4 results about "Lipid storage" patented technology

The invention provides an umbilical cord mesenchymal stem cell adipogenic induced differentiation culture medium and a culture method, and belongs to the technical field of stem cell induced differentiation. Comprising a DMEM culture medium, fetal calf serum, dexamethasone, insulin, indometacin, IBMX, Zardaverine and glabridin. Zardaverine and glabridin are added into an umbilical cord mesenchymal stem cell adipogenesis induced differentiation culture medium according to a certain concentration, and the synergistic effect of Zardaverine, glabridin and other components can better induce the umbilical cord mesenchymal stem cells to differentiate into adipocytes, increase the number of lipid droplets, increase the volume of the lipid droplets, enhance the lipid storage capacity of the adipocytes, and improve the adipogenesis of the umbilical cord mesenchymal stem cells. The adipogenic differentiation induction period is shortened, and the problems of low adipogenic differentiation rate, long induction time, few and small lipid droplets, unstable adipogenic effect and the like in the three-dimensional differentiation identification of the umbilical cord mesenchymal stem cells are solved.

The present invention provides, inter alia, compositions and methods for using CB1 cannabinoid receptor agonists, or other compounds capable of increasing endocannabinoids or endocannabinoid signaling, for treating and preventing lysosomal storage disorders in which lipid storage occurs (including, e.g., disorders associated with sphingomyelin accumulation). In particular embodiments, the present invention provides compositions and methods for treating such lysosomal storage disorders with one or more fatty acid amide hydrolase inhibitor alone or in combination with one or more additional agent.

The application discloses STUB1 application of the gene or the STUB1 protein coded by the gene in the preparation of a medicine for preventing or treating obesity and related metabolic diseases STUB1 The application first proves that the gene and the coded protein are key target points for regulating fat metabolism, and the bidirectional regulation value is determined: on one hand, inhibition of STUB1 can significantly up-regulate the CIDEC protein level, promote normal fat storage of adipocytes, and improve insulin resistance and non-alcoholic fatty liver disease; on the other hand, activation of STUB1 can accelerate the degradation of CIDEC, inhibit lipid storage, realize the effect of reducing fat, and provide a brand-new intervention target point and treatment idea for metabolic diseases such as obesity, type 2 diabetes and dyslipidemia.

The present invention provides an umbilical cord mesenchymal stem cell adipogenic differentiation medium and a culture method, belonging to the technical field of stem cell induction differentiation. The medium comprises DMEM culture medium, fetal bovine serum, dexamethasone, insulin, indomethacin, IBMX, zardaverine, and glabridin. By adding zardaverine and glabridin to the umbilical cord mesenchymal stem cell adipogenic differentiation medium at a certain concentration, the synergistic effect of zardaverine, glabridin, and other components can better induce the umbilical cord mesenchymal stem cells to differentiate into adipocytes, increase the number of lipid droplets, increase the volume of lipid droplets, enhance the lipid storage capacity of adipocytes, shorten the adipogenic differentiation induction cycle, and solve the problems of low adipogenic differentiation rate, long induction time, few and small lipid droplets, and unstable adipogenic effect in the three-way differentiation identification of umbilical cord mesenchymal stem cells.