Primer, kit and method for detecting polymorphism of human MTRR gene by virtue of molecular beacon and application of primer

Abstract

The invention provides a primer for detecting the polymorphism of a human MTRR gene by virtue of a molecular beacon method. The primer comprises a primer pair and a probe, wherein the primer pair is used for amplifying polymorphic sites of rs1801394 (c.66 is more than G) and has the following nucleotide sequences, and the probe is used for detection. The invention further discloses a kit and method for detecting the polymorphism of the MTRR gene by virtue of the primer and the probe and application of the primer. By utilizing the primer, the polymorphism of the MTRR gene rs1801394 (c.66 is more than G) can be rapidly detected, and the result is accurate and easy to interpret. Compared with a sequencing method, the method has the advantages that a PCR product does not need to subjected to aseries of complex subsequent treatments, PCR amplification and detection are synchronously carried out, and the uncovering operation is not required in the whole detection process, so that the risk that the PCR product is polluted is reduced, the detection time is greatly shortened, and the detection cost is lowered.

Description

technical field [0001] The invention belongs to the technical field of gene detection, and in particular relates to a primer for detecting human MTRR gene polymorphism by a molecular beacon and an application thereof. Background technique [0002] Folic acid is a water-soluble B vitamin, an element necessary for the synthesis of nucleic acid, a substance necessary for cell growth and tissue repair, and an indispensable element in the process of embryonic development. Mutations in key genes in the folic acid metabolic pathway will reduce the activity of metabolic enzymes, resulting in folic acid metabolic disorders, resulting in folic acid deficiency. Folic acid deficiency in pregnant women can lead to serious consequences such as birth defects in newborns. The two most important genes involved in folate metabolism are the methylenetetrahydrofolate reductase gene (MTRR) and the methionine synthase reductase gene (MTRR). [0003] The full name of MTRR is 5-methyltetrahydrofo...

AI Technical Summary

Problems solved by technology

[0005] At present, the methods for polymorphic site analysis mainly include fluorescent quantitative PCR (qPCR), PCR-restriction fragment length polymorphism (PCR-Restriction Length Polymorphism, PCR-RFLP), amplification block mutation system-PCR (Amplification refractory mutation system PCR, ARMS-PCR), etc., but because these methods have many steps, are time-consuming and laborious, or require PCR post-processing, and are prone to pollution, they are not suitable for large-scale rapid detection of the population

Sequencing is the gold standard for nucleic acid sequencing. Although accurate, the steps are cumbersome and costly

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