Seedling hardening method for iris tissue-cultured seedlings

A technology of iris group and tissue culture seedlings, applied in the agricultural field, can solve the problems of low survival rate of hardening seedlings, poor tissue culture effect, high cost, etc., and achieve the effects of shortening culture time, improving bactericidal effect, and strengthening control

Inactive Publication Date: 2020-06-30
INNER MONGOLIA HESHENG ECOLOGICAL SILVICULTURE CO LTD +1
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the defects of low survival rate of hardening seedlings in the process of tissue culture of Iris germanica in the prior art, resulting in poor tissue culture effect and high cost, thereby providing a kind of Iris tissue culture seedling hardening method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The present embodiment provides a kind of seedling hardening method of german iris tissue culture seedling, comprises the steps:

[0045] Cleaning and sterilization: Gently wash the roots of German iris tissue culture seedlings with a height of 6cm in warm water at 32°C to remove the medium attached to the roots to avoid damage to the roots and seedlings; then put the roots in a concentration of 0.5mg / L Soak in the bacteriocin solution for 3 minutes;

[0046] Transplanting: On October 2, 2018, the cleaned and sterilized rooted Iris germanica plantlets were transplanted into a pure vermiculite substrate with a humidity of 50%. Stretch and completely bury in the matrix, and compact the matrix around the tissue culture seedlings, and select tissue culture seedlings with similar growth heights to transplant into the same hole tray; after transplanting each hole tray, place it on the seedbed, A total of 14040 plants were transplanted; at this time, the german iris seedlings...

Embodiment 2

[0051] The present embodiment provides a kind of seedling hardening method of german iris tissue culture seedling, comprises the steps:

[0052] Cleaning and sterilization: Gently wash the roots of German iris tissue culture seedlings with a height of 9cm in warm water at 35°C to remove the medium attached to the roots to avoid damage to the roots and seedlings; then put the roots in a concentration of 0.60mg / L Soak in bacteria clear solution for 2.0min;

[0053] Transplanting: On December 15, 2018, the cleaned and sterilized rooted German iris tissue-cultured seedlings were transplanted into a pure vermiculite substrate with a humidity of 60%, and when transplanting, the roots of the tissue-cultured seedlings should be stretched and Completely bury in the matrix, and compact the matrix around the tissue culture seedlings, and select tissue culture seedlings with similar growth heights to transplant into the same hole tray; after transplanting each hole tray, place them on the...

Embodiment 3

[0058] The present embodiment provides a kind of seedling hardening method of german iris tissue culture seedling, comprises the steps:

[0059] Cleaning and sterilization: Gently wash the roots of German iris tissue culture seedlings with a height of 8cm in warm water at 30°C to remove the medium attached to the roots to avoid damage to the roots and seedlings; then put the roots in a concentration of 0.40mg / L Soak in the bacteria clear solution for 4 minutes;

[0060] Transplanting: On February 20, 2019, the cleaned and sterilized rooted German iris tissue-cultured seedlings were transplanted into a pure vermiculite substrate with a humidity of 55%, and when transplanting, the roots of the tissue-cultured seedlings should be stretched and Completely bury in the matrix, and compact the matrix around the tissue culture seedlings, and select tissue culture seedlings with similar growth heights to transplant into the same hole tray; after transplanting each hole tray, place them...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a seedling hardening method for iris tissue-cultured seedlings. The seedling hardening method comprises the following steps: cleaning roots of rooted iris tissue-cultured seedlings, carrying out sterilization, transplanting the iris tissue-cultured seedlings into a seedling-hardening matrix, and carrying out seedling hardening at a temperature of 10-13 DEG C and humidity of55%-85%, wherein the iris tissue-cultured seedlings are iris germanica tissue-cultured seedlings. According to the seedling hardening method provided by the invention, the temperature is limited at 10-13 DEG C, and meanwhile, the humidity is limited at 55%-85%, so that the seedling-hardening survival rate of the iris germanica tissue-cultured seedlings can be remarkably increased, and the cost islowered.

Description

technical field [0001] The invention relates to the technical field of agriculture, in particular to a method for hardening iris tissue cultured seedlings. Background technique [0002] German Iris (scientific name: Iris germanica L.) is a plant of the Iris family Iris, a perennial herb; it has thick rhizomes, the general flowering period is from May to June each year, and there are also two-season and multi-season flower varieties, such as The second flowering German iris can bloom twice a year. The flowering period is generally from mid-May to late June and early October to mid-November. It has a pleasant fragrance, and the flower shape is large and colorful. Red, orange, blue, purple, white, black and other colors, it is a precious ornamental flower; in addition, its roots contain a large amount of iris flavonoid glycosides with anti-inflammatory effect, which can be used to treat sore throat, Diseases such as wind pain and malaria; and active ingredients such as iris fl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G13/00A01G24/15A01G31/02
CPCA01G13/00A01G31/00A01G31/02A01G24/15Y02P60/21
Inventor 田菊铁英刘洋李瑞静
Owner INNER MONGOLIA HESHENG ECOLOGICAL SILVICULTURE CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap