Industrialized Tissue Culture and Rapid Propagation Technology of Tamarix

A technology of tamarisk and tissue culture seedlings, applied in gardening tools/equipment, plant regeneration, gardening, etc., can solve problems such as difficulty in large-scale popularization and application, inability to realize a variety of tamarisk factory seedlings, etc. High, high survival rate effect

Active Publication Date: 2020-11-20
SHANDONG NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Although some progress has been made in the tissue culture of Tamarix, most of them are still in the laboratory stage, and the existing research is only on the optimization of various conditions and the establishment of tissue culture system for one species of Tamarix, and it is impossible to achieve a variety of Tamarix. It is difficult to popularize and apply the unified factory seedling cultivation

Method used

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  • Industrialized Tissue Culture and Rapid Propagation Technology of Tamarix
  • Industrialized Tissue Culture and Rapid Propagation Technology of Tamarix
  • Industrialized Tissue Culture and Rapid Propagation Technology of Tamarix

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1 Tamarix explant disinfection system

[0052] Experimental materials: cuttings of three kinds of Tamarix chinensis Lour., Tamarix austromongolica Nakai, and Tamarix gallica Bunge were collected from Dongying in May 2016. The new shoots extracted from the cutting potted seedlings of Tamarix are explants.

[0053] For Chinese Tamarix, choose 75% C 2 h 5 OH(s) and 0.1% HgCl 2 (min), using two-factor three-level orthogonal L 9 (3 2 ) (as shown in Table 1), with the MS medium as the basic medium, first the tamarisk tender branches clipped are cleaned, washed clean, cleaned with cotton from bottom to top, rinsed with flowing tap water after cleaning, Then cut each section of Tamarix twigs into 3-4cm, rinse with flowing water for 30 minutes, and then take it to the ultra-clean workbench. In the ultra-clean workbench, first rinse it with sterile water three times, then soak it with 75% alcohol for a certain period of time, then soak it with 0.1% mercury liter ...

Embodiment 2

[0060] Example 2 Tissue Culture and Rapid Propagation System of Tamarix ganmeneng

[0061] 2.1 Experimental method

[0062] (1) Explant: the new tender shoots extracted from the cutting potted seedlings of Tamarix kambifolia are used as explants.

[0063] (2) Disinfection system: use 75% C 2 h 5 OH(s) and 0.1% HgCl 2 (min), the disinfection time is 30s and 9min respectively.

[0064] (3) Proliferation culture: Aseptic cultured sterile vaccines, MS medium is the basic medium, hormones are 6-BA and NAA, and 5×3 interactive experiments are set up (as shown in Table 3)

[0065] (4) Cultivation of strong seedlings: the clustered seedlings produced by the multiplication culture of three kinds of Tamarix willow were transferred to MS medium for strong seedling cultivation, and cultivated for 20 days.

[0066] (5) Rooting culture: the tissue cultured seedlings after the strong seedlings have grown high are cut into small sections of about 3 cm, and used as materials for rooting c...

Embodiment 3

[0081] Example 3 Tissue culture and rapid propagation system of Tamarix safflower and multibranches

[0082] 3.1 Experimental method

[0083] (1) Establishment of the disinfection system: the new shoots extracted from the potted seedlings are explants, and 75% C 2 h 5 OH(s) and 0.1% HgCl 2 (min), the disinfection time is 30s and 9min respectively.

[0084] (2) Proliferation culture: Aseptic cultured sterile vaccines, MS medium is the basic medium, hormones are 6-BA and NAA, and 5×3 interactive experiments are set up (as shown in Table 3)

[0085] (3) Cultivation of strong seedlings: the clustered seedlings produced by the multiplication culture of three kinds of Tamarix willow were transferred to MS medium for strong seedling cultivation, and cultivated for 20 days.

[0086] (4) Rooting culture: the tissue cultured seedlings after the strong seedlings have grown high are cut into small sections of about 3 cm, and used as materials for rooting culture. The selected basic m...

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Abstract

The invention discloses an industrial tissue culture and rapid propagation technology for tamarix chinensis. Unified tissue culture and rapid propagation breeding of various tamarix chinensis is realized finally through explant selection, establishment of a disinfection system, multiplication culture, combination of strong seedling culture and rooting culture as well as exercising seedling optimization, several key nodes including initiation rate, quantity of cluster buds on stems, rooting rate, root number, root length and survival rate of exercising seedlings for tissue culture breeding are optimized and controlled, the survival rate of the exercising seedlings with higher consistency is obtained finally, the effects of high initiation rate, many cluster buds, high survival rate and high reproduction coefficient are realized, great convenience and technical support are provided for industrial breeding of the tamarix chinensis, and the technology is quite suitable for industrial breeding production.

Description

technical field [0001] The invention belongs to the technical field of tree tissue culture, and in particular relates to an industrialized tissue culture rapid propagation technology of Tamarix, in particular to a large-scale seedling production technology applicable to various Tamarix species. Background technique [0002] The rapid economic development has seriously affected the natural environment. The saline soil in the world exceeds 800 million hectares (Munns and Tester, 2008), accounting for about 6.5% of the world's total area. The total area of ​​saline-alkali soil in my country is about 3.6×10 7 hm 2 , accounting for 4.88% of the country's usable land area, is one of the main limiting factors for my country's agriculture, urban greening and ecological construction. [0003] The plants of the genus Tamarix L. are shrubs or small trees of the order Caryophyllales (Tamaricaceae), and the plants of the genus Tamarix are widely distributed all over the world, mainly g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王宝山任义芳陈敏庞彩红
Owner SHANDONG NORMAL UNIV
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