Low temperature seedling hardening method for psammosilene tunicoides tissue culture seedling

A technology for tissue culture and hardening of seedlings, which is applied in the field of low-temperature seedling hardening of Tiesuo tissue culture seedlings, which can solve the problems of water content, high enzyme activity, low survival rate of hardened seedlings and transplanting, and low stress resistance of Tiesuo, and achieve yellowing Fewer cases, faster artificial seedling cultivation, good consistency

Inactive Publication Date: 2016-06-29
SOUTHWEST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In conventional seedling hardening, methods such as strong light hardening, natural light transitional hardening, and small shed hardening are often used. The disadvantages are: Jin Tiesuo has low soluble sugar content, high water content, and high enzyme activity in a high-temperature environment, which leads to Jin Tiesuo’s resistance. The resistance is low, so that the survival rate of Jintiesuo seedling transplanting is about 50%.
[0004] Jintiesuo is in a high-temperature environment, and the slow seedling period after transplanting is longer, and the probability of being infected by root rot and stem rot is higher, which makes the survival rate of Jintiesuo hardened seedlings transplanted lower and the pollution rate higher

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Before the hardening of Jintiesuo tissue culture seedlings, select the bottled seedlings with good growth condition, light green to dark green leaf color, and 4cm seedling height, and put them into frames for later use;

[0019] 2. Move the golden iron lock bottle seedlings to be transplanted into the seedling hardening room, and fumigate them with potassium permanganate and formaldehyde for 20 minutes, which has achieved the purpose of killing the fungus on the surface of the bottle;

[0020] 3. Cultivate the fumigated Tiesuo tissue culture seedlings in a well-ventilated environment with an ambient temperature of 17°C, a light intensity of 1900Lx, and a light time of 12h / d for 7 days. During the cultivation process, it can be seen that the Tiesuo tissue culture seedlings grow slowly, and some of them are too young Tender bottle seedlings have obvious stagnation at the height of the seedlings;

[0021] 4. After 7 days of transitional cultivation, the cuticle of the l...

Embodiment 2

[0026] 1. Before the hardening of Jintiesuo tissue culture seedlings, select the bottled seedlings with good growth condition, light green to dark green leaf color, and 3cm seedling height, and put them into frames for later use;

[0027] 2. Move the golden iron lock bottle seedlings to be transplanted into the seedling hardening room, and fumigate them with potassium permanganate and formaldehyde for 20 minutes, which has achieved the purpose of killing the fungus on the surface of the bottle;

[0028] 3. Cultivate the fumigated Tiesuo tissue culture seedlings in a well-ventilated environment with an ambient temperature of 16°C, light intensity of 2000Lx, and light time of 12h / d for 7 days. During the cultivation process, it can be seen that the Tiesuo tissue culture seedlings grow slowly, and some of them are too young Tender bottle seedlings have obvious stagnation at the height of the seedlings;

[0029] 4. After 7 days of transitional cultivation, the cuticle of the leaf ...

Embodiment 3

[0034] 1. Before the hardening of Jintiesuo tissue culture seedlings, select the bottled seedlings with good growth condition, light green to dark green leaf color, and 5cm seedling height, and put them into frames for later use;

[0035] 2. Move the golden iron lock bottle seedlings to be transplanted into the seedling hardening room, and fumigate them with potassium permanganate and formaldehyde for 20 minutes, which has achieved the purpose of killing the fungus on the surface of the bottle;

[0036] 3. Cultivate the fumigated Tiesuo tissue culture seedlings in a well-ventilated environment with an ambient temperature of 18°C, a light intensity of 1800Lx, and a light time of 12h / d for 7 days. During the cultivation process, it can be seen that the Tiesuo tissue culture seedlings grow slowly, and some of them are too young Tender bottle seedlings have obvious stagnation at the height of the seedlings;

[0037] 4. After 7 days of transitional cultivation, the cuticle of the l...

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Abstract

The invention relates to a low temperature seedling hardening method for a psammosilene tunicoides tissue culture seedling. The method comprises the steps: transferring psammosilene tunicoides bottled seedling into a seedling hardening culture chamber, and fumigating in a closed manner for 20 min by virtue of potassium permanganate and formaldehyde; culturing the tissue culture seedling for 7 days in an environment with good ventilation at the ambient temperature of 16 to 18 DEG C, the light intensity of 1800 to 2000 Lx and the lighting time of 12 h/d; adjusting the temperature to 11 to 13 DEG C, adjusting the light intensity to 1500 to 1800 Lx, continuously culturing for 14 days, then taking out the psammosilene tunicoides tissue culture seedling by virtue of tweezers, soaking in a 800-time carbendazim disinfectant for 2 h, air-drying, and then transplanting the psammosilene tunicoides tissue culture seedling into a seedling hardening matrix. On the basis that the content of soluble sugar of plants is increased in a low temperature environment, the water content is reduced, the enzyme activity is reduced and air holes are closed, the stress resistance of the plant is finally improved, the rejuvenation period is obviously shortened after the seedling is transplanted, and the probability of the plant infected by root rot and stem rot is reduced, so that the transplanting survival rate is increased to 95 percent or more, and the seedling hardening transplanting survival rate is greatly increased.

Description

technical field [0001] The invention relates to a low-temperature seedling hardening method for tissue-cultured seedlings of Jintiesuo, which belongs to the technical field of rapid propagation of traditional Chinese medicinal materials. Background technique [0002] Psammosilenetunicoides (Psammosilenetunicoides W.C.WuetC.Y.Wu) is a plant belonging to the genus Psammosilenetunicoides (Psammosilenetunicoides W.C.WuetC.Y.Wu), also known as Dudingzi, Kunming Arrhythmia, Centipede Seven, and Duyeqi, etc. It is mainly distributed in Yunnan, Sichuan, Guizhou and parts of Tibet. It is a unique single species of plant in China. It has a long history of folk medicine. It was first recorded in "Southern Yunnan Materia Medica", and then included in the 1977 edition of "Pharmacopia of the People's Republic of China". It is mainly used for bruises, rheumatic pain, wound bleeding, and swelling Pus drainage, etc., have the functions of dispelling blood stasis, drainage of pus, detumescenc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01H4/00
CPCA01H4/00A01G22/00
Inventor 陈杰王永周茂嫦徐庆祝赵健雄
Owner SOUTHWEST FORESTRY UNIVERSITY
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