Production process of bacillus psychrosaccharolyticus extracellular polysaccharide and application thereof

A technology of bacillus and exopolysaccharide, applied in the field of biology

Active Publication Date: 2012-04-04
青岛海大生物集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the current situation of global warming, shortage of water resources, increasingly serious soil desertification and heavy metal pollution, and the shortage of traditional chemical fertilizers and common microbial fertilizers, the present invention aims to prepare cell Exopolysaccharides, through screening and optimizing the medium, improving the production process, to obtain high-yield, high-purity exopolysaccharides; the exopolysaccharides are used in foliar fertilizers, drip irrigation fertilizers, and bacterial fertilizers in a certain proportion, which can not only retain water and improve the growth environment of crops, Moreover, it has strong adsorption capacity for heavy metals and strong anti-fungal disease, which can improve product quality and increase product output.

Method used

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  • Production process of bacillus psychrosaccharolyticus extracellular polysaccharide and application thereof
  • Production process of bacillus psychrosaccharolyticus extracellular polysaccharide and application thereof
  • Production process of bacillus psychrosaccharolyticus extracellular polysaccharide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Application example of HD-3 exopolysaccharide in foliar fertilizer

[0037] Take HD-3 exopolysaccharide 1.0‰, and other raw materials such as urea, dipotassium hydrogen phosphate, potassium sulfate, ferrous sulfate, boric acid, copper sulfate, zinc sulfate, ammonium molybdate, manganese sulfate, etc. to make nitrogen, phosphorus and potassium according to a certain ratio The content is 6%, 1.5%, 5%, respectively, and the total amount of trace elements is greater than 2%. The procedure is to dissolve 1.0g of exopolysaccharide in 1kg of tap water, turn on stirring and heat to 60°C to make it completely dissolved; then add other required raw materials, stir for 20 minutes until completely dissolved, pass through a 40-mesh sieve, and then sterilize and fill Put it in a 100mL plastic bottle and seal it, which is the finished product of polysaccharide foliar fertilizer.

Embodiment 2

[0038] Example 2: Application example of HD-3 exopolysaccharide in drip irrigation fertilizer

[0039] Take HD-3 exopolysaccharide 1.5‰, other raw materials such as urea, dipotassium hydrogen phosphate, potassium nitrate, humic acid, ferrous sulfate, boric acid, copper sulfate, zinc sulfate, ammonium molybdate, manganese sulfate, etc. Nitrogen, phosphorus and potassium content are 8%, 7%, 5%, respectively, the total amount of trace elements is more than 2%, and the drip irrigation fertilizer has an organic matter content of 30%. The procedure is to dissolve 1.5g of exopolysaccharide in 1kg of tap water, turn on and stir at 60°C to make it completely dissolved; then add biochemical fulvic acid, stir for 20 minutes until completely dissolved, add other required raw materials, and continue stirring until completely dissolved, After passing through a 40-mesh sieve, it is sterilized and filled in a 500mL plastic bottle, and sealed, which is the finished product of polysaccharide dr...

Embodiment 3

[0040] Example 3: Application example of HD-3 exopolysaccharide in bacterial fertilizer

[0041]1) Powdered bacterial fertilizer: Take HD-3 exopolysaccharide 1.2‰, other raw materials such as humic acid, attapulgite, plant ash, enteromorpha powder, diatomaceous earth, bentonite, bacterial liquid, etc., mix evenly according to a certain proportion, crush, Pass through a 100-mesh sieve, pack into bags and become a finished product of polysaccharide-containing powder bacterial fertilizer.

[0042] 2) Liquid bacterial preparation: take HD-3 exopolysaccharide 1.0‰, and other raw materials such as urea, potassium sulfate, dipotassium hydrogen phosphate, kelp puree, biochemical fulvic acid, emulsifier, bacterial liquid, etc., and mix evenly according to a certain ratio. Prepare a liquid bacterial preparation with an effective viable count greater than 1 billion / mL. The procedure is to dissolve 1.0‰ HD-3 exopolysaccharide in kelp puree, add biochemical fulvic acid, stir and keep warm...

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Abstract

The invention belongs to the technical field of biology and particularly relates to a production process of bacillus psychrosaccharolyticus extracellular polysaccharide and an application thereof to fertilizers. The bacillus psychrosaccharolyticus extracellular polysaccharide has good water-retaining property on leaves, stems and peripheral soil of plants, can adsorb heavy metal ions to reduce the environment pollution and can also inhibit various fungus diseases, the extracellular polysaccharide can be widely applied to the fertilizers of leaf surface fertilizers, drop irrigation fertilizers, bacterial fertilizers and the like through being used as a novel functional fertilizer, and the application prospects are wide. The production process of the bacillus psychrosaccharolyticus extracellular polysaccharide comprises the following steps that bacillus psychrosaccharolyticus HD-3 strains are obtained through separation, the seed culture liquid is prepared, and polysaccharide products are obtained through fermentation, extraction and drying. Because the culture medium recipe and the production process are optimized, the extracellular polysaccharide has high yield and low cost, and the finished products contain more than 90 percent of polysaccharide.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a production process and application of exopolysaccharide of Bacillus pyrolyticum. Background technique [0002] Microbial exopolysaccharides are water-soluble polysaccharides secreted by some special microorganisms outside the cell wall during growth and metabolism, easily separated from the bacteria, and secreted into the environment. They are secondary metabolites of microorganisms. Compared with ordinary polysaccharides, it has unique physical and chemical properties, and excellent specificity in structure and function. At present, the research on the biological activity of microbial exopolysaccharides is mainly concentrated in the fields of medicine, food, and chemical industry, such as antineoplastic drugs, functional foods that enhance immunity, flocculants and other chemical products; but for exopolysaccharides in agriculture, especially in There are still few re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C05G3/00C12R1/07
Inventor 乔来艳单俊伟宋海妹刁红霞
Owner 青岛海大生物集团股份有限公司
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