Compound vitamin composition for improving pet hair quality and pet feed

Abstract

The invention discloses a composite vitamin composition for improving pet hair quality and pet feed, and each kilogram of feed comprises the following components: 6000 IU to 12000 IU of vitamin A; 20 mg to 80 mg of vitamin E; 4 mg to 8 mg of vitamin B2; 10 mg to 30 mg of vitamin B5; 0.4 mg to 0.8 mg of vitamin B7; and the content of vitamin C is 100 to 200 mg. According to the technical scheme, all the components have a synergistic effect, so that pet hair is vigorous and glossy, and the problems of hair embrittlement, dryness and the like are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of animal nutrition, and in particular to a composite vitamin composition and pet feed for improving pet hair quality. Background Art

[0002] In recent years, pet ownership has become increasingly popular in China, with their status continuously rising, making them an indispensable member of many families. The health of a pet's coat not only impacts its appearance but also directly impacts its owner's care experience. As the aging of the pet population intensifies, skin health and coat quality issues are becoming increasingly prominent in older dogs and cats.

[0003] Free radical theory posits that as animals age, their ability to scavenge free radicals declines. Excessive free radical accumulation can lead to cellular aging and damage, including cyst cells. Over 60% of pets over 10 years old exhibit aging characteristics such as dry hair, reduced luster, and weakened hair shaft structure. Studies have shown that hair degeneration is closely associated with a decline in epidermal barrier function and reduced hair follicle stem cell activity in older individuals. Vitamins are key regulators of hair follicle cell differentiation, keratin synthesis, and repair of oxidative damage. Vitamin A maintains hair health and luster by regulating the differentiation of hair follicle keratinocytes and lipid secretion in the sebaceous glands. Vitamin E, as an antioxidant, protects the structural integrity of hair follicle cell membranes by scavenging free radicals and inhibiting lipid peroxidation. However, serum concentrations of both vitamins are lower in older animals than in younger individuals due to decreased intestinal absorption efficiency and impaired enterohepatic circulation.

[0004] Conventional pet nutritional supplements often use a simple addition model of a single vitamin (such as biotin or vitamin E), but ignore the lack of vitamin synergistic effects. For example, vitamin A deficiency will lead to abnormal hair follicle keratinization, while excessive supplementation can cause hypercalcemia and hepatotoxicity due to dosage overload; while vitamin E alone can alleviate oxidative damage, it cannot activate the retinoic acid signaling pathway necessary for hair follicle regeneration and thus improve pet hair quality. In summary, by adding optimized vitamin ratios and synergistic ingredient combinations to feed, pet hair quality can be significantly improved while ensuring the physical health of the pet. Summary of the Invention

[0005] The technical solution of the present invention to solve the above technical problems is to provide a multivitamin composition for improving the quality of pet hair, which comprises the following components per kilogram of feed:

[0006] Vitamin A: 6000IU-12000IU;

[0007] Vitamin E: 20mg-80mg;

[0008] Vitamin B2: 4mg-8mg;

[0009] Pantothenic acid: 10 mg - 30 mg;

[0010] Biotin: 0.4 mg - 0.8 mg;

[0011] Ascorbic acid: 100 mg - 200 mg.

[0012] Furthermore, per kilogram of feed, it contains Omega-3 fatty acids: 0.5 g–2 g.

[0013] Furthermore, per kilogram of feed, the content of each component is:

[0014] Vitamin A: 9000 IU;

[0015] Vitamin E: 40 mg;

[0016] Riboflavin: 6 mg;

[0017] Pantothenic acid: 20 mg;

[0018] Biotin: 0.6 mg;

[0019] Ascorbic acid: 150 mg.

[0020] Furthermore, per kilogram of feed, the content of the said Omega-3 fatty acids is 1 g.

[0021] Furthermore, per kilogram of feed, it contains cholecalciferol: 200–400 IU.

[0022] To solve the above technical problems, the present invention also provides a pet feed, which contains the above-mentioned compound vitamin composition for improving the hair quality of pets.

