One-step RT-qPCR (reverse transcription-quantitative polymerase chain reaction) human MTHFR (methylenetetrahydrofolate reductase) and MTRR (methionine synthase reductase) gene polymorphism expression detecting kit

A technology of MTHFR-C677T and detection kit, applied in the field of molecular biology, can solve problems such as easy generation of aerosol, cumbersome operation methods, laboratory pollution, etc., and achieve more specific results in detection results

Inactive Publication Date: 2017-10-10
厦门金诺威生物科技有限公司
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Problems solved by technology

This operation method is cumbersome and requires two-step reactions. At the same time, the cDNA needs to be diluted before the subsequen...

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  • One-step RT-qPCR (reverse transcription-quantitative polymerase chain reaction) human MTHFR (methylenetetrahydrofolate reductase) and MTRR (methionine synthase reductase) gene polymorphism expression detecting kit
  • One-step RT-qPCR (reverse transcription-quantitative polymerase chain reaction) human MTHFR (methylenetetrahydrofolate reductase) and MTRR (methionine synthase reductase) gene polymorphism expression detecting kit
  • One-step RT-qPCR (reverse transcription-quantitative polymerase chain reaction) human MTHFR (methylenetetrahydrofolate reductase) and MTRR (methionine synthase reductase) gene polymorphism expression detecting kit

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[0093] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention. If no specific technique or condition is indicated in the examples, it shall be carried out according to the technique or condition described in the literature in this field or according to the product specification. The reagents or instruments used were not indicated by the manufacturer, and they were all commercially available conventional products.

[0094] combined with Figure 7-21 , the best implementation mode of the present invention is as follows:

[0095] 1. Sample

[0096] 10 blood samples of women who are clinically ...

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Abstract

The invention discloses a one-step RT-qPCR human MTHFR and MTRR gene polymorphism expression detecting kit. The one-step RT-qPCR human MTHFR and MTRR gene polymorphism expression detecting kit comprises an MTHFR-C677T reaction buffer, an MTHFR-A1298C reaction buffer, an MTRR-A66G reaction buffer, a positive control, a negative control and a One step enzyme mixed liquor. The one-step RT-qPCR human MTHFR and MTRR gene polymorphism expression detecting kit is based on drug metabolism genetics as well as the advantages of high sensitivity and high specificity of fluorescent PCR probe technique and can directly detect translated products of expression areas for RNA (ribonucleic acid) sequences of MTHFR and MTRR, thereby, compared with existing DNA-real-time (deoxyribonucleic acid-real-time) fluorescent PCR methods, being closer to detection of whether expression proteins, namely, MTHFR and MTRR exist, and being higher in specificity of detecting results.

Description

technical field [0001] The invention relates to the field of molecular biology, and is an in vitro diagnostic reagent, specifically a detection kit for the polymorphism expression of human MTHFR and MTRR genes by one-step reverse transcription RT-qPCR. Background technique [0002] Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in the methionine-folate metabolic system, together with methionine synthase reductase (MTRR), it maintains the normal metabolism of folic acid. In addition, MTHFR is also involved in maintaining normal homocysteine ​​levels in the body. [0003] Mutations in the MTHFR and MTRR genes in vivo can reduce the activity of key enzymes encoding folic acid metabolism, causing folic acid metabolism disorders, resulting in reduced folic acid levels and hyperhomocysteinemia (HCY). [0004] For people with abnormal MTHFR and MTRR genes, the activity of MTHFR and MTRR enzymes is significantly reduced, resulting in folic acid metabolism disorders, re...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/156
Inventor 林家旺张秋芳崔勤何幸
Owner 厦门金诺威生物科技有限公司
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