A kind of Dendrobium officinale seedling production and seedling hardening synchronous method

A technology for Dendrobium officinale and seedling refining, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of increased labor input, tissue culture seedling damage, and high production costs, and achieves improved seedling survival rate, reduced Production cost, effect of saving production cost

Inactive Publication Date: 2016-01-06
NANJING BIOFUNCTION CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional method has many unavoidable disadvantages for the acquisition of Dendrobium candidum tissue culture seedlings: 1) Due to the sealed culture, gas exchange cannot be carried out during the cultivation process, resulting in vitrification of Dendrobium candidium tissue culture seedlings and poor seedling quality. Poor, low transplanting survival rate; 2) A large number of transfer operations are required during the cultivation process, and discarded medium needs to be disposed of, which increases labor costs; 3) Traditional plant tissue culture requires protocorm formation, In the process of cluster bud propagation, branch culture and root culture, after the tissue culture seedlings become seedlings, they need to be bottled, washed, sterilized and substrate cultivated. The survival rate of hardened seedlings is low, but the operation is cumbersome and requires specific facilities and conditions. , leading to higher production costs
The two above-mentioned Dendrobium officinale seedling hardening methods are the expansion of tissue culture seedlings and the hardening of seedlings separately, especially the seedlings obtained based on conventional solid medium culture rather than the Dendrobium officinale seedlings cultured in plant bioreactor liquid; Based on the method of obtaining seedlings and transplanting, the technical difficulty is high, the survival rate is difficult to grasp, the labor input is increased, and there is some damage to the tissue culture seedlings. At present, there is no relevant report on the simultaneous production of Dendrobium candidum tissue culture seedlings and hardening

Method used

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  • A kind of Dendrobium officinale seedling production and seedling hardening synchronous method
  • A kind of Dendrobium officinale seedling production and seedling hardening synchronous method

Examples

Experimental program
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Effect test

Embodiment 1

[0046] (1) Selection of explants: Cut the sterilized Dendrobium officinale fruit into a small mouth, and let the seeds scatter into the sterilized intermittent immersion culture device.

[0047] (2) Seedling cultivation: The parameters of the intermittent immersion device are: inoculation density: 0.5 fruit clips / L; immersion frequency: 10min / 6h; cultivation time: 75d; the formula of the culture medium for cultivation is 1 / 2Ms, 0.2mg / L NAA, 30g / L sucrose, 20g / L banana juice or potato juice, pH 6.2; culture conditions: light intensity 1500~1800lux, light time 12h / d, temperature 25±1℃.

[0048] (3) Seedling hardening: In the same reactor, replace the culture medium of the above-mentioned liquid culture with the culture medium of gradually decreasing sucrose concentration without changing the immersion frequency of the reactor. Under the condition of ±3°C, cultivate for 15 days to complete the gradual adaptation stage of the tissue culture seedlings. After the gradual adaptation...

Embodiment 2

[0051] (1) Selection of explants: Cut the sterilized Dendrobium officinale fruit into a small mouth, and let the seeds scatter into the sterilized intermittent immersion culture device.

[0052] (2) Liquid culture: The parameters of the intermittent immersion device are: inoculation density: 0.5 fruit clips / L; immersion frequency: 5min / 6h; culture time: 90d; 30g / L sucrose, 20g / L banana juice or potato juice, pH 6.2; culture conditions: light intensity 1500~1800lux, light time 12h / d, temperature 25±1℃.

[0053] (3) Seedling hardening: In the same reactor, replace the above-mentioned liquid culture culture solution with a culture solution with a gradually reduced sucrose concentration, without changing the immersion frequency of the reactor, when the light intensity is 1800~2000lux, the light time is 14h / d, and the temperature is 24 ±3°C, cultured for 25 days to complete the gradual adaptation stage of tissue culture seedlings. After the gradual adaptation stage of the tissue-c...

Embodiment 3

[0056] (1) Explant selection: Inoculate the protocorms without deformity and vitrification of the first generation after sowing sterile seeds into the batch immersion culture device.

[0057] (2) Liquid culture: The parameters of the intermittent immersion device are: inoculation density: 2.5g / L; immersion frequency: 5min / 6h; culture time: 80d; L sucrose, 20g / L banana juice or potato juice, pH6.2; culture conditions: light intensity 1500~1800lux, light time 12h / d, temperature 25±1℃.

[0058] (3) Seedling hardening: In the same reactor, replace the above-mentioned liquid culture culture solution with a culture solution with a gradually reduced sucrose concentration, without changing the immersion frequency of the reactor, when the light intensity is 1800~2000lux, the light time is 11h / d, and the temperature is 24 ±3°C, cultivate for 17 days, and complete the gradual adaptation stage of tissue culture seedlings. After the gradual adaptation stage of the tissue-cultured seedling...

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Abstract

The invention belongs to the field of traditional Chinese medicinal materials, and relates to a method for synchronous production and hardening of Dendrobium candidum seedlings, in particular to a method for multiplying Dendrobium candidum tissue-cultured seedlings by using an intermittent submerged liquid culture system and hardening the seedlings in the same reactor . The invention uses aseptic dendrobium officinale seeds, protocorms and tissue culture seedlings as inoculation materials, obtains a large number of tissue culture plantlets of dendrobium officinale through inoculation and culture in a batch immersion culture system, and completes seedling hardening in the same reactor. The invention provides a method with high efficiency and low cost for the production of dendrobium officinale tissue culture seedlings.

Description

technical field [0001] The invention relates to a method for synchronously producing and hardening Dendrobium officinale seedlings, belonging to the field of seedling raising of traditional Chinese medicinal materials, and in particular to a method for multiplying tissue cultured seedlings of Dendrobium officinale by using an intermittent immersion liquid culture system and synchronously hardening the seedlings. technical background [0002] Dendrobium officinale ( Dedrobium candidum Wall1exLindl), also known as black knot grass, belongs to the epiphytic orchid family of Orchidaceae, is a traditional precious Chinese medicinal material, and is known as "the first of the nine Chinese fairy grasses" by the people. Listed as the top grade in "Shen Nong's Materia Medica", it has the functions of nourishing yin and clearing heat, promoting body fluid and benefiting stomach, relieving cough and moistening lung, moistening throat and improving eyesight, improving human immunity, pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈集双张本厚贾明良金磊磊覃佳佳
Owner NANJING BIOFUNCTION CO LTD
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