Kit for rapid and simultaneous detection of MTHFR and MTRR gene polymorphisms and method thereof

Abstract

The invention discloses a kit for rapid and simultaneous detection of MTHFR and MTRR gene polymorphisms and a method thereof, and belongs to the technical field of gene detection. According to the kitfor rapid and simultaneous detection of MTHFR and MTRR gene polymorphisms, the gene polymorphisms include the following types of gene polymorphisms: MTHFR 677C being greater than T, MTHFR 1298A beinggreater than C and MTRR 66A being greater than G. The kit includes a sample treatment reagent used for rapid treatment of human whole blood samples, gene detection reagents which are premixed and single-packaged, a positive control and a negative control. The gene detection reagents comprise MTHFR and MTRR wild-type and mutant-type gene detection reagents. On the basis of guaranteeing the detection specificity and sensitivity, convenient, fast and efficient detection is realized, detection operating steps are reduced, detection reaction time is shortened, and the production cost and the detection cost are also reduced. The invention is beneificla to clinical detection, analysis and application.

Description

technical field [0001] The invention relates to a gene polymorphism detection kit and method, in particular to a kit and method for rapidly detecting MTHFR and MTRR gene polymorphisms at the same time, belonging to the technical field of gene detection. Background technique [0002] The MTHFR gene is located at lp36.3 on chromosome 1 and encodes 5,10-methylenetetrahydrofolate reductase. MTHFR is a key enzyme in the folate metabolism pathway, which reduces 5,10-methylenetetrahydrofolate to activity Strong 5-methyltetrahydrofolate, so that it can provide methylmethionine for homocysteine, folic acid and its metabolic intermediates play an important role in the physiological and biochemical reactions of the body, once the body produces folic acid Defects or folic acid metabolism enzyme defects will lead to abnormal cell cycle, abnormal DNA and protein methylation reactions, abnormal synthesis of DNA bases and other abnormal biochemical reactions, which will directly or indirect...

AI Technical Summary

Problems solved by technology

[0007] The main purpose of the present invention is to provide a kit and method for rapid detection of MTHFR and MTRR gene polymorphisms at the same time, to solve the problem of detecting MTHFR 677C>T, MTHFR 1298A>C, and MTRR 66A>G gene polymorphisms in the prior art. High sample processing requirements, long detection cycle, complicated operation, high cost, and low specificity, etc.

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