Lyophilized vaccine for swine encephalitis B and preparation method thereof

Abstract

The invention relates to a lyophilized vaccine for swine encephalitis B. The lyophilized vaccine consists of Japanese encephalitis virus serving as an antigen, and a protective agent, wherein the protective agent is prepared from the following substances in percentage by mass: 2.4-4% of NZ-amine, 0.3-0.5% of mono-potassium glutamate, 16-20% of sucrose, 6-20% of lactoalbumin hydrolysate, 1-1.6% of gelatin and the balance of water. According to the vaccine disclosed by the invention, the used lyophilized protective agent is made from raw materials which are low in cost, and the operation is simple, so that the vaccine can be produced in a large scale. After the vaccine prepared from the protective agent is stored for 24 months at the temperature of 2-8 DEG C, the potency of the vaccine is not smaller than 10<5.7>TCID50 / head (Chinese Standard), so that the storage life of the vaccine is prolonged effectively.

Description

technical field [0001] The invention relates to a heat-resistant freeze-drying protective agent, in particular to a freeze-dried vaccine against porcine Japanese encephalitis and a preparation method thereof. Background technique [0002] Freeze-dried live vaccines are one of the main vaccines for preventing livestock and poultry infectious diseases. The freeze-dried protective agents commonly used by domestic vaccine companies are mainly sucrose, skimmed milk powder, sucrose, and gelatin in simple proportions. The formula is simple and the protection performance is poor. If stored at 2-8°C, the storage period is only 3-6 months, and most of them need to be stored at -15°C. This requires that the vaccine be stored, transported and used as low temperature as possible, otherwise the potency of the vaccine will be greatly reduced. In the process of vaccine production, transportation and use, if the cold chain system in any link is not properly controlled, the high temperature ...

AI Technical Summary

Problems solved by technology

Thus solving the problems of conventional vaccines such as heat resistance, low storage temperature, short storage period and harsh freezing conditions during long-distance transportation, long-term storage and use, which cause a lot of energy waste.

However, the preservation effect of the currently used freeze-drying protective agent for porcine Japanese encephalitis vaccine is not good

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