388 results about "Vitamin a1" patented technology

The invention discloses a method for improving beef quality, an action mechanism and an experimental method, in a cattle body, vitamin A can activate the expression of EBF2 through an active metabolite RA of the vitamin A, and the EBF2 can inhibit the transcription process of CYP26B1 in a targeted manner to maintain the activity of a retinol signal channel, so that PPAR gamma and downstream lipid metabolism related genes thereof are activated, and the activity of the retinol signal channel is improved. Fatty acid transport and lipid accumulation in fat cells in the cattle muscle are promoted, fat deposition in the cattle muscle is promoted, and the beef quality is improved. By constructing the molecular network for regulating and controlling the formation of the fat in the cattle muscle, a complex regulation and control mechanism for controlling the fat deposition in the cattle muscle can be understood more deeply, a method for improving the beef quality based on regulating and controlling the vitamin A mediated key factor EBF2 is provided, and a new way is provided for improving the beef quality in a targeted manner.

The invention relates to the technical field of exosomes, in particular to an exosome compound preparation with a hair growing effect and a preparation method of the exosome compound preparation. Comprising 12 to 22 parts of ginsenoside Rb1, 15 to 12 parts of biotin tripeptide-15, 8 to 15 parts of diammonium glycyrrhizinate, 50 to 70 parts of fish collagen peptide, 10 to 20 parts of soybean peptide, 0.5 to 4.5 parts of SHH protein, 0.5 to 2.5 parts of SMO inhibitor, 0.5 to 1.5 parts of HhSP protein, 5 to 10 parts of N-acetyl tyrosine, 0.1 to 2 parts of hyaluronic acid, 0.1 to 1 part of melatonin, 0.1 to 1 part of quercetin, 0.1 to 1 part of kaempferol, 0.1 to 1 part of tanshinone, 0.1 to 1 part of biotin and 0.1 to 1 part of vitamin A. The compound preparation is combined with exosomes from various sources, so that the hair papilla cell function is synergistically enhanced, and the damaged hair follicle structure is repaired.

The invention discloses streptococcus thermophilus AK223-6 and application thereof in preparation of products for regulating intestinal tracts, improving memory and improving epilepsy, and belongs to the field of microorganisms and biological medicines. The invention provides streptococcus thermophilus AK223-6 which is preserved in the Guangdong Microbial Culture Collection Center on July 8, 2024, the preservation date of the streptococcus thermophilus AK223-6 is July 8, 2024, and the preservation number of the streptococcus thermophilus AK223-6 is GDMCCNO: 64835. Based on the streptococcus thermophilus, a metabiotic product is successfully researched and developed, and verification shows that the metabiotic product has the effects of regulating intestinal flora balance, promoting digestion and absorption of a human body, improving memory, improving epilepsy and reducing epilepsy times. After 100mg of a metabolite product containing 16,000,000,000 strains and metabolites is given every day, lactic acid bacteria polysaccharide, ergosterol, cephalin, vitamin A and vitamin B1 in the endophytic necessary quantity can be continuously provided in the intestinal reproduction and growth process, the memory and epilepsy can be effectively improved, the epilepsy frequency is reduced, and the practical application value is good.

The invention provides a multi-nutrient balanced lick brick for grazing down producing goats as well as a preparation method and application thereof, and belongs to the technical field of animal feed development. The multi-nutrient balanced lick brick is prepared from the following raw materials in parts by weight: 20 to 100 parts of copper sulfate pentahydrate, 35 to 165 parts of zinc sulfate monohydrate, 0.1 to 1 part of sodium selenite, 54.5 to 270 parts of anhydrous ferrous sulfate, 10 to 40 parts of manganese sulfate monohydrate, 15 to 65 parts of 1 percent cobalt chloride, 0.2 to 2 parts of potassium iodide, 40 to 200 parts of DL-alpha-tocopheryl acetate, 0.2 to 2 parts of vitamin A acetate, 40 to 200 parts of calcium carbonate and 20 to 100 parts of adhesive. And 8855-9765 parts of sodium chloride. The proportion of various nutritional ingredients is reasonably adjusted, the requirements of the grazing cashmere goats for microelements and vitamins can be comprehensively met, then the cashmere density is increased, and the cashmere yield is increased. Meanwhile, the fineness of cashmere can be reduced, and the quality of cashmere is improved.

The invention discloses an antioxidant vitamin A ester microcapsule and a preparation method thereof, vitamin A ester is prepared into compact solid powder, external oxygen is effectively isolated, the solid powder is used as a core material, one or two of hydrogenated oil or monoglyceride is / are mixed with phospholipid as a wall material, and the antioxidant vitamin A ester microcapsule is prepared. The core material and the wall material are mixed and then subjected to high-speed dispersion and spray condensation treatment to obtain the vitamin A ester microcapsule, so that the storage stability of the vitamin A ester is greatly improved; meanwhile, the preparation process is simple, and large-scale production is facilitated. The encapsulation efficiency of the antioxidant vitamin A ester microcapsule prepared by the method is more than 90%. And in 35-day accelerated storage test evaluation at 37 DEG C, the retention rate is 80% or above and is obviously higher than the retention rate of the raw materials.

Methods are described for measuring the amount of one or more of vitamin A, α-tocopherol, and the combination of β-tocopherol and γ-tocopherol in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying one or more of vitamin A, α-tocopherol, and the combination of β-tocopherol and γ-tocopherol in a sample.

Provided is a compound of formula I, X-Y (I) wherein: Y comprises a peptide fragment of formula IV, [Ala] m-[Lys-V]n-Lys-V (IV) in which m represents 0 or 1, n represents an integer 0, 1, 2, 3 or 4 and, each V independently represents a 2 to 4 amino acids sequence, in which the amino acids are selected from one or more of the group Lys, Pro, Hyp, diHyp, Thr, pThr, DOPA, Tyr, and Ser; and X represents at least one optional substituent selected from: (i)a lipid, which lipid is selected from the group consisting of vitamin A, vitamin E, cholesterol and a fatty acid; and / or (ii)a non-steroidal anti-inflammatory drug, which compounds are useful in medicine, including as pharmaceutical excipients, adhesives and film-forming materials and / or are useful in the treatment of conditions characterised by inflammation, including wounds, burns, and disorders of the mucosa, such as skin diseases, oral diseases, gynecological diseases and IBDs.

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.

The invention discloses a sheep colostrum composite powder composition capable of improving immunity and a preparation method of the sheep colostrum composite powder composition, and belongs to the technical field of milk composite powder. Comprising the following components in parts by weight: 15 to 20 parts of compound microcapsule probiotics, 200 to 300 parts of sheep colostrum dry powder, 20 to 25 parts of dissolution promoting polypeptide, 0.005 to 0.009 part of vitamin A, 0.003 to 0.006 part of vitamin D, 0.008 to 0.01 part of ascorbic acid, 0.003 to 0.005 part of vitamin K1, 0.006 to 0.009 part of vitamin B2 and 0.002 to 0.004 part of folic acid. According to the preparation method, the technical effects that the dissolution speed is increased, and caking and precipitation are not prone to occurring are achieved in a mode of preparing the dissolution-promoting polypeptide.

The invention discloses vitamin A inclusion powder as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. The vitamin A inclusion powder comprises the following raw materials: a vitamin A active component, a capsule material, a filler, a stabilizer, a pellet core and water, the capsule material comprises Arabic gum and / or gelatin; the filling agent comprises cane sugar and maltodextrin; the preparation method of the vitamin A inclusion powder comprises the following steps: dissolving a capsule material in water to prepare a water phase; mixing the vitamin A active ingredient with the stabilizer to prepare an oil phase; mixing the water phase with the oil phase to prepare a primary emulsion; homogenizing the primary emulsion, adding a filling agent, and mixing to prepare a spray solution; performing fluidized bed spray drying on the spray solution to pellet cores to prepare the vitamin A inclusion powder; the atomization pressure of the spray drying of the fluidized bed is 2.0-4.0 bar. The vitamin A inclusion powder prepared by the invention is stable in quality, low in adhesion degree and good in long-term stability.

The invention relates to application of 9-cis-retinoic acid in preparation of products for treating, preventing or improving obesity, and belongs to the technical field of biological medicine. Experimental evaluation of obese mice induced by high-fat diet proves that the 9-cis-retinoic acid can reduce blood fat and improve the glycometabolism function, and lipid accumulation of the liver is improved mainly by increasing expression of main regulatory protein PPAR-alpha oxidized by liver beta, so that the 9-cis-retinoic acid can be used for preparing medicines for treating obesity. The traditional Chinese medicine composition can be used for treating, preventing or improving metabolic symptoms caused by high fat diet, and a new strategy is provided for treating obesity and related metabolic syndromes. Besides, 9-cis-retinoic acid is a natural active metabolite of vitamin A, does not show obvious toxic reaction in animal experiments, and has better biocompatibility.

The invention discloses a method for detecting the content of fat-soluble vitamins A, D and E in blood. The method comprises the following steps: adding 250 mu L of BHT (butylated hydroxytoluene) internal standard solution into every 50 mu L of a blood sample; adding 20 [mu] L of a zinc sulfate heptahydrate solution into the blood sample added with the BHT internal standard solution, and sequentially carrying out primary uniform mixing and centrifugal treatment; taking 100 microliters of centrifuged supernate, and concentrating and drying; adding 50 [mu] L of a PTAD (4-phenyl-1, 2, 4-triazoline-3, 5-diketone) acetonitrile solution into the concentrated and dried supernatant at normal temperature, carrying out a derivatization reaction, and then adding a quenching agent to terminate the derivatization reaction so as to obtain a derivative sample; and adding 100 microliters of centrifuged supernate into the derivative sample, uniformly mixing again, and detecting. Through screening of the quenching agent, the response of the vitamins can be obviously enhanced, and the detection of the contents of the fat-soluble vitamins A, D and E can be rapidly and accurately completed.

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.

The present disclosure relates to the field of livestock farming, and specifically provides a feeding method for gilts in batch for promoting onset of puberty and alleviating estrus disorder of gilts, which is aiming at an industrial problem that a large number of gilts are culled due to delayed puberty onset or estrus disorder. The method includes: feeding, prior to onset of puberty for gilts with vitamin A combined with lactic acid; and performing, subsequent to the puberty onset of gilts, estrus synchronization treatment and insemination treatment on the gilts. By applying vitamin A combined with lactic acid, the present disclosure alleviates the delayed puberty onset or estrus disorder of the gilts, reduces the feeding duration and feeding cost of the gilts, facilitates synchronous estrus treatment of the gilts.

The present invention belongs to the technical field of feed preparation, and specifically relates to a method for restoring the egg production of parent pigeons after a typhoon disaster in Hainan. The present invention prepares parent pigeon feed by mixing corn, quinoa, cricket powder, Scenedesmus, almonds, shell powder, salt, vitamin A and vitamin E, and adds a composite bacterial agent to the parent pigeon feed, which is beneficial to the remodeling of the intestinal microbial flora of the parent pigeons, maintaining their metabolism, and enhancing their immunity. Therefore, the above substances are fed to parent pigeons after the disaster, and Chinese medicine extracts are added to the drinking water of the parent pigeons after the disaster for feeding. The egg production of parent pigeons after the typhoon disaster can be significantly restored, the morbidity of parent pigeons after the disaster is reduced, and a scientific basis is provided for restoring the production performance of parent pigeons after the typhoon.

The invention belongs to the technical field of functional food, and particularly relates to a nutritional supplement composition containing various vitamins and mineral substances. The nutritional supplement composition comprises the following components: black pure fat chocolate, vitamin A, vitamin D3, vitamin E, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, folic acid, nicotinamide, biotin, calcium pantothenate, heavy magnesium carbonate, ferrous fumarate, zinc gluconate, sodium selenite, copper sulfate, potassium iodide, manganese gluconate and calcium carbonate. The preparation method comprises the following steps: preparing a powder premix; then adding the powder premix into the molten black pure fat chocolate and stirring; the materials are prepared into an inner core containing vitamins and mineral substances through a chocolate forming machine, and then the inner core is coated. The multivitamin mineral sugared pill product provided by the invention is good in taste and stable in effective components, and can effectively relieve fatigue, promote physical and psychological health, improve sleep and improve memory.

The invention discloses a qualitative and quantitative detection method for vitamin A and vitamin E in food. The qualitative and quantitative detection method comprises the following steps: step 1, pre-treating a sample and accurately adding an isotope internal standard: adding d6-alpha-tocopheryl acetate and d8-retinol acetate into the sample before saponification and preparing a blank matrix; 2, pre-treatment parameter QbD-DOE collaborative optimization, wherein a response surface method is adopted for conducting center composite design on the saponification temperature, time and BHT concentration, and the interaction effect of all the factors is analyzed to determine an optimal operation window; step 3, intelligently optimizing chromatography-mass spectrometry conditions and tuning parameters; step 4, constructing a matrix matching calibration curve and performing WLS quantitative analysis: performing gradient dilution on a standard substance by utilizing a blank matrix extracting solution, adding an isotope internal standard to prepare a 5-7-point matrix matching calibration curve, fitting the curve by adopting a weighted least square method, and verifying the correlation and intercept change of the calibration curve every day; and 5, multi-dimensional qualitative confirmation and intelligent diagnosis system operation.

The invention discloses a nutritional food capable of improving a night vision function and a preparation method of the nutritional food. The composition is prepared from anthocyanin-sterculia nobilis acid nano-particles, fucoxanthin-vitamin A-resveratrol nano-particles and zinc taurate. The functions of retinal rod cells can be remarkably improved, the blood microcirculation of eyes can be promoted, and the vision sensitivity and the asthenopia resistance in dark environments such as night, weak light and low visibility can be remarkably improved; the product can be prepared into capsules, tablets, electuary, oral liquid or beverage.

The invention provides a method for reducing yellow chromaticity of vitamin A acetate. The method comprises the following steps: decoloring a vitamin A acetate crude product and activated carbon in the presence of a solvent, filtering the activated carbon, and removing the solvent from filtrate to obtain the vitamin A acetate with obviously reduced color. By controlling the key indexes of the activated carbon, the VA loss rate in the activated carbon decoloration process is lower than 5%, and the problem of low yield in the VA decoloration process is effectively solved.

The invention discloses ointment for preventing infantile hand-foot-and-mouth disease and a preparation method thereof. The ointment is prepared from coptis chinensis, sweet wormwood, honeysuckle, radix sophorae flavescentis, lithospermum, sanguisorba officinalis, radix isatidis, menthol, clove oil, vitamin A oil, vitamin E oil, roxburgh rose seed oil, olive oil, chamomile extract, honey and microcrystalline wax. On the basis of combination of traditional Chinese medicines and western medicines, traditional Chinese medicine components such as coptis chinensis, artemisia apiacea and honeysuckle and western medicine components such as vitamin A and vitamin E are combined, and the ointment for preventing the infantile hand-foot-and-mouth disease is prepared. The components have the effects of clearing away heat and toxic materials, resisting viruses, bacteria and inflammation and the like, and are beneficial to enhancing the immunity of the organism, inhibiting virus diffusion and relieving disease symptoms, so that the occurrence of the infantile hand-foot-and-mouth disease is effectively prevented.

The invention relates to the technical field of preparation of health-care products, in particular to a fat-soluble component nano-emulsion and a preparation method thereof. The preparation method comprises the following steps: stirring and dissolving the antioxidant and the caprylic / capric triglyceride, adding the vitamin D3 and the retinyl palmitate, and stirring and dissolving to obtain an oil phase; stirring the starch sodium octenylsuccinate in purified water until the starch sodium octenylsuccinate is dissolved, adding glycerol, and uniformly stirring and mixing to obtain a water phase; adding a small amount of water phase into the oil phase, and stirring at high speed to obtain primary emulsion; adding the primary emulsion into the residual water phase, stirring at a high speed, shearing and homogenizing to obtain a nano embedding emulsion; adding citric acid, cane sugar and sodium ascorbate into purified water, adding the nano embedding emulsion, and uniformly stirring to obtain a concentrated solution; and diluting the concentrated solution and adjusting the pH value to obtain the fat-soluble component nano-emulsion. The fat-soluble component nano-emulsion provided by the invention has good safety; the product can be easily absorbed and utilized by a human body, and is convenient for people to quickly supplement vitamin A and vitamin D3 required by the body.

