A kind of cultivation method of capsicum disease-resistant homozygote

A breeding method and homozygous technology, applied in the field of crop breeding, can solve the problems of long breeding cycle and low breeding success rate, and achieve the effects of convenient production, promotion of callus formation, and production cost saving

Active Publication Date: 2021-06-01
SHANDONG SHOUGUANG VEGETABLE SEED IND GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The breeding cycle is long and the breeding success rate is low. Therefore, it is urgent to establish a complete set of efficient breeding system to improve breeding efficiency and effectively serve production.

Method used

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  • A kind of cultivation method of capsicum disease-resistant homozygote
  • A kind of cultivation method of capsicum disease-resistant homozygote

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Take capsicum anther as explant, establish 6 kinds of different mediums to handle, each repeat 3 times, specific technical scheme is as follows:

[0051] Treatment 1: Carry out capsicum anther haploid culture according to the method of the present invention.

[0052] On sunny days, flower buds with a length of 4.0-4.49 mm were collected from pepper plants in the greenhouse of the Shouguang Vegetable Group Breeding Base, pretreated at 4°C for 4 days, added 3-4 drops of Tween 80, and rinsed in running water for 1.5 hours; % alcohol disinfection for 15 seconds, rinsed with sterile water 4 times; disinfected with 0.1% mercuric chloride (mercuric chloride) for 10 minutes, rinsed with sterile water 4 times. The anthers were inoculated on MS basic medium plus supplementary components, and the supplementary components were 0.05 mg·L -1 6-BA, 0.01mg·L -1 2,4-D, 30g·L -1 Trehalose, 20g·L -1 Black fruit wolfberry juice filtrate, 7g·L -1 Agar powder, pH5.8. The inoculation ...

Embodiment 2

[0078] Practical application: Carry out capsicum anther haploid culture according to the method of the present invention.

[0079] Collect flower buds with a length of 4.0-4.49 mm from pepper plants in the greenhouse of Shouguang Vegetable Group Breeding Base on a sunny day, pretreat them at 4°C for 4 days, add 4 drops of Tween 80, and rinse them in running water for 1.5 hours; then use 75% alcohol Disinfect for 15 seconds, rinse with sterile water 4 times; disinfect with 0.1% mercuric chloride (mercuric chloride) for 10 minutes, rinse with sterile water 4 times. The anthers were inoculated on MS basic medium, and the medium formula was 0.05 mg·L -1 6-BA, 0.01mg·L -1 2,4-D, 30g·L -1 Trehalose, 20g·L -1 Black fruit wolfberry juice filtrate, 7g·L -1 Agar powder, pH 5.8. The inoculation density was 8 flower buds per 100 mm Petri dish. Then continue culturing in a tissue culture room with a temperature of 28°C during the day and a temperature of 20°C at night.

[0080] Wh...

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Abstract

The invention discloses a method for cultivating chili disease-resistant homozygotes. The method includes a tissue culture identification step and an anti-viral disease screening step. The tissue culture identification is based on conventional culture medium, using trehalose instead of sucrose and adding black wolfberry juice The nutrient composition of the filtrate was formulated to prepare a specific medium formula for the selection of explants, explant treatment, anther culture, acquisition of complete plants, colchicine treatment and ploidy identification of peppers to obtain homozygous seedlings; and use molecular The marker technology is used to screen anti-viral disease molecular markers to obtain anti-viral disease homozygous plants. The method of the present invention can significantly shorten the screening period, and quickly obtain homozygous anti-viral diseases of pepper; and the method is simple in operation, low in cost, high in survival rate after transplanting flower seedlings, strong in stress resistance, easy to popularize, and can be directly applied to production , have good economic and social benefits.

Description

technical field [0001] The invention relates to the technical field of crop breeding, in particular to a method for cultivating chili disease-resistant homozygotes. Background technique [0002] Pepper (Capsicum annuum L) is an important vegetable crop that originated in Central and South America and Mexico, and its annual planting area in China has reached more than 1 million hectares. In the 1960s, there were related reports on the virus source species of hot pepper (sweet) pepper virus disease. Since the 1970s, the harm of hot (sweet) pepper virus disease has become more and more serious, with a high incidence rate and fast spread. Generally, the output is reduced by about 30%, and in severe cases, it is as high as more than 60%, and even production is cut off. It has become the main production of hot (sweet) pepper. Limiting factor. Therefore, cultivating varieties resistant to viral diseases is the most effective prevention and control approach with significant econom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12Q1/686
CPCA01H4/001A01H4/008C12Q1/686
Inventor 武玉芬程琳潘子龙国家进韩宇睿王祥程斐王晓武李晓杰
Owner SHANDONG SHOUGUANG VEGETABLE SEED IND GRP CO LTD
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