Tissue culture seedling-growing method for bird's-net fern

A bird's nest fern and plant technology, applied in the field of group cultivation of bird's nest fern plants, can solve the problems of not being easy to obtain a large number of seedlings, high temperature and humidity requirements, long cycle of spore seeding, etc., and achieve great use and promotion value , reduce production costs and improve the survival rate of hardening seedlings

Inactive Publication Date: 2006-11-29
慈溪市蔬菜开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen from the above that the spore seeding method has a relatively long cycle, and more importantly, it has high requirements on temperature and humidity. Under the natural conditions in Zhejiang Province, the spore seeding method has a very low spore germination rate, and it is difficult to differentiate and emerge. A large number of seedlings are obtained, and it is difficult to be used in production
In addition, because the spores of bird's nest fern are small and fluffy, it is difficult to obtain materials and disinfect them, the production operability is poor, and they are prone to variation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Medium

[0031] Initial induction medium: improved MS+6-BA2.0mg / L+NAA0.1mg / L+AC1.0g / L. Among them, the modified MS contains NH 4 NO 3 1410.5mg / L, KNO 3 1900mg / L, MgSO 4 ·7H 2 O370mg / L, KH 2 PO 4 170mg / L, Ca(NO 3 ) 2 4H 2 O706.8mg / L, the amount of trace elements, iron salts, and organic components is the same as MS.

[0032] Proliferation medium: MS+6-BA0.5mg / L+AC0.5g / L.

[0033] Rooting medium: 1 / 2MS (macroelements halved)+NAA0.2mg / L+IBA0.1mg / L.

[0034] The pH of the above-mentioned medium before high-temperature and high-pressure sterilization (121°C-126°C, 103kPa~137kPa) is between 5.9-6.0, and the initial induction medium and proliferation medium are respectively added with common edible sucrose 30g / L and strength 900g / cm 2Agar powder 5.0g / L, common edible sucrose 20g / L and strength 900g / cm were added to the rooting medium 2 Agar powder 5.0g / L.

[0035] 2. Cultivate environmental conditions

[0036] Initial culture and proliferation culture The cul...

Embodiment 2

[0046] In this embodiment, except for the culture medium used, other methods are the same as in Embodiment 1.

[0047] Initial induction medium: improved MS+6-BA3.0mg / L+NAA0.2mg / L+AC1.0g / L. Among them, the modified MS contains NH 4 NO 3 1410.5mg / L, KNO 3 1900mg / L, MgSO 4 ·7H 2 O370mg / L, KH 2 PO 4 170mg / L, Ca(NO 3 ) 2 4H 2 O706.8mg / L, the amount of trace elements, iron salts, and organic components is the same as MS.

[0048] Proliferation medium: MS+6-BA0.5mg / L+AC0.5g / L.

[0049] Rooting medium: 1 / 2MS (macronutrients halved)+NAA0.1mg / L+IBA0.2mg / L.

Embodiment 3

[0051] In this embodiment, except for the culture medium used, other methods are the same as in Embodiment 1.

[0052] Initial induction medium: improved MS+6-BA2.5mg / L+NAA0.3mg / L+AC1.0g / L. Among them, the modified MS contains NH 4 NO 3 1410.5mg / L, KNO 3 1900mg / L, MgSO 4 ·7H 2 O370mg / L, KH 2 PO 4 170mg / L, Ca(NO 3 ) 2 4H 2 O706.8mg / L, the amount of trace elements, iron salts, and organic components is the same as MS.

[0053] Proliferation medium: MS+6-BA0.5mg / L+AC0.5g / L.

[0054] Rooting medium: 1 / 2MS (macroelements halved)+NAA0.2mg / L+IBA0.1mg / L.

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Abstract

A tissue culture method for new pteris fern includes such steps as obtain aseptic material, primary culture with the culture medium containing MS, 6-BA, NAA and AC, reproduction culture with the culture medium containing MS, 6-BA and AC, rooting culture with the culture medium containing 1/2 MS, NAA and IB, and intelligent culture of seedlings.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for group seedling cultivation of bird's nest ferns. Background technique [0002] Bird's Nest Fern (Asplenium nidus) Asplenium nidus is a perennial evergreen epiphytic fern with a plant height of up to 1 1.2 m to 1.2 m, short rhizomes, with fibrous branches and curls at the top. The leaves are clustered at the top of the short stem, arranged radially around, in the shape of a bird's nest, with cylindrical petioles, about 5 cm long, broad lanceolate leaves, herbaceous, light green, smooth on both sides, about 100 cm long. The bird's nest fern has a plump plant shape, green leaves, likes a cool environment, is relatively low temperature resistant, has extensive maintenance and management, and has strong resistance to diseases and insect pests. It has strong adaptability and high ornamental value. Good foliage material. Bird's nest fern is also the most wid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 杨广瑜
Owner 慈溪市蔬菜开发有限公司
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