MTRR gene polymorphism detection primer system and reagent kit thereof

A gene polymorphism, detection kit technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. High sensitivity, wide application range, and the effect of a wide range of applications

Inactive Publication Date: 2015-09-09
上海赛安生物医药科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the methods for detecting MTRR gene polymorphism mainly include chip technology, probe primer technology, enzyme digestion, pyrosequencing technology and HRM analysis technology, etc., which are expensive, cumbersome or not accurate.

Method used

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  • MTRR gene polymorphism detection primer system and reagent kit thereof
  • MTRR gene polymorphism detection primer system and reagent kit thereof
  • MTRR gene polymorphism detection primer system and reagent kit thereof

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Embodiment 1

[0028] 1. The composition of the kit.

[0029] The MTRR gene polymorphism detection kit of this embodiment includes a reaction solution A for detecting that the c.66 site of the MTRR gene is A, and a reaction solution B for detecting that the c.66 site of the MTRR gene is G. , and the reaction solution C used to detect the β-actin gene as an internal control.

[0030] The preparation of reaction solution A, B, C is as follows:

[0031] 1. Preparation of reaction solution A (MIX A).

[0032] Reaction solution A is the reaction mixture for detecting the c.66 position of the MTRR gene, and the designed primers are aimed at the case where the c.66 position of the MTRR gene is A, including the positive for the case where the c.66 position of the MTRR gene is A. Arm primer (Primer F A66G For A), reverse universal primer corresponding to c.66 site of MTRR gene (Primer R A66G Rev Gen), SYBR Green mixed solution (2×SYBR GREEN Mix) and pure water (H 2 O).

[0033] See Table 1 for sp...

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Abstract

The invention relates to MTRR gene polymorphism detection primers and a detection system and reagent kit adopting the same. The primers comprise the forward primer used under the condition that an MTRR gene c.66 locus is A, the forward primer used under the condition that the MTRR gene c.66 locus is G, the universal reverse primer corresponding to the MTRR gene c.66 locus, the forward primer used for detecting a beta-actin gene, and the reverse primer used for detecting the beta-actin gene. The nucleotide sequence of the forward primer used under the condition that the MTRR gene c.66 locus is A is shown in SEQ ID No.1. The nucleotide sequence of the forward primer used under the condition that the MTRR gene c.66 locus is G is shown in SEQ ID No.2. The nucleotide sequence of the universal reverse primer corresponding to the MTRR gene c.66 locus is shown in SEQ ID No.3. The nucleotide sequence of the forward primer used for detecting the beta-actin gene is shown in SEQ ID No.4. The nucleotide sequence of the reverse primer used for detecting the beta-actin gene is shown in SEQ ID No.5. The reagent kit used for MTRR gene polymorphism detection is capable of quickly and conveniently carrying out detection, high in sensitivity and accuracy and low in cost.

Description

technical field [0001] The invention relates to a gene mutation detection product, a detection primer and a detection system used in the product, and belongs to the field of biotechnology. Background technique [0002] Mutations in the key enzyme genes in the folic acid metabolism pathway, such as the methionine synthase reductase (MTRR) gene and the methylenetetrahydrofolate reductase (MTHFR) gene, will lead to a decrease in folic acid utilization ability, DNA inheritance Decreased stability, resulting in abnormal embryonic development such as neural tube defects, cleft lip and palate, congenital heart disease, Down syndrome, etc.; and increased risk of hypertension during pregnancy and habitual abortion. [0003] Methionine Synthetic Reductase (MTRR) is a flavoprotein-related enzyme that can maintain the active state of methionine synthase and is essential for maintaining protein synthesis and one-carbon metabolism in the body - methionine The cycle plays an important reg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q1/6827C12Q2600/156
Inventor 赵新泰王明张长顺
Owner 上海赛安生物医药科技股份有限公司
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