Method for inducing strains to participate in enzymolysis to produce alginate oligosaccharides and application of product

A technology of alginic acid oligosaccharides and alginic acid, which is applied in the application field of the alginic acid oligosaccharides, can solve the problems of few types of alginate lyases, instability and repeatability, and labor-consuming, so as to save manpower and energy costs , Facilitate industrial production and improve production efficiency

Active Publication Date: 2021-02-09
WUZHOUFENG AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although alginate lyase is an important seaweed tool enzyme with good application prospects, its related research is mostly limited to the laboratory level, and so far there is still no high-yield and low-cost high-efficiency enzyme preparation. Production applications are limited
For example, the alginate lyase mentioned in patents such as CN 107058423A and CN 108690857A has problems such as fewer types, low yield, poor stability of enzyme activity and high production cost, and it is difficult to realize the industrial production of alginate lyase
[0005] At present, seaweed fermentation mainly adopts the traditional composting fermentation process. This method has the disadvantages of cumbersome steps, long fermentation time, and labor-intensive. The bacteria in the fermentation process are uncontrollable, unstable, and poor in repeatability. The quality of the product is difficult to guarantee and it is easy to pollute the environment. , it is difficult to guarantee the requirements of high efficiency, simplicity, stability and uniformity, and environmental protection and cleanliness in industrialized production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] First, add sterilized water, pretreated brown algae, tryptone, yeast extract powder, sodium chloride, strain Q-4 for producing enzymes required for degrading alginic acid, and an inducer to the fermenter, adjust the pH of the system to 7.0, and control Ventilation, rotation speed and temperature were reacted for 22 hours, and then alginic acid oligosaccharides were collected;

[0028] Among them, the mass ratio between sterilized water, pretreated brown algae, tryptone, yeast extract powder, sodium chloride, the strain Q-4 used to produce the enzyme required for the degradation of alginic acid, and the inducer is: 65:8:0.65: 0.325:0.65:5:0.013.

[0029] The process control of the reaction process is divided into two stages. In the first stage, the ventilation rate is 0.3V / V min, the rotation speed is 300rpm, the temperature is 20°C, and the time is 7h; the second stage ventilation rate is 0.1V / V min, The rotational speed is 150 rpm, the temperature is 34°C and the time...

Embodiment 2

[0031] First, add sterilized water, pretreated brown algae, tryptone, yeast extract powder, sodium chloride, strain Q-4 for producing enzymes required for degrading alginic acid, and an inducer to the fermenter, adjust the pH of the system to 7.0, and control Ventilation, rotational speed and temperature were reacted for 23 hours, and then alginic acid oligosaccharides were collected;

[0032] Among them, the mass ratio between sterilized water, pretreated brown algae, tryptone, yeast extract powder, sodium chloride, the strain Q-4 used to produce the enzyme required for the degradation of alginic acid, and the inducer is: 65:8:0.65: 0.325:0.65:5:0.013.

[0033] The process control of the reaction process is divided into two stages. In the first stage, the ventilation rate is 0.4V / V min, the rotation speed is 300rpm, the temperature is 24°C, and the time is 8 h; the second stage ventilation rate is 0.1V / V min , the rotation speed is 150rpm, the temperature is 35°C and the tim...

Embodiment 3

[0035] First, add sterilized water, pretreated brown algae, tryptone, yeast extract powder, sodium chloride, strain Q-4 for producing enzymes required for degrading alginic acid, and inducer to the fermenter, adjust the pH of the system to 7.0, and control Ventilation, rotational speed and temperature were reacted for 24 hours, and then alginic acid oligosaccharides were collected;

[0036] Among them, the mass ratio between sterilized water, pretreated brown algae, tryptone, yeast extract powder, sodium chloride, the strain Q-4 used to produce the enzyme required for the degradation of alginic acid, and the inducer is: 70:8:0.7: 0.35:0.7:8:0.04;

[0037] The process control of the reaction process is divided into two stages. In the first stage, the ventilation rate is 0.4V / V min, the rotation speed is 300rpm, the temperature is 26°C, and the time is 8 h; the second stage ventilation rate is 0.2V / V min , the rotation speed is 150rpm, the temperature is 36°C and the time is 16...

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Abstract

The invention relates to a method for inducing strains to participate in enzymolysis to produce alginate oligosaccharides and application of a product. The method comprises the following steps: brownalgae are subjected to enzymolysis by using bacillus halosaccharovorans, and ventilatory capacity, rotating speed and temperature are controlled in different time periods to improve the process, so that the brown algae are degraded to produce the alginate oligosaccharides with the polymerization degree of 2-6. According to the process, the yield of the alginate oligosaccharides can be effectivelyincreased, the polymerization degree of the alginate oligosaccharides can be stably controlled, the cost is reduced, the process is low-carbon and environmentally friendly, the production efficiency is improved, and the industrial production of the alginate oligosaccharides is realized; and meanwhile, the alginate oligosaccharides produced with the method can be used as animal feed additives, theutilization efficiency of the alginate oligosaccharides is improved, the immunity of animals is enhanced, the growth and reproduction of the animals are promoted, and the quality of the animals is further improved.

Description

technical field [0001] The invention relates to a method for inducing bacteria to participate in enzymatic hydrolysis and production of alginic acid oligosaccharides. The present invention also relates to the application method of the alginic acid oligosaccharide. Background technique [0002] Alginic acid oligosaccharides with different monomer combinations and polymerization degrees exhibit different physiological activities and have different effects, such as anti-inflammatory and sterilization, lowering blood sugar and blood lipids, immune regulation, etc.; alginic acid oligosaccharides with a specific degree of polymerization also have certain degree of antitumor physiological activity. [0003] At present, the methods for extracting active substances from seaweed include enzymatic hydrolysis, physical methods and chemical methods. The chemical method mainly uses acid, alkali, etc. to destroy algal cells, so that the intracellular substances flow out, but it will dama...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/00C12P19/12C12P19/04C12N1/20A23K20/163A23K50/80C12R1/07
CPCC12P19/00C12P19/12C12P19/04C12N1/20A23K20/163A23K50/80Y02A40/818
Inventor 刘昌春李峰迟艳王修康孙泉磊林海南
Owner WUZHOUFENG AGRI SCI & TECH
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