Kit for genotyping folate metabolism gene

A folic acid metabolism gene, genotyping technology, applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve the problem of increasing the risk of neonatal birth defects or spontaneous abortion, adverse folic acid metabolism related gene detection, sequencing method Long detection cycle and other problems, to achieve the effect of increasing reliability and accuracy, saving reagent costs, and rapid detection

Active Publication Date: 2014-11-05
WUHAN CMLABS CO LTD
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Problems solved by technology

[0011] Genetic variants such as MTHFR and MTRR cause corresponding reductions in enzyme activity that inhibit the conversion of homocysteine ​​to methionine, leading to hypofolate and hyperhomocysteinemia, thereby increasing the risk of birth defects in newborns risk of spontaneous miscarriage
[0012] At present, the method of folic acid-related gene detection on the market is mainly sequencing method. Although sequencing method is the "gold

Method used

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  • Kit for genotyping folate metabolism gene
  • Kit for genotyping folate metabolism gene
  • Kit for genotyping folate metabolism gene

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Embodiment Construction

[0061] The present invention will be described in detail below in conjunction with specific embodiments and accompanying drawings, but the protection scope of the present invention is not limited to the following embodiments.

[0062] The kit for genotyping folic acid metabolism genes provided in this example includes a PCR reaction solution and a DNA hybridization membrane strip. The DNA hybridization membrane strip is composed of a matrix carrier and a probe, and four kinds of Probes for the MTHFR gene, two probes for the MTRR gene and two probes designed for the human genome, all of which are oligonucleotide sequences capable of hybridizing to the SNP sites of the above genes, the matrix The position of each probe on the carrier is as follows figure 1 shown. The matrix carrier can be glass slide, nylon membrane or other carrier on which probes can be fixed, in this embodiment it is nylon membrane.

[0063] Among the above probes, the nucleotide sequence of the probe T1 di...

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Abstract

The invention provides a kit for genotyping folate metabolism gene. The kit includes a PCR reaction liquid and a DNA hybrid membrane strip, the DNA hybrid film strip comprises a matrix vector and probes, four probes for MTHFR genes, two probes for MTRR genes and two probes designed for human genome are sequentially fixed on the matrix vector, each of the probes is an oligonucleotide sequence hybridized with the SNP locus of each of the corresponding gene, and the nucleotide sequences of the probes are represented by SEQ ID NO:1-8. The kit provided by the invention has the advantages of high sensitivity, rapid detection, good stability, realization of high or low flux detection, high flexibility, strong maneuverability for small clinical samples in some hospitals, less equipment investment and low cost.

Description

technical field [0001] The invention relates to a gene detection technology related to folic acid metabolism ability, which belongs to the field of molecular biology gene chip technology, specifically belongs to the SNP polymorphism detection method in the field of in vitro diagnostic reagents, and more specifically uses multiple polymerase chain reaction technology and Reverse dot hybridization technique. Background technique [0002] China is one of the countries with a high incidence of birth defects in the world, and the number of children with birth defects every year accounts for about 20% of the world. Common birth defects in my country include neural tube defects, congenital heart disease, cleft lip and palate, and hypospadias. According to statistics from the Chinese Center for Disease Control and Prevention, there are 800,000 to 1.2 million birth defects every year, and an average of one defect is born every 30 seconds. Among them, in addition to 20%-30% of child...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6827C12Q1/6858C12Q2565/625C12Q2563/131C12Q2531/113C12Q2537/143
Inventor 徐志勇秦伟
Owner WUHAN CMLABS CO LTD
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