A kind of Dendrobium officinale seedling production and seedling hardening synchronous method

Abstract

The invention belongs to the field of traditional Chinese medicinal materials, and relates to a method for synchronous production and hardening of Dendrobium candidum seedlings, in particular to a method for multiplying Dendrobium candidum tissue-cultured seedlings by using an intermittent submerged liquid culture system and hardening the seedlings in the same reactor . The invention uses aseptic dendrobium officinale seeds, protocorms and tissue culture seedlings as inoculation materials, obtains a large number of tissue culture plantlets of dendrobium officinale through inoculation and culture in a batch immersion culture system, and completes seedling hardening in the same reactor. The invention provides a method with high efficiency and low cost for the production of dendrobium officinale tissue culture seedlings.

Description

technical field [0001] The invention relates to a method for synchronously producing and hardening Dendrobium officinale seedlings, belonging to the field of seedling raising of traditional Chinese medicinal materials, and in particular to a method for multiplying tissue cultured seedlings of Dendrobium officinale by using an intermittent immersion liquid culture system and synchronously hardening the seedlings. technical background [0002] Dendrobium officinale ( Dedrobium candidum Wall1exLindl), also known as black knot grass, belongs to the epiphytic orchid family of Orchidaceae, is a traditional precious Chinese medicinal material, and is known as "the first of the nine Chinese fairy grasses" by the people. Listed as the top grade in "Shen Nong's Materia Medica", it has the functions of nourishing yin and clearing heat, promoting body fluid and benefiting stomach, relieving cough and moistening lung, moistening throat and improving eyesight, improving human immunity, pr...

AI Technical Summary

Problems solved by technology

However, the traditional method has many unavoidable disadvantages for the acquisition of Dendrobium candidum tissue culture seedlings: 1) Due to the sealed culture, gas exchange cannot be carried out during the cultivation process, resulting in vitrification of Dendrobium candidium tissue culture seedlings and poor seedling quality. Poor, low transplanting survival rate; 2) A large number of transfer operations are required during the cultivation process, and discarded medium needs to be disposed of, which increases labor costs; 3) Traditional plant tissue culture requires protocorm formation, In the process of cluster bud propagation, branch culture and root culture, after the tissue culture seedlings become seedlings, they need to be bottled, washed, sterilized and substrate cultivated. The survival rate of hardened seedlings is low, but the operation is cumbersome and requires specific facilities and conditions. , leading to higher production costs

The two above-mentioned Dendrobium officinale seedling hardening methods are the expansion of tissue culture seedlings and the hardening of seedlings separately, especially the seedlings obtained based on conventional solid medium culture rather than the Dendrobium officinale seedlings cultured in plant bioreactor liquid; Based on the method of obtaining seedlings and transplanting, the technical difficulty is high, the survival rate is difficult to grasp, the labor input is increased, and there is some damage to the tissue culture seedlings. At present, there is no relevant report on the simultaneous production of Dendrobium candidum tissue culture seedlings and hardening

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