Production method of water-soluble fertilizer containing bacillus saccharophilus and amino acid

A technology of Bacillus saccharophilus and production methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, organic fertilizers, etc., can solve the problems of inability to exert the effect of microbial agents, soil compaction and acidification, and inability to form population effects, etc. Achieving significant product efficacy, promoting root growth, and stable and controllable fermentation process

Active Publication Date: 2017-05-17
WUZHOUFENG AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Due to the severe compaction and acidification of the soil and the erratic changes in the outside temperature, the microbial agents cultivated in the greenhouse are directly put into the soil, causing a large number of deaths because they cannot adapt to the harsh soil environment. The effect of the agent

Method used

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  • Production method of water-soluble fertilizer containing bacillus saccharophilus and amino acid
  • Production method of water-soluble fertilizer containing bacillus saccharophilus and amino acid
  • Production method of water-soluble fertilizer containing bacillus saccharophilus and amino acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Grinding the washed and dried fresh sargassum into 50-60 mesh powder;

[0043] Put seaweed powder and water into a fermenter according to the mass ratio of 2:100, and sterilize at 115°C for 35 minutes to obtain product A; and prepare seed liquid: inoculate Bacillus saccharophilus YIC~Alg3 into liquid medium and cultivate for 20 hours The preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No12155; the liquid culture medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar The mixture of 20g and 1000ml of distilled water, pH7.2~7.4; the volume ratio of Bacillus saccharophilus YIC~Alg3 and culture medium is 2:100. Add the seed liquid to product A, wherein the mass ratio of seed liquid to product A is 1:100 to obtain product B; ferment product B at a temperature of 20°C and a pH of 5.5 to 6.0, and the fermentation time is After 7 days, product C was obtained; adjust the pH of ...

Embodiment 2

[0045] The washed and dried fresh copper algae and fresh sargassum are compounded according to the mass ratio of 1:1, and ground into 50-60 mesh powder;

[0046] Put seaweed powder and water into the fermenter according to the mass ratio of 4:100, sterilize at 115°C for 25 minutes to obtain product A; and prepare seed liquid: inoculate Bacillus saccharophilus YIC~Alg3 into the liquid medium and cultivate for 22 hours The preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No12155; the liquid culture medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar The mixture of 20g and 1000ml of distilled water, pH7.2~7.4; the volume ratio of Bacillus saccharophilus YIC~Alg3 and culture medium is 5:100. The seed liquid is added to product A, wherein the mass ratio of seed liquid to product A is 5:100, and product B is obtained; product B is fermented at a temperature of 25°C and a pH of 6....

Embodiment 3

[0049] Grinding the washed and dried fresh Sargassum into 50-60 mesh powder;

[0050] Put seaweed powder and water into a fermenter according to the mass ratio of 6:100, sterilize at 125°C for 35 minutes to obtain product A; and prepare seed liquid: inoculate Bacillus saccharophilus YIC~Alg3 into liquid medium and cultivate for 24 hours The preservation number of the Bacillus saccharophilus YIC~Alg3 is CGMCC No12155; the liquid culture medium is: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar The mixture of 20g and 1000ml of distilled water, pH7.2~7.4; the volume ratio of Bacillus saccharophilus YIC~Alg3 and culture medium is 8:100. Add the seed liquid to product A, wherein the mass ratio of seed liquid to product A is 4:100 to obtain product B; ferment product B at a temperature of 30°C and a pH of 7 to 7.5, and the fermentation time is 9 days , to obtain product C; adjust the pH of product C to 5...

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Abstract

The invention relates to a method for producing water-soluble fertilizer containing glycotropic bacillus and amino acid. Dried seaweed is ground into powder, the seaweed powder is added to water according to a certain proportion, and the mixture is placed in a fermentation tank and then sterilized at a certain temperature for a certain period of time; a seed solution is prepared; a fermentation strain YIC-ALG3 glycotropic bacillus capable of degrading alginic acid is obtained through screening, and a liquid culture medium is then inoculated with the fermentation strain for culture lasting a certain period of time; the seed solution is added into the fermentation tank according to a certain mass ratio to be fermented under a certain condition, the fermentation condition is adjusted after fermentation, the fermentation condition is adjusted again and the seed solution is supplemented after a certain period time of storage, glycotropic bacillus is obtained after three times of adjustment, and glycotropic bacillus and organic matter containing amino acid are evenly stirred to obtain the water-soluble fertilizer containing glycotropic bacillus and amino acid. The fermentation process is stable and controllable, fermentation period is short, product functions are remarkable, and the fertilizer is suitable for large-scale production.

Description

technical field [0001] The invention relates to a production method of water-soluble fertilizer containing bacillus saccharophilus and amino acid. Background technique [0002] Seaweed fertilizer is a new type of organic fertilizer that uses natural seaweed as raw material, undergoes special biochemical processing, extracts and retains the natural active ingredients of seaweed, refines and concentrates. Seaweed fertilizer has the effect of improving crop yield and quality, resisting pests and diseases and adverse environment, and improving soil and water conservation. Its application in agricultural production has huge market demand and good development prospects. However, in order to ensure the quality of seaweed fertilizer, two key links of seaweed raw material and extraction process must be grasped. [0003] First of all, the quality of seaweed fertilizer depends on the raw materials used. At present, the raw materials used by seaweed fertilizer manufacturers are mainly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/04C05F11/08C12N1/20C12R1/07C05G3/80
CPCC05C11/00C05F11/08C05G3/80C12N1/20C05F11/02
Inventor 王学江李峰马宁刘斌
Owner WUZHOUFENG AGRI SCI & TECH
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