20% of lactoalbumin hydrolysate, 1-1.6% of gelatin and the balance of water. According to the vaccine disclosed by the invention, the used lyophilized protective agent is made from raw materials which are low in cost, and the operation is simple, so that the vaccine can be produced in a large scale. After the vaccine prepared from the protective agent is stored for 24 months at the temperature of 2-8 DEG C, the potency of the vaccine is not smaller than 10<5.7>TCID50/head (Chinese Standard), so that the storage life of the vaccine is prolonged effectively.

Description

technical field [0001] The invention relates to a heat-resistant freeze-drying protective agent, in particular to a freeze-dried vaccine against porcine Japanese encephalitis and a preparation method thereof. Background technique [0002] Freeze-dried live vaccines are one of the main vaccines for preventing livestock and poultry infectious diseases. The freeze-dried protective agents commonly used by domestic vaccine companies are mainly sucrose, skimmed milk powder, sucrose, and gelatin in simple proportions. The formula is simple and the protection performance is poor. If stored at 2-8°C, the storage period is only 3-6 months, and most of them need to be stored at -15°C. This requires that the vaccine be stored, transported and used as low temperature as possible, otherwise the potency of the vaccine will be greatly reduced. In the process of vaccine production, transportation and use, if the cold chain system in any link is not properly controlled, the high temperature ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61K47/42A61K47/18A61K47/26A61P31/14
Inventor 邹桂荣管清江刘蕾申洪银陶晓珊张恒马爽郭莉莉
Owner YEBIO BIOENG OF QINGDAO
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