Culture method for promoting autotrophy microalgae neutral lipid accumulation

A culture method and neutral lipid technology, applied in the field of oil-rich microalgae cultivation, to achieve the effects of simplifying the process, promoting and accumulating efficiency and accumulation, and improving accumulation efficiency and accumulation

Active Publication Date: 2013-10-16
NINGBO UNIV
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  • Application Information

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Problems solved by technology

But so far, there is no method to promote the accumulation of n...

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  • Culture method for promoting autotrophy microalgae neutral lipid accumulation
  • Culture method for promoting autotrophy microalgae neutral lipid accumulation

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Embodiment 1

[0018] Example 1: Neutral lipid accumulation culture of Thalassiosira pseudonana oil-rich strain NMBguh005

[0019] 1. Cell proliferation culture:

[0020] Take natural seawater for filtration and disinfection, add KNO separately 3 100 mg / L, K 2 HPO 4 10 mg / L, MnSO 4 0.25 mg / L, FeC 6 h 5 o 7 2.5 mg / L, Na 2 EDTA 20 mg / L, V B12 0.5 mg / L, V B1 5 mg / L of each substance formed microalgae culture fluid. Thalassiosira pseudonana was inoculated into the above culture medium for cell proliferation culture. The salinity of the culture solution is 23‰, the culture temperature is 25°C, and the light is 4000 lx (D:L=12:12). The culture environment is obtained by a light incubator with a fluorescent light source. The volume of the culture water body is 500mL, and 6 parallel. Three replicates of 2 mL were sampled daily for microscopic counting to follow the growth cycle. The process of cell proliferation is shown in figure 1 (0-12d) shown. On the 12th day of culture, 6 p...

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Abstract

The present invention provides a culture method for promoting autotrophy microalgae neutral lipid accumulation, wherein microalgae having neutral lipid accumulation metabolism capability at a proliferation later stage is subjected to neutral lipid accumulation culture under a 400-550 nm light wave condition. According to the method, under the premise of proliferation culture of the obtained first step cells to achieve the maximum biomass, microalgae neutral lipid metabolism rapidly responses in a short time through temperature reduction on the microalgae liquid and a synergism effect of the illumination condition so as to achieve a purpose of promotion and increase of the neutral lipid accumulation efficiency and the neutral lipid accumulation amount. The blue filter membrane is a plastic film, and can be subjected to large-scale application and modification. The nitrogen limitation stress condition can be achieved through N/P ratio inversion regulation without a microalgae and media separation process. In addition, the process in the large-scale production can be simplified.

Description

technical field [0001] The invention belongs to the technical field of oil-rich microalgae cultivation, and in particular relates to a cultivation method for promoting the accumulation of neutral lipids in autotrophic microalgae. Background technique [0002] Under the impact of the global energy crisis, human beings have been extensively conducting research on finding environmentally friendly new renewable energy sources. Autotrophic single-cell microalgae have become important materials for new energy due to their fast growth, high biomass, and excellent synergistic ecological effects. Among them, microalgae whose total neutral lipid storage exceeds 20% of dry weight in microalgal cells are oleaginous microalgae with development potential. [0003] The neutral lipid accumulation metabolism of microalgae is accumulated under the influence of various conditioned factors in the late stage of algae proliferation. Therefore, the conditions of cell proliferation culture and neu...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N13/00C12R1/89
Inventor 周成旭严小军骆其君王建沅蒋莹文欣
Owner NINGBO UNIV
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