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CZ-81 strain of 'Baibachi' bacterium and artificial culture method

A technology of artificial cultivation of white rake tooth fungus, applied in the direction of fungi, etc., can solve the problems of no cultivation method, poor stability, weak metabolic function, etc., and achieve the effect of scientific control of the production process, strong stability, and increased content

Inactive Publication Date: 2005-06-15
TONGHUA JITONG PHARMA
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  • Abstract
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Problems solved by technology

[0003] The fermented extracts isolated and purified from the strains of white rake tooth bacteria have good clinical effects on the treatment of chronic glomerulonephritis. The existing strains have poor stability and weak metabolic function. How to artificially cultivate high-quality white rake Dental bacteria strains, has not been a good culture method

Method used

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  • CZ-81 strain of 'Baibachi' bacterium and artificial culture method
  • CZ-81 strain of 'Baibachi' bacterium and artificial culture method
  • CZ-81 strain of 'Baibachi' bacterium and artificial culture method

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Embodiment Construction

[0073] A strain with stable performance obtained through isolation, purification, screening, and systematic selection and evaluation from the dead standing wood of broad-leaved trees or dead wood in the mixed coniferous and broad-leaved forest belt of Changbai Mountains, China. The method is:

[0074] (1) Level 1 slant strains:

[0075] Medium: Comprehensive PDA Medium: Glucose 2%, Potato 20%, Agar 2%, KH 2 PO 4 0.3%, MgSO 4 0.2%, VB1 0.05%, PH6.5 (before elimination).

[0076] Set up special personnel, special boxes (refrigerators), and special record management. Check and record the strain performance and aging condition at any time. Do a good job of marking and recording the subculture of the transfer tube, and the generation (number of passages) of the strain used in production must be clear. Abnormal and aging strains should be eliminated in time and should not be used in production.

[0077] The culture temperature of the slant strain is 26°C, and the mycelia co...

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Abstract

A Baipichi strain CZ-81 (CGMCC No.1016) is disclosed, which features high stability, metablitic power and safety. Its artificial culture method includes slant culture, shake culture, seeding-tank culture, fermenting and extracting. Its advantages are short time dicreased by 20%, and high content of active components.

Description

Technical field: [0001] The invention relates to a method for cultivating high-performance strains cultured by submerged fermentation of fungal bioengineering, that is, the bacterial strain "CZ-81" of the fungi. Background technique: [0002] Irpex lacteus Fr. is a kind of fungus belonging to the family Irpex lacteus, the genus Irpex lacteus, and the species Irpex lacteus, also known as white sac hole. Synonyms: Hirschioporus lacteus (Fr.) Teng; Deng Shuqun, Fungi in China, 484.1963. Its fruiting bodies are born on dead standing or fallen wood of broad-leaved trees. Morphological characteristics: Basidiocarp (fruiting body) flattened, expanded, white, soft leathery; or flattened and rolled up, the rolled part is 8-15×10-20mm. Sometimes anatropous, stacked in the shape of compound tiles, fan-shaped, with a narrow base, 2-4cm in transverse diameter, and 2-3mm in original; cover surface is milky white, with short hairs, silky luster, ring pattern, thin edge, Recurved, wavy t...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 宋启印
Owner TONGHUA JITONG PHARMA
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