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Open plant tissue culture and factory rapid propagation method

A tissue culture and open technology, applied in the fields of botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of low transplant survival rate, high cultivation cost, cumbersome operation procedures, etc., and achieve high seedling survival rate. , a wide range of choices, the effect of robust growth

Inactive Publication Date: 2011-10-19
崔刚
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Problems solved by technology

[0003] The object of the present invention is to solve the problems such as strict requirements on the environment of the tissue culture room in the current plant tissue culture seedling raising process, which need to be carried out under strict aseptic conditions, cumbersome operating procedures, low efficiency, high culture cost, and low transplanting survival rate. Systematic research has been carried out on tissue culture seedling technology, and a new method of plant open tissue culture factory seedling cultivation has been invented, which makes plant tissue culture out of the strict aseptic operating environment and operates in a natural and open environment with bacteria

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  • Open plant tissue culture and factory rapid propagation method

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Embodiment Construction

[0010] 1. Selection of culture container

[0011] In the present invention, however, an antibacterial medium is used instead of autoclaving. Therefore, the selection range of the culture container is large, and can be extended from the original necessary high-temperature sterilization container to various transparent containers that are not resistant to high-temperature sterilization.

[0012] 2. Preparation of antibacterial medium

[0013] After the culture medium is cooked, add different concentrations of Shannong No. 1 antibacterial agent according to different culture purposes. Use the PLT-G50 medium quantitative filling machine to fill the medium into the culture container, and the medium is prepared, and no high-pressure sterilization is required.

[0014] 3. Sterilization of inoculation equipment

[0015] However, in the present invention, the inoculation device is soaked in 75% alcohol, and the inoculation process does not need to be sterilized by burning, so that t...

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Abstract

An open plant tissue culture and factory rapid propagation method disclosed in the invention, is characterized by: using a transparent container, dispensing the boiled medium containing antibacterial agent Shannon 1 with different concentrations into a culture vessel to prepare a medium, sterilizing the inoculation room with alcohol for dust control and ultraviolet irradiation, cleaning the surface of the inoculation bench and hands with alcohol, opening a bottle cap, inoculating the plant to the medium by using an inoculator, and then sealing. The invention has the beneficial effects that: the culture container has a broad range of choice, a plastic cup, plastic casing with autoclaving resistance can be used as the culture container; the medium has no loss of components without autoclaving, hormone types have a broad range of choice; the inoculation can be directly carried out in an open and bacterial environment without a clean bench, the inoculation speed is 3-5 times traditional inoculation; by culturing in the open and bacterial greenhouse, developing from a heterotrophic mode of traditional tissue culture to a mode containing autotrophism and heterotrophism, tissue culture plants grow health and strong, the survival rate of the hardening seedling in the mode containing autotrophism and heterotrophism is significantly higher than that of traditional mode.

Description

technical field [0001] The invention relates to a method for plant open tissue culture and factory rapid propagation. Background technique [0002] The current methods of plant open tissue culture and industrialized rapid propagation generally use high-temperature-resistant glass bottles or plastic bottles, which need to be sterilized by high pressure. , blades, etc. are all sterilized by high pressure or alcohol lamp burning. The inoculation requirements are strict, the efficiency is low, and the cultivation environment is strict. Contents of the invention [0003] The object of the present invention is to solve the problems such as strict requirements on the environment of the tissue culture room in the current plant tissue culture seedling raising process, which need to be carried out under strict aseptic conditions, cumbersome operating procedures, low efficiency, high culture cost, and low transplanting survival rate. Systematic research has been carried out on tissu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 崔刚
Owner 崔刚
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