Primer set, application, product and method for detecting SNP sites related to drug metabolism in children

A primer set and children's technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of long detection cycle, high cost, high technical requirement threshold, etc., and achieve low detection cost and accurate The effect of high reliability and shortened detection cycle

Active Publication Date: 2019-11-29
JIANGSU SIMCERE MEDICAL DEVICE CO LTD +2
View PDF10 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many detection methods with their own characteristics. PCR-based detection methods, such as ARMS-PCR, fluorescent quantitative PCR, digital PCR, etc., are easy to operate, fast, and simple in data analysis. However, due to the limitation of throughput, they cannot meet clinical requirements. The need for multiple genes and multiple locus detection
However, high-throughput genetic detection technology, such as high-throughput sequencing technology, solves the limitation of throughput, but its cost is high, the detection cycle is long, and the technical requirements for experimental operations and data analysis personnel are high, so it has not been popularized. applied clinically
[0005] In summary, the tests related to children's individualized safe drug use in the market do not completely cover children's common drugs, and cannot comprehensively evaluate the effectiveness and safety of children's common drugs in treatment.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer set, application, product and method for detecting SNP sites related to drug metabolism in children
  • Primer set, application, product and method for detecting SNP sites related to drug metabolism in children
  • Primer set, application, product and method for detecting SNP sites related to drug metabolism in children

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] The design and optimization of embodiment 1 primer, and the establishment of reaction system

[0100] Through the primer design software on the MassARRAY website, adjust the relevant parameters, complete the PCR and UEP primer design of 57 sites, export the designed primers and each parameter file, and synthesize the primers. Prepare amplification primer MIX and extension primer MIX according to the primer configuration table, and fine-tune the extension primer MIX until it meets the requirements. Primer testing and optimization were then performed. Specific steps are as follows:

[0101] (1) Dilute the genomic DNA sample to 10ng / μL, and prepare the PCR reaction MIX according to the table below (the following is the amount of a single sample)

[0102] Table 3 PCR reaction system

[0103]

[0104] Seal the film, mix with vortex for 30 seconds, centrifuge at 4000rpm for 1 minute, and put the plate on the PCR machine for the following thermal cycle:

[0105]

[0...

Embodiment 2

[0147] Verification of embodiment 2 reaction system

[0148] During the system optimization process of this example, 2 to 3 samples of each detection site were verified by Sanger sequencing, and the comparison results were all consistent, confirming that the detection results of this example are accurate. In this example, after confirming the optimal reaction system, a series of verification experiments were carried out, including accuracy, precision, personnel comparison and comparison experiments between different batches of primers. The specific verification scheme is as follows:

[0149] (1) Accuracy experimental verification plan: select one sample from each of the 57 sites for Sanger sequencing, and compare the results of Sanger sequencing and MassARRAY. If the consistency is greater than 95%, the verification is passed.

[0150] (2) Precision experiment verification plan: pick 3 peripheral blood samples and 3 corresponding oral swab samples, repeat 3 times for each sam...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a primer set, application, a product and a method for detecting SNP sites related to drug metabolism in children, and relates to the field of biotechnology. The primer set of the SNP sites related to drug metabolism in children provided by the invention can be used to detect the metabolism capacity of thermoanalgesics, metabolism capacity of respiratory system drugs, metabolism capacity of neurological and psychiatric drugs, metabolism capacity of cardiovascular and cerebrovascular drugs, metabolism capacity of anti-infective drugs, metabolism capacity of endocrine drugs, metabolism capacity of digestion system drugs, metabolism capacity of anesthetic drugs and metabolism capacity of chemotherapy and immunosuppressant. Specific detection of site mutation related to common drug metabolism in children can be realized by the primer set, the accuracy is high, the detection cycle can be tremendously shortened, and the detection cost is reduced at the same time. Moreover, the needs of a drug metabolism can be predicted by the detection results, the metabolism capacity of a variety of drugs can also be comprehensively evaluated, and scientific reference is providedfor the individualized medication of children.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer set, application, product and method for detecting SNP sites related to children's drug metabolism. Background technique [0002] Globally, about one-third of the deaths of patients each year are related to irrational drug use. In my country, about 2 million people are hospitalized due to adverse drug reactions every year, and about 200,000 people die due to adverse drug reactions. Children account for 1 / 3 of the deaths due to adverse drug reactions. Irrational drug use and adverse drug reactions caused by other reasons have become the fourth leading cause of death after cancer, cerebrovascular disease and heart disease. Children are in the growth and development stage, and the physiological functions of tissues and organs have not yet fully developed, so they have special reactions to drugs. Compared with adults, children's drug use has more potential safety hazards and n...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6883C12Q2600/106C12Q2600/156C12Q2531/113C12Q2533/101C12Q2565/627
Inventor 赵妍李翔陈珊珊李小方李杜衡肖念清
Owner JIANGSU SIMCERE MEDICAL DEVICE CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap