Culture medium for increasing speed of nitrite oxidation by nitrobacteria

A technology for oxidizing nitrite and culture medium, applied in the field of culture medium to effectively increase the rate of nitrifying bacteria oxidizing nitrite, can solve problems such as economic loss, excessive accumulation of nitrite poisoning, lack of nitrifying bacteria products, etc., and achieve the degradation rate The effect of improving and speeding up the growth metabolism

Inactive Publication Date: 2010-05-19
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the severe damage to the micro-ecological environment of the water body in recent years, the excessive accumulation of nitrite poisoning incidents in large areas of the water area frequently broke out, directly or indirectly causing huge economic losses in our country.
However, the nitrifying bacteria products on the market in my country are extremely scarce, and most of them are substitutes for other types of low-efficiency mixed microorganisms, such as photosynthetic bacteria, bacillus, etc.
Especially the lack of research on nitrite degradation in eutrophic water

Method used

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  • Culture medium for increasing speed of nitrite oxidation by nitrobacteria

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Dissolve 2.1 g of glycerin, 1.3 g of yeast extract, 1.0 g of sodium bicarbonate, 1.9 g of sodium nitrite, 0.1 g of sodium carbonate, 0.1 g of sodium chloride, 0.1 g of potassium dihydrogen phosphate, 0.1 g of magnesium sulfate, and 0.005 g of ferrous sulfate was used as a culture medium.

[0022] Nitrobacter strains (Nitrobacter hamburgensis, Nitrobacter winogradskyi, etc.) were activated and scaled up to 1L step by step, the temperature was 30°C, the dissolved oxygen was controlled to be greater than 5.0mgO2 / L, and an inoculum of 10% by volume was inserted into the above medium. The degradation rate of the cultivated bacterial agent was 926.4mgNO2-N / gMLSS·d. In the same batch, conventional medium was used (every 1000 mL of water contained 2.0 g / L of sodium bicarbonate, 0.5 g / L of sodium nitrite, 0.2 g / L of potassium dihydrogen phosphate, 0.05 g / L of magnesium sulfate, and 0.1 g / L of iron sulfate. ) to obtain a degradation rate of 580.0mgNO 2 -N / gMLSS·d. The results ...

Embodiment 2

[0024] Dissolve glycerin 3.1, yeast extract 1.9g, sodium bicarbonate 2.0g, sodium nitrite 3.2g, sodium carbonate 0.8g, sodium chloride 0.5g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.5g and Ferrous sulfate 0.05g was made into culture medium. Nitrobacter strains (Nitrobacter hamburgensis, Nitrobacter winogradskyi, etc.) were activated and scaled up to 1L step by step, the temperature was 28°C, the dissolved oxygen was controlled to be greater than 5.0mgO2 / L, and the inoculum of 5% by volume was inserted into the above medium. The degradation rate of the cultivated bacterial agent was 947.8mgNO2-N / gMLSS·d. In the same batch, conventional medium was used (every 1000 mL of water contained 2.0 g / L of sodium bicarbonate, 0.5 g / L of sodium nitrite, 0.2 g / L of potassium dihydrogen phosphate, 0.05 g / L of magnesium sulfate, and 0.1 g / L of iron sulfate. ) to obtain a degradation rate of 580.0mgNO 2 -N / gMLSS·d. The result shows that the degradation rate of the nitrifying...

Embodiment 3

[0026] Dissolve 2.6g of glycerin, 1.6g of yeast extract, 1.7g of sodium bicarbonate, 2.7g of sodium nitrite, 0.5g of sodium carbonate, 0.4g of sodium chloride, 0.4g of potassium dihydrogen phosphate, and 0.2g of magnesium sulfate in 1000mL of water. and ferrous sulfate 0.01g to make culture medium. Activate the nitrifying bacteria (Nitrobacter hamburgensis, Nitrobacter winogradskyi, etc.) and scale up to 1L step by step. The temperature is 32°C, the dissolved oxygen is controlled to be greater than 5.0mg / L, and the inoculum of 8% by volume is inserted into the above medium. The degradation rate of the cultivated bacterial agent was 1194.1mgNO2-N / gMLSS·d. In the same batch, conventional medium was used (every 1000 mL of water contained 2.0 g / L of sodium bicarbonate, 0.5 g / L of sodium nitrite, 0.2 g / L of potassium dihydrogen phosphate, 0.05 g / L of magnesium sulfate, and 0.1 g / L of iron sulfate. ) to obtain a degradation rate of 580.0mgNO 2 -N / gMLSS·d. The results show that th...

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Abstract

The invention discloses a culture medium for improving the speed of nitrifying bacteria oxidizing nitrites. The composition of the culture medium is that every 1000 milliliters of water contain 2.1 to3.1 grams of glycerin, 1.3 to 1.9 grams of yeast extract, 1.0 to 2.0 grams of baking soda,1.9 to 3.2 grams of sodium nitrite, 0.1 to 0.8 grams of sodium carbonate, 0.1 to 0.5 grams of sodium chloride, 0.1 to 0.5 grams of potassium dihydrogen phosphate, 0.1 to 0.5 grams of magnesium sulfate and 0.005 to 0.05 grams of ferrous sulfate, wherein the glycerin and the yeast extract are better to be supplemented at the late stage of fermentation. The culture medium ensures obvious promotion of the speed of growth metabolism of the nitrifying bacteria and significant increasing of the degradation rate of the nitrites, thereby the average degradation rate of the invention is increased 40% to 127% in comparison with a conventional culture medium 900-1320mgNO2-N / gMLSS.d. Tests of small water body and real water body prove that bacteria cultured by the invention can continuously and high effectively degrade nitrites in polluted water body.

Description

technical field [0001] The invention relates to a nitrifying bacteria fermentation medium, in particular to a medium for effectively improving the rate of nitrifying bacteria oxidizing nitrite. Background technique [0002] Nitrifying bacteria (Nitrite Oxidizing Bacteria) are an important class of microorganisms that degrade nitrite into nitrate in the natural nitrogen cycle. Due to the severe damage to the micro-ecological environment of water bodies in recent years, the excessive accumulation of nitrite in large areas and poisoning incidents broke out frequently, which directly or indirectly caused huge economic losses in our country. However, the nitrifying bacteria products on the market in my country are extremely scarce, and most of them are substitutes for other types of low-efficiency mixed microorganisms, such as photosynthetic bacteria and bacillus. Especially the lack of research on nitrite degradation in eutrophic water. Therefore, the research and development ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 林炜铁任杰
Owner SOUTH CHINA UNIV OF TECH
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