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Method for improving yield of vitamin PQQ according to fermentation method

A technology of vitamin and fermentation method, which is applied in the field of increasing the output of vitamin PQQ by fermentation method, and can solve the problems of low efficiency and low output.

Pending Publication Date: 2017-11-24
ANHUI NEW SIMON BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although Phytophagocytosis can ferment and produce PQQ, it still has the defects of low yield and low efficiency compared to production applications.

Method used

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  • Method for improving yield of vitamin PQQ according to fermentation method

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Effect test

Embodiment 1

[0087] Fermentation method improves the method for vitamin PQQ output, described method comprises the following steps:

[0088] (1) Screening the filamentous microbes: carrying out liquid culture of the filamentous microbes, taking the bacterial liquid in the logarithmic production phase, irradiating the bacterial suspension with a 30W ultraviolet lamp, the distance is 20cm, the mortality is controlled at 50%, and then diluted, Place the diluted suspension in a petri dish, place the petri dish on a stirrer 30 cm away from the UV lamp, irradiate for 30 s, then plate-coat in a sterile environment, and then in a dark environment at 35 °C Inverted and cultivated for 72h, and selected to obtain hyphae in good growth condition;

[0089] (2) plate culture: inoculate the filamentous microbes obtained in step (1) on the sterilized plate medium, cultivate at 30° C. for 7 days to obtain a single coated colony, and take 3 of the coated single colony, Each seed was seeded with 2 seeds and...

Embodiment 2

[0144] Fermentation method improves the method for vitamin PQQ output, described method comprises the following steps:

[0145] (1) Screening of the filamentous microbes: carrying out liquid culture of the filamentous microbes, taking the bacterial liquid of the logarithmic production stage, and irradiating the bacterial suspension with a 30W ultraviolet lamp, the distance is 30cm, and the mortality rate is controlled at 80%, and then diluted, Place the diluted suspension in a petri dish, place the petri dish on a stirrer 35 cm away from the UV lamp, irradiate for 180 s, then plate-coat in a sterile environment, and then in a dark environment at 35 °C Inverted and cultivated for 72h, and selected to obtain hyphae in good growth condition;

[0146] (2) plate culture: inoculate the filamentous microbes obtained in step (1) on the sterilized plate medium, cultivate at 37° C. for 8 days to obtain a single coated colony, and take 5 of the coated single colonies, Each seed was seed...

Embodiment 3

[0201] Fermentation method improves the method for vitamin PQQ output, described method comprises the following steps:

[0202] (1) Screening of the hyphae bacteria: carry out the liquid culture of the hyphae microorganisms, take the bacterial liquid in the logarithmic growth phase, centrifuge to get the supernatant, add the physiological saline, take the bacterial liquid and spread it on the slide, put it in the ARTP, and the treatment distance is 2 mm. , gas volume 10SLM, power 120W, processing time 25s, place the slide in physiological saline, shake, wash out to obtain eluate, dilute the eluate, carry out plate coating, culture, select and obtain the raw silk with good growth condition microbes;

[0203] (2) plate culture: inoculate the filamentous microbes obtained in step (1) on the sterilized plate medium, cultivate at 30° C. for 8 days to obtain a single coated colony, and take 4 of the coated single colonies, Each seed was seeded with 3 seeds and cultured on a plate m...

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Abstract

The invention provides a method for improving the yield of a vitamin PQQ according to a fermentation method. The method provided by the invention comprises the following steps: firstly, breeding hyphomicrobium with good growing state, vigorous vitality and strong metabolic capability, then allocating a culture medium and performing strain activation on the selected hyphomicrobium by the solid culture medium, in which trace element solution is added, for getting ready for selection of optimal strains; inoculating the hyphomicrobium on a specially-made shake flask seed culture medium for cultivation under appropriate conditions to enlarge the quantity of floras of the excellent hyphomicrobium, so as to obtain shake flask seed solution; then inoculating the shake flask seed solution on a specially-made seed culture medium for cultivation under appropriate conditions to the logarithmic phase, so as to obtain seed bacteria solution; finally, inoculating the seed bacteria solution on a specially-made fermentation culture medium. The fermentation culture medium provided by the invention can not only provide sufficient nutrients for the hyphomicrobium, but also inhibit undesirable growth factors, promote the hyphomicrobium to quicken the metabolism velocity and improve the yield of the vitamin PQQ; fermentation solution containing the vitamin PQQ is obtained; the fermentation yield of the hyphomicrobium is 1 g / L or higher, and the extraction rate of the hyphomicrobium is 50% or higher.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for improving the output of vitamin PQQ by a fermentation method. Background technique [0002] The scientific name of PQQ is pyrroloquinoline quinone (Pyrroloquinoline quinone for short PQQ), molecular formula: C 14 H 14 N 2 Na 2 O 8 , is a neoprosthetic group and the third prosthetic group found in membrane-bound bacterial dehydrogenases after flavin nucleotides (FMN, FAD) and nicotinamide nucleotides (MAD, NADP), The world medical community calls it the fourteenth vitamin. As a new type of water-soluble vitamin, PQQ is a prosthetic group of oxidoreductase. It is very rare and exists in some microorganisms, plants and animal tissues. It not only participates in catalyzing redox reactions in organisms, but also has some special biological activities. and physiological functions, such as shortening the lag period of Acetobacter rancens and Saccharo...

Claims

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Application Information

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IPC IPC(8): C12P17/18C12N1/20C12R1/01
CPCC12N1/20C12P17/182
Inventor 荣东褚伟立扈建斌刘博蒋栋梁
Owner ANHUI NEW SIMON BIOTECH CO LTD
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