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Extraction method of holothurin[v]

An extraction method and saponin technology, which are applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of restricting the large-scale utilization of sea cucumber saponins, low extraction rate of sea cucumber saponins, and high production cost, and improve the extraction rate. and in vitro antioxidant capacity, improving the extraction rate and antioxidant capacity, and enhancing the effect of in vitro antioxidant capacity

Inactive Publication Date: 2020-08-18
梁志勇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the existing technology, it is found that the extraction rate of sea cucumber saponins is low, the production cost is high, and it is difficult to realize industrialized production. Largest technical bottlenecks exploited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for extracting sea cucumber saponins, the specific process steps comprising:

[0039] (1) Cleaning: Clean the internal organs of the processed fresh sea cucumber with tap water, then wash with natural mineral water or purified water and drain for later use;

[0040] (2) Homogenization: put the sea cucumber viscera obtained in step (1) into a high-speed homogenizer, add natural mineral water or purified water, and perform high-speed homogenization until all can pass through a 2mm aperture circular sieve;

[0041] (3) Sterilization: Sterilize the homogenized sea cucumber viscera in a high-pressure steamer, cool and set aside;

[0042] (4) Compound enzymolysis: add a compound enzyme preparation with a mass ratio of 2% to the sterilized slurry, and stir the enzymolysis at 40-60°C for 30 minutes; the compound enzyme preparation is protease, non-starch polysaccharide enzyme and pomelo A glucosidase mixture, wherein the mass ratio of protease, non-starch polysaccharida...

Embodiment 2

[0051] A method for extracting sea cucumber saponins, the specific process steps comprising:

[0052] (1) Cleaning: Clean the internal organs of the processed fresh sea cucumber with tap water, then wash with natural mineral water or purified water and drain for later use;

[0053] (2) Homogenization: put the sea cucumber viscera obtained in step (1) into a high-speed homogenizer, add natural mineral water or purified water, and perform high-speed homogenization until all can pass through a 2mm aperture circular sieve;

[0054] (3) Sterilization: Sterilize the homogenized sea cucumber viscera in a high-pressure steamer, cool and set aside;

[0055] (4) Compound enzymolysis: add a compound enzyme preparation with a mass ratio of 2% to the sterilized slurry, and stir the enzymolysis at 40-60°C for 30 minutes; the compound enzyme preparation is protease, non-starch polysaccharide enzyme and pomelo A mixture of sidase, wherein the mass ratio of protease, non-starch polysaccharide...

Embodiment 3

[0064] A method for extracting sea cucumber saponins, the specific process steps comprising:

[0065] (1) Cleaning: Clean the internal organs of the processed fresh sea cucumber with tap water, then wash with natural mineral water or purified water and drain for later use;

[0066] (2) Homogenization: put the sea cucumber viscera obtained in step (1) into a high-speed homogenizer, add natural mineral water or purified water, and perform high-speed homogenization until all can pass through a 2mm aperture circular sieve;

[0067] (3) Sterilization: Sterilize the homogenized sea cucumber viscera in a high-pressure steamer, cool and set aside;

[0068] (4) Compound enzymolysis: add a compound enzyme preparation with a mass ratio of 2% to the sterilized slurry, and stir the enzymolysis at 40-60°C for 30 minutes; the compound enzyme preparation is a mixture of protease and non-starch polysaccharide enzyme , wherein the mass ratio of protease and non-starch polysaccharide enzyme is ...

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Abstract

The invention discloses an extraction method of holothurin, and belongs to the technical field of marine organisms. Sea cucumber deep processing waste sea cucumber viscera are used as raw materials; amodern biological separation technology, a biological enzymolysis technology, a mixed bacteria microbial fermentation technology and an ethanol precipitation and resin adsorption technology are integrated, a holothurian saponin biological product with high in-vitro oxidation resistance and an anti-cancer effect is developed, and mixed bacteria aerobic fermentation strains mainly comprise saccharomyces cerevisiae, bacillus subtilis and bacillus psychrosaccharolyticus. The obtained holothurian saponin has relatively strong antioxidant capacity and anti-cancer function, is strong in spectral property, and can be efficiently utilized in the fields of health care, foods, medicines and the like. The extraction method is high in holothurian saponin extraction rate, capable of achieving resourcereutilization of holothurian processing waste, convenient for industrial production and wide in market prospect.

Description

technical field [0001] The invention relates to a method for extracting sea cucumber saponins, in particular to a sea cucumber saponin biological product with high antioxidant capacity and nutritional and health care effects prepared by using sea cucumber internal organs as raw materials through collaborative fermentation of microorganisms, and belongs to the technical field of marine biology. Background technique [0002] Sea cucumber is an important marine invertebrate with tender meat and rich nutrition. It is a typical high-protein, low-fat food with high nutritional value and medicinal value. It is suitable for patients with hypertension, coronary heart disease, hepatitis, etc. For the elderly, it is a good food therapy product. It can not only enhance the body's immunity and hematopoietic function, but also inhibit the growth and metastasis of various molds and human cancer cells. As early as more than a thousand years ago, our country had the habit of eating sea cucum...

Claims

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Application Information

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IPC IPC(8): C12P33/20C12P39/00C12R1/865C12R1/125C12R1/07C12R1/225
CPCC12P33/20C12P39/00
Inventor 梁志勇王群马迪宋仁芳
Owner 梁志勇
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