A kind of fermentation medium and method for preparing mupirocin from fermentation medium

Abstract

The invention discloses a fermentation medium and a method for preparing mupirocin from the fermentation medium. Firstly, Pseudomonas fluorescens is bred in a good growth state, vigorous in vitality and strong in metabolism. The bacteria are activated to prepare for the selection of the best strains; then inoculate them on a special seed medium to expand the number of excellent Pseudomonas fluorescens bacteria, and cultivate them to the logarithmic phase. The seed bacterium liquid is obtained, and finally the seed bacterium liquid is inoculated on a special fermentation medium. The culture medium provided by the present invention can not only provide sufficient nutrition for Pseudomonas fluorescens, but also accelerate the growth and metabolism of Pseudomonas fluorescens, so as to obtain a fermented liquid containing mupirocin, and mupirocin in the fermented liquid The content is above 6000ug / mL.

Description

technical field [0001] The invention relates to the field of medicines, in particular to a fermentation medium and a method for preparing mupirocin from the fermentation medium. Background technique [0002] Mupirocin, also known as pseudomonic acid A, is a gram-positive bacteria (such as Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae) pneumoniae), Klebsiella pneumoniae] and some Gram-negative bacteria [such as Haemophilus influenzae, Neisseria gonorrhoeae] have growth-inhibitory antibiotics [A.Ward, D.M. Campoli-Richards, Drugs 32, 425-444 (1986)]. By inhibiting isoleucine-tRNA synthase, it affects the peptide synthesis of pathogenic bacteria. An advantageous feature of this antibiotic is that it has very low toxicity to humans and animals and it is negative in the Ames (Ames) test. At present, mupirocin is mostly used in human therapy to treat skin infections (such as pustules) pyoderma), nasal and outer ear infections, acne, burns, eczema, psor...

AI Technical Summary

Problems solved by technology

[0003] It is known that Pseudomonas fluorescens can produce mupirocin, but the fermentation level of the current preparation method is low, and the content of mupirocin in the obtained fermentation broth is generally only 3000-4000ug / ml

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