Method for improving antioxidant ability of trepang saponin extracting solution

A technology of anti-oxidation ability and extraction liquid, applied in the field of marine biology, can solve the problems of low extraction rate of sea cucumber saponins, difficult to realize industrialized production, restricting the large-scale utilization of sea cucumber saponins, etc. The effect of antioxidant capacity

Inactive Publication Date: 2020-07-28
梁志勇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the existing technology, it is found that the extraction rate of sea cucumber saponins is low, the production cost is high, and it is difficult to realize industrialized production. Largest technical bottlenecks exploited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for improving the antioxidant capacity of a sea cucumber saponin extract, the specific process steps comprising:

[0040] (1) Cleaning: Clean the internal organs of the processed fresh sea cucumber with tap water, then wash with natural mineral water or purified water and drain for later use;

[0041](2) Homogenization: put the sea cucumber viscera obtained in step (1) into a high-speed homogenizer, add natural mineral water or purified water, and perform high-speed homogenization until all can pass through a 2mm aperture circular sieve;

[0042] (3) Sterilization: Sterilize the homogenized sea cucumber viscera in a high-pressure steamer, cool and set aside;

[0043] (4) Compound enzymolysis: add a compound enzyme preparation with a mass ratio of 2% to the sterilized slurry, and stir the enzymolysis at 40-60°C for 30 minutes; the compound enzyme preparation is protease, non-starch polysaccharide enzyme and fruit Glue enzyme mixture, wherein the mass ratio of p...

Embodiment 2

[0052] A method for improving the antioxidant capacity of a sea cucumber saponin extract, the specific process steps comprising:

[0053] (1) Cleaning: Clean the internal organs of the processed fresh sea cucumber with tap water, then wash with natural mineral water or purified water and drain for later use;

[0054] (2) Homogenization: put the sea cucumber viscera obtained in step (1) into a high-speed homogenizer, add natural mineral water or purified water, and perform high-speed homogenization until all can pass through a 2mm aperture circular sieve;

[0055] (3) Sterilization: Sterilize the homogenized sea cucumber viscera in a high-pressure steamer, cool and set aside;

[0056] (4) Compound enzymolysis: add 2% compound enzyme preparation and 3% mannan to the sterilized slurry, stir and enzymolyze at 40-60°C for 30 minutes; The compound enzyme preparation is a mixture of protease, non-starch polysaccharide enzyme and pectinase, wherein the mass ratio of protease, non-sta...

Embodiment 3

[0065] A method for improving the antioxidant capacity of a sea cucumber saponin extract, the specific process steps comprising:

[0066] (1) Cleaning: Clean the internal organs of the processed fresh sea cucumber with tap water, then wash with natural mineral water or purified water and drain for later use;

[0067] (2) Homogenization: put the sea cucumber viscera obtained in step (1) into a high-speed homogenizer, add natural mineral water or purified water, and perform high-speed homogenization until all can pass through a 2mm aperture circular sieve;

[0068] (3) Sterilization: Sterilize the homogenized sea cucumber viscera in a high-pressure steamer, cool and set aside;

[0069] (4) Compound enzymolysis: add 2% compound enzyme preparation and 4% mannan to the sterilized slurry, stir and enzymolyze at 40-60°C for 30 minutes; The compound enzyme preparation is a mixture of protease, non-starch polysaccharide enzyme and pectinase, wherein the mass ratio of protease, non-sta...

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PUM

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Abstract

The invention discloses a method for improving antioxidant ability of a trepang saponin extracting solution, and belongs to the technical field of marine organisms. Waste trepang internal organs obtained by deep processing of trepang are used as raw materials, and a modern bioseparation technique, a biological enzymolysis technique, a mixed bacterium microorganism fermentation technique and an ethanol precipitation and resin adsorption technique are integrated, so that the trepang saponin extracting solution high in in-vitro antioxidant ability and nutrient health-care efficacy is developed, wherein a compound enzyme preparation compound enzyme preparation is a mixture of one or more of proteases, non-starch polyase and pectinase, and mixed bacterium aerobic zymocyte mainly comprises candida utilis, bacillus licheniformis and bacillus psychrosaccharolyticus. The obtained trepang saponin has high antioxidant ability and high light spectrum properties, and can be efficiently utilized inthe fields of health care, foods, medicines and the like. The extraction method is high in extraction rate of the trepang saponin, can realize resource reusing of wastes obtained by processing of thetrepang, and is convenient for industrial production and broad in market prospects.

Description

technical field [0001] The invention relates to a method for improving the antioxidation ability of sea cucumber saponin extract, in particular to a sea cucumber saponin biological product with high antioxidation ability and nutrition and health care effect prepared by using sea cucumber viscera as a raw material through microbial cooperative fermentation, belonging to the technical field of marine biology. Background technique [0002] Sea cucumber is an important marine invertebrate with tender meat and rich nutrition. It is a typical high-protein, low-fat food with high nutritional value and medicinal value. It is suitable for patients with hypertension, coronary heart disease, hepatitis, etc. For the elderly, it is a good food therapy product. It can not only enhance the body's immunity and hematopoietic function, but also inhibit the growth and metastasis of various molds and human cancer cells. As early as more than a thousand years ago, our country had the habit of ea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/10A61K35/616A61P39/06C12P33/20C12N1/16C12N1/20C12R1/72C12R1/10C12R1/07
CPCA23L33/10A23V2002/00A61K35/616A61P39/06C12N1/16C12N1/20C12P33/20A23V2200/30A23V2250/2042
Inventor 梁志勇王群马迪宋仁芳
Owner 梁志勇
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