[0023] The technical solution of the present invention includes the following vitamin components: vitamin A, vitamin E; riboflavin; pantothenic acid; biotin; ascorbic acid; Omega-3 fatty acids. All components work together to make the pet's hair lush, shiny, and relieve problems such as brittle and dry hair. The present invention designs vitamin A+E: regulating the secretion of sebaceous glands, enhancing the epidermal barrier function, and reducing hair dryness and cracking; riboflavin + pantothenic acid + biotin: accelerating the metabolism of hair follicle cells, promoting keratin synthesis, and improving hair toughness; ascorbic acid: inhibiting oxidative stress, reducing hair follicle inflammation, and prolonging the hair growth period. Omega-3: enhancing the closure of hair scales and improving hair gloss. Description of the Drawings

[0024] To more clearly illustrate the technical solutions in the embodiments of the present invention or in the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on the structures shown in these drawings.

[0025] Figure 1 Photographs of the hair of mice with different scores;

[0026] Figure 2 Graph showing the effect of vitamins with different formulations on the hair of mice;

[0027] Figure 3 Graph showing the effect of vitamins and co-lipids with different formulations on the hair of mice. Detailed implementation manners

[0028] The present invention provides a compound vitamin composition and pet feed for improving the hair quality of pets, aiming to design a compound vitamin composition for improving the hair quality of pets.

[0029] The following will illustrate the compound vitamin composition for improving the hair quality of pets proposed by the present invention in specific embodiments:

[0030] In the technical solution of this embodiment, as Figure 1 shown, a compound vitamin composition for improving the hair quality of pets, per kilogram of feed, comprises the following components:

[0031] Vitamin A: 6000 IU - 12000 IU;

[0032] Vitamin E: 20 mg - 80 mg;

[0033] Vitamin B2: 4 mg - 8 mg;

[0034] Vitamin B5: 10 mg - 30 mg;

[0035] Vitamin B7: 0.4 mg - 0.8 mg;

[0036] Vitamin C: 100 mg - 200 mg.

[0037] Further, per kilogram of feed, it comprises Omega-3 fatty acids: 0.5 g - 2 g.

[0038] Further, per kilogram of feed, the content of each component is:

[0039] Vitamin A: 9000 IU;

[0040] Vitamin E: 40 mg;

[0041] Vitamin B2: 6 mg;

[0042] Vitamin B5: 20 mg;

[0043] Vitamin B7: 0.6 mg;

[0044] Vitamin C: 150 mg.

[0045] Furthermore, based on per kilogram of feed, the content of said Omega-3 fatty acid is 1 g.

[0046] Furthermore, based on per kilogram of feed, it contains Vitamin D3: 200–400 IU.

[0047] To solve the above technical problems, the present invention also provides a pet feed, which contains the above-mentioned compound vitamin composition for improving the hair quality of pets.

[0048] Example 1:

[0049] Vitamin A: 9000 IU;

[0050] Vitamin E: 40 mg;

[0051] Vitamin B2: 6 mg;

[0052] Vitamin B5: 20 mg;

[0053] Vitamin B7: 0.6 mg;

[0054] Vitamin C: 150 mg;

[0055] Omega-3 fatty acid: 1 g.

[0056] Example 2:

[0057] A compound vitamin composition for improving the hair quality of pets, based on per kilogram of feed, contains the following components:

[0058] Vitamin A: 9000 IU;

[0059] Vitamin E: 40 mg;

[0060] Vitamin B2: 6 mg;

[0061] Vitamin B5: 20 mg;

[0062] Vitamin B7: 0.6 - 1.0 mg;

[0063] Vitamin C: 150 mg;

[0064] Omega-3 Fatty Acids: 1.2 - 2.0 g.

[0065] For long-haired pets.

[0066] Example 3:

[0067] A compound vitamin composition for improving the hair quality of pets, per kilogram of feed, contains the following components:

[0068] Vitamin A: 9000 IU;

[0069] Vitamin E: 40 mg;

[0070] Vitamin B2: 6 mg;

[0071] Vitamin B5: 20 mg;

[0072] Vitamin B7: 0.6 - 1.0 mg;

[0073] Vitamin C: 150 mg;

[0074] Omega-3 Fatty Acids: 1.2 - 2.0 g;

[0075] Vitamin D: 200–400 IU.

[0076] For senior pets.

