Cultivation method of chili disease-resistant homozygote

A cultivation method and homozygous technology, applied in the field of crop breeding, can solve the problems of low breeding success rate and long breeding cycle, and achieve the effects of improving induction rate, success rate and stress resistance.

Active Publication Date: 2018-08-17
SHANDONG SHOUGUANG VEGETABLE SEED IND GRP CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The breeding cycle is long and the breeding success rate is low. Therefore, it is urgent to establish a c

Method used

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  • Cultivation method of chili disease-resistant homozygote
  • Cultivation method of chili disease-resistant homozygote

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Experimental program
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Effect test

Embodiment 1

[0050] Take capsicum anther as explant, establish 6 kinds of different mediums to handle, each repeat 3 times, specific technical scheme is as follows:

[0051] Treatment 1: Carry out capsicum anther haploid culture according to the method of the present invention.

[0052] On sunny days, flower buds with a length of 4.0-4.49 mm were collected from pepper plants in the greenhouse of the Shouguang Vegetable Group Breeding Base, pretreated at 4°C for 4 days, added 3-4 drops of Tween 80, and rinsed in running water for 1.5 hours; % alcohol disinfection for 15 seconds, rinsed with sterile water 4 times; disinfected with 0.1% mercuric chloride (mercuric chloride) for 10 minutes, rinsed with sterile water 4 times. The anthers were inoculated on MS basic medium plus supplementary components, and the supplementary components were 0.05 mg·L -1 6-BA, 0.01mg·L -1 2,4-D, 30g·L -1 Trehalose, 20g·L -1 Black fruit wolfberry juice filtrate, 7g·L -1 Agar powder, pH5.8. The inoculation ...

Embodiment 2

[0078] Practical application: Carry out capsicum anther haploid culture according to the method of the present invention.

[0079] Collect flower buds with a length of 4.0-4.49 mm from pepper plants in the greenhouse of Shouguang Vegetable Group Breeding Base on a sunny day, pretreat them at 4°C for 4 days, add 4 drops of Tween 80, and rinse them in running water for 1.5 hours; then use 75% alcohol Disinfect for 15 seconds, rinse with sterile water 4 times; disinfect with 0.1% mercuric chloride (mercuric chloride) for 10 minutes, rinse with sterile water 4 times. The anthers were inoculated on MS basic medium, and the medium formula was 0.05 mg·L -1 6-BA, 0.01mg·L -1 2,4-D, 30g·L -1 Trehalose, 20g·L -1 Black fruit wolfberry juice filtrate, 7g·L -1 Agar powder, pH 5.8. The inoculation density was 8 flower buds per 100 mm Petri dish. Then continue culturing in a tissue culture room with a temperature of 28°C during the day and a temperature of 20°C at night.

[0080] Wh...

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Abstract

The invention discloses a cultivation method of chili disease-resistant homozygote. The method includes steps: culturing and identifying tissue: utilizing a conventional culture medium, using trehalose to replace sucrose, adding lycium ruthenicum juice filtrate nutritional ingredients to prepare a specific culture medium formula, and performing explant selection, explant treatment, anther culture,complete plant acquisition, colchicine treatment and ploidy identification on chili to obtain homozygote seedlings; utilizing molecular marker technology for virus disease resistant molecular markerscreening to obtain virus disease resistant homozygote plants. The method can remarkably shorten screening period and quickly acquire the chili disease-resistant homozygote, is simple to operate, lowin cost and easy for popularization, can be directly applied in production and has good economic benefit and social benefit,, and anther cultured seedlings are high in survival rate and stress resistance after being transplanted.

Description

technical field [0001] The invention relates to the technical field of crop breeding, in particular to a method for cultivating chili disease-resistant homozygotes. Background technique [0002] Pepper (Capsicum annuum L) is an important vegetable crop that originated in Central and South America and Mexico, and its annual planting area in China has reached more than 1 million hectares. In the 1960s, there were related reports on the virus source species of hot pepper (sweet) pepper virus disease. Since the 1970s, the harm of hot (sweet) pepper virus disease has become more and more serious, with a high incidence rate and fast spread. Generally, the output is reduced by about 30%, and in severe cases, it is as high as more than 60%, and even production is cut off. It has become the main production of hot (sweet) pepper. Limiting factor. Therefore, cultivating varieties resistant to viral diseases is the most effective prevention and control approach with significant econom...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12Q1/686
CPCA01H4/001A01H4/008C12Q1/686
Inventor 武玉芬程琳潘子龙国家进韩宇睿王祥程斐王晓武李晓杰
Owner SHANDONG SHOUGUANG VEGETABLE SEED IND GRP CO LTD
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