The invention relates to the field of detection, in particular to a method for simultaneously detecting multiple fat-soluble vitamins, which comprises the following steps: (S.1) carrying out protein precipitation and liquid-liquid extraction on a sample to be detected to obtain an extract containing fat-soluble vitamins; (S.2) carrying out derivatization reaction on the extract by using a PTAD derivatization reagent; and (S.3) analyzing the derivatized product by adopting a liquid chromatography-tandem mass spectrometry method, wherein the detected target fat-soluble vitamins comprise vitamin A, vitamin E, 25-hydroxy vitamin D2, 25-hydroxy vitamin D3, vitamin K1, vitamin K2 and vitamin K2. According to the application, PTAD is creatively adopted as a unified derivatization reagent, simultaneous and efficient derivatization of seven fat-soluble vitamins is successfully realized, the detection sensitivity of low-content targets, especially vitamin D, K2 and the like, is greatly improved, and the core problems of weak signal response and difficulty in accurate quantification are solved.

The present invention provides a vitamin premix and a preparation process thereof, and belongs to the technical field of food additives, the preparation process is as follows: fat-soluble vitamin mixed particles, mixed water-soluble vitamins and mixed minerals are added into a mixer for mixing, then vitamin C, magnesium taurate, amino acids and tricalcium phosphate are added for continuous mixing, and sieving is performed to obtain the vitamin premix. Wherein the fat-soluble vitamin mixed particles comprise the following raw materials: vitamin A, vitamin E acetate, vitamin D3, vitamin K2, Arabic gum, modified protein and beta-cyclodextrin; the mixed water-soluble vitamins comprise the following raw materials: vitamin B1, vitamin B2, vitamin B5, vitamin B12, folic acid and nicotinamide; the mixed mineral substance comprises the following raw materials: ferrous glycinate, calcium citrate, magnesium citrate and zinc glycinate. The vitamin premix prepared by the invention can be used as a nutritional supplement in various foods, is convenient to use, and has good stability and mixing uniformity.

The invention discloses a resource utilization method of low-quality C15 phosphonium salt. The preparation method comprises the following steps: (1) performing thermal decomposition reaction and isomerization reaction on the low-quality phosphonium salt to generate the C15 phosphonium salt; (2) adding a solvent into the reaction liquid obtained in the step (1) for extraction and phase separation, and collecting a product phase containing a C15 phosphine salt isomer; and (3) adding pentacaraldehyde and carbonate into the product phase containing the phosphonium salt, and carrying out condensation reaction to prepare the vitamin A acetate. According to the method, the low-quality phosphonium salt generated in the C15 phosphonium salt production process can be recycled, the produced low-grade vitamin A acetate is sold, the product mark is enriched, and the industrial competitiveness of the product is improved. The process is simple to operate, mild in reaction condition, green and environment-friendly, free of waste liquid and suitable for industrial production, and the industrial chain cost of the vitamin A acetate is greatly reduced.

The invention discloses a production process of anti-hair loss baked grain, which relates to the technical field of baked grain production, comprising 65% chicken, 20% salmon meat, 3% chicken liver, 3% chicken heart, 2% deep-sea fish oil, 2% Antarctic krill oil, brewer's yeast powder, 1% chicken fat, mixed fruit and vegetable powder, cellulose, yucca powder; additive composition: 0.3% manno-oligosaccharide, 0.3% xylo-oligosaccharide, egg yolk lecithin, calcium hydrogen phosphate, serine, sodium hyaluronate for pets, taurine, lysine, methionine, tyrosine, glutamine, chloride Choline, vitamin A, vitamin D3, vitamin E, niacinamide, D-calcium pantothenate, vitamin B1, vitamin B2, vitamin B6, vitamin B12, folic acid, biotin, protein zinc, protein iron, protein copper, protein manganese, rosemary extract, tea polyphenols, S1, raw and auxiliary materials collection: S2, meat paste enzymatic hydrolysis, add the minced material into the enzymatic hydrolysis tank, the enzymatic hydrolysis time is one hour, the temperature is 50°-60°, after reaching the specified time, the temperature is raised to inactivate the enzyme, and the material is discharged at the lower temperature. The present invention has the characteristics of high process integration.