[0077] Verification experiment:

[0078] 1. Effects of different antibiotic combinations on antioxidant activity

[0079] The free radical theory holds that if a large amount of free radicals accumulate, it will cause cell damage and then lead to aging. In senior pets, due to the decline in the body's ability to purify free radicals, in addition to the adverse effects of excessive accumulation of free radicals such as phospholipid damage and telomere shortening, it also causes the aging of hair follicle cells and subcutaneous inflammation. Since vitamins have antioxidant ability, it is considered to improve the antioxidant ability of pets by adding vitamins, thereby improving the hair quality. Given that there are currently various vitamins, the antioxidant abilities of common vitamins are detected to screen out vitamins with higher antioxidant ability.

[0080] Determination of antioxidant ability by DPPH method: Dilute the sample to be tested with ethanol in a 10-fold gradient (0.001 - 10 mg / mL), add 50 μL of the diluted sample to 950 μL of DPPH-ethanol solution (2.3%). Set up a blank group without adding the sample and a control group with only the sample. Each sample is repeated three times. After stirring the mixture, place it in the dark at room temperature for 30 minutes, and then measure the absorbance of the mixture at 517 nm.

[0081]

[0082] Where Ai, Aj, and A0 are the absorbances of the test group, blank group, and control group, respectively.

[0083] Determination of antioxidant capacity by ABTS method: Dilute the test sample 10-fold gradient with ethanol (0.001 - 10 mg / mL), add 30 μL of the diluted test sample to 300 μL of ABTS solution (diluted with ethanol to OD 734 about 0.7), incubate the reaction mixture at room temperature (25 °C) for 6 minutes, and then measure the absorbance at 734 nm. Set up the ABTS solution without sample and the control group. All measurements are repeated three times.

[0084]

[0085] Where Ai, Aj, and A0 are the absorbances of the test group, blank group, and control group, respectively.

[0086] Prepare different kinds of antibiotics (A, E, C, B2, B5, B7, K1, D3, B1, B3, B6, B9) at 0.1 mg / ml and measure their antioxidant activities. The results are as follows: Vitamins A, C, and E have high antioxidant capacities. In addition, vitamins B2, B5, and B7 also have certain antioxidant capacities, while vitamins K1, D3, B1, B3, B6, and B9 have low antioxidant capacities and are not suitable for use in pet feed.

[0087] Table 1 Determination of antioxidant capacity of single vitamins

[0088] Group DPPH Scavenging Rate (%) ABTS Scavenging Rate (%) Vitamin A 18.2±1.8 20.5±2.1 Vitamin E 35.6±2.4 38.7±3.0 Vitamin C 40.1±3.2 45.3±2.9 Vitamin B2 12.3±1.5 15.8±1.7 Vitamin B5 10.7±1.2 13.4±1.5 Vitamin B7 9.8±0.9 11.2±1.3 Vitamin K1 5.1±0.6 6.3±0.8 Vitamin D3 4.9±0.5 4.9±0.5 Vitamin B1 7.2±0.7 8.5±0.9 Vitamin B3 8.4±0.8 9.7±1.0 Vitamin B6 6.5±0.6 7.9±0.8 Vitamin B9 5.8±0.5 6.9±0.7

[0089] Vitamin A can maintain the skin barrier and support the hair growth cycle. However, due to excessive free radicals in the body environment hindering hair growth, vitamin A is difficult to play a role in an oxidative stress environment. Considering that other vitamins such as vitamin C and E have antioxidant activities, they can be paired with vitamin A to reduce the free radical level and repair hair follicles at the same time.

[0090] To verify the effect of different antibiotic combinations on antioxidant activity, design different vitamin combinations, and finally screen out the combination with the highest antioxidant activity for subsequent design.

[0091] Table 2 Compound vitamin table

[0092] Group Vitamin Combination (Each vitamin content is 0.1 mg / ml) Group 1 Vitamin A + C Group 2 Vitamin A + E Group 3 Vitamin A + E + C Group 4 Vitamin B2 + B5 + B7 Group 5 Vitamin A + E + C + B2 + B5 + B7

[0093] The ABTS and DPPH results are as follows. The clearance rate of Group 5 is significantly higher than that of other groups, proving that the vitamin synergy can maximize the antioxidant activity. For Group 1 (A + C), Group 2 (A + E), and Group 3 (A + E + C), compared with single vitamin C or vitamin E, the results show that the single combination has limited effects and lacks synergistic enhancement. When comparing the results of Group 5 (K1 + D3) with the combined results of Group 3 and Group 4, the antioxidant capacity is enhanced, indicating that there is a certain synergistic effect after mixing the six vitamins.

