Subculture method for tissue culture seedlings of cercis gigantea lines

A technology of subculture and Bauhinia group, which is applied in the field of subculture of giant Bauhinia seedlings, can solve the problems of unsuitable industrial production of tissue cultured seedlings and high cost of zeatin, and achieve high rooting rate and survival rate of hardened seedlings. high effect

Active Publication Date: 2017-05-17
上海菁艺生物科技有限公司
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Problems solved by technology

This formula uses MS as the basic medium, and the value-added coefficient is 3.56. The zeatin (ZT) used in this experiment is expensive and not suitable for industrial production of tissue culture seedlings.

Method used

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  • Subculture method for tissue culture seedlings of cercis gigantea lines
  • Subculture method for tissue culture seedlings of cercis gigantea lines
  • Subculture method for tissue culture seedlings of cercis gigantea lines

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Experimental program
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Embodiment 1

[0044] (1) Experimental materials

[0045] Female parent material: Cut 1-2 year-old healthy branches with full buds from 4-year-old trees that are robust and free from diseases and insect pests, and plant them in indoor hydroponics. After the new shoots germinate, they will be used as the source of explant culture;

[0046] Subculture and rooting culture materials: The subculture period is 25-30 days, and the growth is vigorous, the leaves are stretched, and the plant height is 3-5cm. The giant bauhinia tissue culture seedlings are used as experimental materials for subculture and rooting culture;

[0047] (2) Specific experimental steps

[0048] A method for subculture of giant bauhinia tissue culture seedlings, the steps are as follows:

[0049] 1. Acquisition of sterile explants

[0050] Select 1-2 year old robust branches with bud packs for hydroponics indoors, cut into 1-2cm long terminal buds or stems with axillary buds after the new shoots germinate, cut off the leaves ...

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Abstract

The invention discloses a subculture method for tissue culture seedlings of cercis giganter lines. The subculture method is characterized by comprising the following steps: 1, obtaining sterile explants; 2, carrying out subculture multiplication on the cercis giganter lines; 3, carrying out rooting culture on the cercis giganter lines; 4, controlling subculture and rooting culture conditions of the cercis giganter lines; and 5, hardening and transplanting the tissue culture seedlings of the cercis giganter lines. The multiplication method for the tissue culture seedlings of the cercis giganter lines disclosed by the invention has the following advantages that (1) the multiplication coefficient is high and the average multiplication coefficient reaches above 5; (2) the rooting rate is high and the maximum rooting rate can reach 98%; and (3) the survival rate of hardened seedlings is high and averagely above 90%.

Description

technical field [0001] The invention relates to a method for cultivating tissue-cultured seedlings, in particular to a method for subculture of the tissue-cultured seedlings of the giant bauhinia. Background technique [0002] Giant Bauhinia (Cercis giganter Lines) is a deciduous tree of the Fabaceae / Hemataceae Bauhinia genus. The natural growth of large trees has a diameter at breast height of 60cm and a height of more than 15m. ". The giant bauhinia is a unique native tree species in my country, concentrated in subtropical regions. From Zunyi in Guizhou in the south to Luoning in Henan in the north; Lin'an in Zhejiang in the east and Shennongjia in Hubei in the west. The young leaves of Giant Bauhinia are purple-red, the flowers are pseudo-butterfly-shaped, lavender, bloom before the leaves from March to April, and the flowers are gorgeous. Street trees, shade trees and ornamental trees for greening. [0003] Giant Bauhinia is a tree species of Fabaceae Bauhinia. The r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G9/10
CPCA01G24/00A01H4/001A01H4/008
Inventor 冒文娟董举文李文剑张玉杰林大为张承妹苏涌
Owner 上海菁艺生物科技有限公司
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