Method for establishing general maize regeneration system

A corn and system technology, applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of difficult direct utilization, poor comprehensive economic properties, etc., and achieve the effect of improving the effect, increasing the survival rate, and reducing the cost

Active Publication Date: 2012-01-25
新疆康地种业科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, although maize regeneration systems are relatively mature, almost all reported maize regeneration systems are limited to a limited number of maize inbred lines, and their comprehensive economic traits are poor, so it is difficult to directly use them in breeding.

Method used

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  • Method for establishing general maize regeneration system
  • Method for establishing general maize regeneration system
  • Method for establishing general maize regeneration system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Establishment of general corn regeneration system

[0031] (1) Surface disinfection: select Condy 3 and Condy 4 of excellent corn inbred line seeds. The young ears can be used for 8-10 days after pollination. At this time, the length of the immature embryo of the immature seeds is 1.0-2.0mm. The outer bracts of the ear are removed, and the young ears are surface-disinfected with 70% alcohol, and rinsed with sterile water for 1-2 times .

[0032] (2) Induction of corn callus and embryogenic callus: Use dissecting tweezers to carefully pick out immature embryos on an ultra-clean workbench, connect the sterilized immature embryos to the induction medium, and incubate at 28°C for 2-3 Week; Induction medium formula adopts: N6 macroelement, B5 trace element, B5 vitamin, 0.04mg / L riboflavin, 0.04mg / L folic acid, 0.1mg / L niacin, 0.1mg / L D-calcium pantothenate, 0.04 mg / L p-toluic acid, sucrose 50g / L, 2.4-D is 0.4mg / L, 6-BA is 5mg / L. Condy 3 and Condy 4 induced white cal...

Embodiment 2

[0036] Example 2: Zheng 958, Establishment of Xianyu 335 corn regeneration system

[0037] (1) Surface disinfection: select Zheng 958 and Xianyu 335, which are excellent corn inbred line seeds. The young ears can be used for 9-11 days after pollination. At this time, the length of the immature embryo of the immature seeds is 1.0-2.0mm. The outer bracts of the ear are removed, and the young ears are surface-disinfected with 70% alcohol and rinsed with sterile water for 1-2 times .

[0038] (2) Induction of corn callus and embryogenic callus: Use dissecting tweezers to carefully pick out immature embryos on an ultra-clean workbench, connect the sterilized immature embryos to the induction medium, and incubate at 28°C for 2-3 Week; Induction medium formula adopts: N6 macroelement, B5 trace element, B5 vitamin, 0.04mg / L riboflavin, 0.04mg / L folic acid, 0.1mg / L niacin, 0.1mg / L D-calcium pantothenate, 0.04 mg / L p-toluic acid, sucrose 50g / L, 2.4-D is 0.4mg / L, 6-BA is 5mg / L. Zheng 958 a...

Embodiment 3

[0042] Example 3: Establishment of A188 and Syn 31 corn regeneration system

[0043] (1) Surface disinfection: Select the seeds of excellent maize inbred lines A188 and Zong 31. The young ears can be used for 8-11 days after pollination. At this time, the length of the immature embryo of the immature seeds is 1.0-2.0mm. The outer bracts of the ear are removed, and the young ears are surface-disinfected with 70% alcohol and rinsed with sterile water for 1-2 times .

[0044] (2) Induction of corn callus and embryogenic callus: Use dissecting tweezers to carefully pick out immature embryos on an ultra-clean workbench, connect the sterilized immature embryos to the induction medium, and incubate at 28°C for 2-3 Week; Induction medium formula adopts: N6 macroelement, B5 trace element, B5 vitamin, 0.04mg / L riboflavin, 0.04mg / L folic acid, 0.1mg / L niacin, 0.1mg / L D-calcium pantothenate, 0.04 mg / L p-toluic acid, sucrose 50g / L, 2.4-D is 0.4mg / L, 6-BA is 5mg / L. White callus was induced in...

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Abstract

The invention discloses a method for establishing a general maize regeneration system. The method comprises the following steps of: inoculating different maize inbred line embryos onto induction mediums with different hormone combinations, transferring an appropriate induced embryoid onto a differential medium to carry out high frequency differentiation on regenerated seedlings, transferring the regenerated seedlings onto a rooting medium to carrying out rooting induction so as to obtain a regenerated plant, wherein the formula of the induction medium comprises N6 marcoelement, B5 microelement, B5 vitamin, riboflavin (0.04mg / L), folic acid (0.04mg / L), nicotinic acid (0.1mg / L), D-calcium pantothenate (0.1mg / L), methyl benzoic acid (0.04mg / L), cane sugar (50g / L), 2.4-D (0.4mg / L) and 6-BA (5mg / L). The method disclosed by the invention has the characteristics of good generality to different maize inbred lines and shorter time for obtaining the regenerated plant and has important application value.

Description

Invention field [0001] The present invention relates to the field of biotechnology. Specifically, the present invention specifically relates to the technical field of establishing a universal corn regeneration system. Background technique [0002] Corn is the third largest food crop in the world, and its planting area is second only to rice and wheat. At the same time, corn is an important fodder crop. Therefore, increasing corn production and improving corn quality are related to China's future food security supply. Genetic improvement of maize through conventional breeding is now one of the main ways to increase maize production, but factors such as limited genetic resources and relatively long breeding cycles often limit its application potential. With the rapid development of biotechnology, breeders from various countries have widely used biotechnology to introduce some foreign genes that control excellent traits into corn to achieve the goal of cultivating new varieties of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王沛政林霞夏春兰王志安李新鹏
Owner 新疆康地种业科技股份有限公司
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