Primer set, application, product and method for detecting SNP sites related to drug metabolism in children

Abstract

The invention provides a primer set, application, a product and a method for detecting SNP sites related to drug metabolism in children, and relates to the field of biotechnology. The primer set of the SNP sites related to drug metabolism in children provided by the invention can be used to detect the metabolism capacity of thermoanalgesics, metabolism capacity of respiratory system drugs, metabolism capacity of neurological and psychiatric drugs, metabolism capacity of cardiovascular and cerebrovascular drugs, metabolism capacity of anti-infective drugs, metabolism capacity of endocrine drugs, metabolism capacity of digestion system drugs, metabolism capacity of anesthetic drugs and metabolism capacity of chemotherapy and immunosuppressant. Specific detection of site mutation related to common drug metabolism in children can be realized by the primer set, the accuracy is high, the detection cycle can be tremendously shortened, and the detection cost is reduced at the same time. Moreover, the needs of a drug metabolism can be predicted by the detection results, the metabolism capacity of a variety of drugs can also be comprehensively evaluated, and scientific reference is providedfor the individualized medication of children.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer set, application, product and method for detecting SNP sites related to children's drug metabolism. Background technique [0002] Globally, about one-third of the deaths of patients each year are related to irrational drug use. In my country, about 2 million people are hospitalized due to adverse drug reactions every year, and about 200,000 people die due to adverse drug reactions. Children account for 1 / 3 of the deaths due to adverse drug reactions. Irrational drug use and adverse drug reactions caused by other reasons have become the fourth leading cause of death after cancer, cerebrovascular disease and heart disease. Children are in the growth and development stage, and the physiological functions of tissues and organs have not yet fully developed, so they have special reactions to drugs. Compared with adults, children's drug use has more potential safety hazards and n...

AI Technical Summary

Problems solved by technology

[0004] At present, there are many detection methods with their own characteristics. PCR-based detection methods, such as ARMS-PCR, fluorescent quantitative PCR, digital PCR, etc., are easy to operate, fast, and simple in data analysis. However, due to the limitation of throughput, they cannot meet clinical requirements. The need for multiple genes and multiple locus detection

However, high-throughput genetic detection technology, such as high-throughput sequencing technology, solves the limitation of throughput, but its cost is high, the detection cycle is long, and the technical requirements for experimental operations and data analysis personnel are high, so it has not been popularized. applied clinically

[0005] In summary, the tests related to children's individualized safe drug use in the market do not completely cover children's common drugs, and cannot comprehensively evaluate the effectiveness and safety of children's common drugs in treatment.

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