Fermentation culture medium and method for preparing mupirocin by virtue of fermentation culture medium

Abstract

The invention discloses a fermentation culture medium and a method for preparing mupirocin by virtue of the fermentation culture medium. The method comprises the following steps: breeding pseudomonasfluorescence with good growth state, vigorous vitality and strong metabolic capability, blending a culture medium, carrying out strain activation on pseudomonas fluorescence, and selecting optimal strains for later use; and inoculating the strains to a special seed culture medium so as to increase the quantity of excellent pseudomonas fluorescence, carrying out culturing to a logarithmic phase soas to obtain a seed bacteria solution, and finally, inoculating the seed bacteria solution to the special fermentation culture medium. According to the fermentation culture medium, sufficient nutrients can be provided for pseudomonas fluorescence, the growth and metabolism speeds of pseudomonas fluorescence can be increased, a fermentation liquid containing mupirocin is obtained, and the content of mupirocin in the fermentation liquid is over 6000ug/mL.

Description

Technical field [0001] The present invention relates to the field of medicine, in particular to a fermentation medium and a method for preparing mupirocin from the fermentation medium. Background technique [0002] Mupirocin, also known as Pseudomonas acid A, is a kind of gram-positive bacteria [such as Staphylococcus aureus (Staphylococcus aureus), Streptococcus pyogenes), Streptococcus pneumoniae (Streptococcus) pneumoniae), Klebsiella pneumoniae (Klebsiella pneumoniae)) and some Gram-negative bacteria (such as Haemophilus influenzae (Haemophilus influenzae), Neisseria gonorrhoeae (Neisseria gonorrhoeae)] antibiotics with growth inhibitory effect [A.Ward, DM Campoli-Richards, Drugs 32, 425-444 (1986)]. By inhibiting isoleucine-tRNA synthase, it affects the peptide synthesis of pathogenic bacteria. An advantageous feature of this antibiotic is that it has very low toxicity to humans and animals and it was negative in the Ames test. At present, mupirocin is mostly used in the t...

AI Technical Summary

Problems solved by technology

[0003] It is known that Pseudomonas fluorescens can produce mupirocin, but the fermentation level of the current preparation method is low, and the content of mupirocin in the obtained fermentation broth is generally only 3000-4000ug / ml

Images