The application discloses a monkeypox
virus whole
genome capturing method, a primer group and a kit. Four groups of specific primer pools are designed for the monkeypox
virus genome, and a high-fidelity
enzyme system with high sustained synthesis capability is matched to carry out targeted capture on the monkeypox
virus nucleic acid, so that the whole length of the monkeypox virus
genome can be effectively amplified, and the application prospect is wide.