[0094] Table 3 Determination of the Antioxidant Capacity of Compound Vitamins

[0095] Group DPPH Scavenging Rate (%) ABTS Scavenging Rate (%) Group 1 38.5±2.1 42.3±3.0 Group 2 45.7±3.5 50.1±2.8 Group 3 52.4±1.6 55.2±2.1 Group 4 17.9±2.3 16.7±1.9 Group 5 78.3±4.2 73.6±3.8

[0096] 2. Formulation ratios of vitamin groups with different ratios

[0097] After calculation, the appropriate addition amount of vitamin A in pet feed is 5000 IU / kg (dry weight). Excessive addition can lead to hypercalcemia and hepatotoxicity in pets; while vitamin E can be detected in the serum of pet dogs, and there is no obvious improvement in the total antioxidant capacity of the body. The addition range in the feed is 50 - 400 mg / kg. Therefore, the content of single vitamins is determined according to the addition doses specified by the standards of the National Research Council (NRC) of the United States.

[0098] Table 4: Different content compositions of vitamin A and vitamin E in pet feed

[0099]

[0100] 3. Effect test of adding different doses of vitamins on hair improvement

[0101] Thirty female BALB / c mice over the age of 10 months were selected and the different formulas in 2 were added to their feed respectively. After continuous feeding for 2 months, the hair quality of the mice was scored. The scoring criteria are shown in Table 5 and Figure 1 .

[0102] Table 5: Scoring criteria for the hair status of mice

[0103]

[0104] According to the scoring results of the mice's hair, see Figure 2 , it can be seen that with the addition of vitamins, the hair quality of the mice becomes higher, and the score of Formula C is the lowest. However, as the vitamin content increases, the hair quality of the mice begins to decrease, indicating that high concentrations of vitamins start to have an inhibitory effect on the hair quality of the mice.

[0105] 4. Effect test of adding different other components on hair improvement

[0106] Omega-fatty acids or trace elements have a certain promoting effect on the hair of pets. Further, vitamins are formulated according to Formula 3 and mixed with Omega-3 fatty acids or linoleic acid respectively to explore their effects on the hair of mice.

[0107] Thirty-five female BALB / c mice over 10 months old were selected, and different formulas in Table 6 were added to their feed respectively and fed continuously for 2 months. The hair quality of the mice was scored, and the scoring results are shown in Figure 3 . According to the results, the addition of linoleic acid did not further improve the hair quality of the mice, while the addition of Omega-3 fatty acids improved the hair quality of the mice, and the high, medium and low doses all had a promoting effect.

[0108] Table 6: Different content compositions of vitamin A and vitamin E in pet feed

[0109]

[0110] As mentioned above, it is only the preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by those skilled in the art within the technical scope disclosed by the present invention should be covered by the protection scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope of the claims.

Claims

1. A compound vitamin composition for improving the hair quality of pets, characterized in that, Per kilogram of feed, it contains the following components: Vitamin A: 6,000 IU - 12,000 IU; Vitamin E: 20 mg - 80 mg; Vitamin B2: 4 mg - 8 mg; Vitamin B5: 10 mg - 30 mg; Vitamin B7: 0.4 mg - 0.8 mg; Vitamin C: 100 mg - 200 mg.

2. The compound vitamin composition according to claim 1, characterized in that, Per kilogram of feed, it contains Omega-3 fatty acids: 0.5 g–2 g.

3. The compound vitamin composition according to claim 1 or 2, characterized in that, Per kilogram of feed, the content of each component is: Vitamin A: 9,000 IU; Vitamin E: 40 mg; Vitamin B2: 6 mg; Vitamin B5: 20 mg; Vitamin B7: 0.6 mg; Vitamin C: 150 mg.

4. The multivitamin composition according to claim 2, wherein Per kilogram of feed, the content of the said Omega-3 fatty acids is 1 g.

5. The multivitamin composition according to claim 1 or 2, characterized in that, Per kilogram of feed, it contains Vitamin D3: 200–400 IU.

6. A pet feed, characterized in that, It contains a multivitamin composition for improving the hair quality of pets as described in any one of claims 1-5.

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