The purpose of the present invention is to provide an oil-in-water type emulsion cosmetic which is capable of stably holding a vitamin A fatty acid ester even if both the vitamin A fatty acid ester and an ultraviolet absorber are blended, and which has excellent emulsion stability. The oil-in-water emulsion cosmetic according to the present invention comprises (A) a vitamin A fatty acid ester, (B) an ultraviolet absorber, (C) a nonionic surfactant, and (D) a hydrophilic thickener, and is characterized in that: the content of the vitamin A fatty acid ester (A) is 0.001-0.5 mass% relative to the total amount of the cosmetic; the ratio of the content of the nonionic surfactant (C) to the content of the hydrophilic thickener (D) is in the range of 0.4-0.9.

The present application relates to the technical field of cosmetic raw materials, and provides tocopheryl retinoate and application thereof in cosmetics and / or skin care products. The tocopheryl retinoate introduces a tocopherol group with antioxidant activity into the molecular structure of retinoic acid, significantly improves the light stability and thermal stability of the compound while maintaining the biological activity of vitamin A ingredients, and effectively reduces the irritation to the skin. The tocopheryl retinoate is suitable for the cosmetic and / or skin care product system used in the daytime, can improve the skin condition, repair the skin barrier and protect the skin, and has good safety and wide application prospect.

The invention relates to the technical field of vitamin A micro-capsule production, in particular to a vitamin A micro-capsule and a preparation method thereof. Research finds that the roundness of the vitamin A micro-capsule prepared by the emulsion through a spray drying process can be controlled by controlling the size of a relational expression 0.88 * ln (Q) + 0.12 * e (-0.002 mu) * (N / 1000) 0.18-0.05 * Te-120 + 0.1 * (epsilon / 2.4) 0.5, the relational expression obtained through parameter calculation of the spray drying process is controlled to be 50% or above, the vitamin A micro-capsule with the roundness being 50% or above can be obtained, and the vitamin A micro-capsule with the roundness being 50% or above can be used for preparing the vitamin A micro-capsule with the roundness being 50% or above. The roundness of the vitamin A micro-capsule is remarkably improved, so that the situation that the micro-capsule is released too early in gastric juice is improved, the intestinal targeting property is improved, meanwhile, the situation that the micro-capsule is degraded in the storage period is improved, and the stability of the micro-capsule is improved.

The application discloses a method for promoting transport and improving retinal synthesis, which is obtained by introducing an exogenous ABC transport protein Pdr5 into a host fungus and adding a copy of beta-carotene-15, 15'-monooxygenase BLH; wherein the host fungus is a genetically modified Pichia pastoris strain PP-BCCIV. The application further verifies the retinal production performance of the recombinant strain by screening different surfactants, and further improves the ability of Pichia pastoris to produce retinal. At the same time, all the retinal is extracted into the extracellular, and all the carotenes are left in the intracellular, so as to achieve the co-production of carotenes and retinal. The recombinant Pichia pastoris of the application has a simple construction method, can better promote the synthesis of retinal and other vitamin A, and can make the engineering strain utilize glucose and methanol to efficiently synthesize retinal through a 5 L fermentation tank, which is beneficial to industrial production.

The invention provides a preparation method of a cholestatic liver injury animal model, which comprises the following steps: on the basis that a mouse is fed with a feed containing 500IU / kg vitamin A for 8 weeks to cause deficiency of vitamin A in the mouse body, performing intragastric administration of alpha-naphthyl isothiocyanate (ANIT) at 50mg / kg for 4 weeks once a week, so as to obtain the cholestatic liver injury mouse model. The blood biochemical index ALT, the liver index, the pathological structure change of the liver tissue and the bile acid deposition of the liver tissue are more obvious. The cholestatic liver injury model prepared by the preparation method can avoid the influence of inconsistent dosage and unstable model in a conventional method, and can be applied to the research fields of molecular mechanism research of occurrence and development of cholestatic liver injury, finding of cholestatic liver injury treatment medicines and methods and the like.