Rapid seedling hardening method for tissue culture seedlings of dahlia pinnata

A technique for tissue culture of seedlings and dahlias, applied in the field of plant reproduction, can solve the problems of unstable seedling survival rate, poor operability and repeatability, and uneven growth of seedlings, so as to shorten the transition time, have high repeatability, and overcome uneven growth. uneven effect

Inactive Publication Date: 2019-01-04
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the low survival rate of hardened seedlings, slow growth of hardened seedlings and uneven growth of seedlings in the existing technology of hardening seedlings of dahlia tissue culture seedlings, hardening depends to a large extent on the experience of seedling growers, and the survival rate of hardened seedlings is extremely low. Stability, poor operability and repeatability, etc., the present invention provides a method for rapid seedling hardening of dahlia tissue culture seedlings, in the case of irregular rooting of tissue culture seedlings, through a series of measures, the survival rate of tissue culture seedlings can be improved and the growth can be neat , the method is feasible, highly repeatable, and can quickly popularize fine dahlia varieties

Method used

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  • Rapid seedling hardening method for tissue culture seedlings of dahlia pinnata

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Effect test

Embodiment 1

[0026] Taking the dahlia variety 'Burney's Tower' as an example, the present invention will be further described.

[0027] (1) Seedling hardening greenhouse preparation:

[0028] size is 20m 2 The seedling hardening greenhouse is routinely installed with sunshade nets, heating equipment, humidification equipment and irrigation equipment, so that the temperature in the hardening greenhouse is controlled between 10~28°C, and the relative air humidity is between 60~100%;

[0029] (2) Transplanting of tissue culture rooted seedlings:

[0030] a. Substrate preparation: take leaf humus as the substrate, add 800 times liquid thiophanate-methyl solution to the substrate and stir to make the substrate water content 70wt%, then spread it into a tray with a specification of 540mm×280mm and 128 holes;

[0031] b. Take out the rooted plantlets of tissue culture directly from the bottle, wash the culture medium on the plantlets, soak the plantlets with 800 times liquid carbendazim or chlo...

Embodiment 2

[0040] Taking the dahlia variety 'Weipa' as an example, the present invention will be further described.

[0041] (1) Seedling hardening greenhouse preparation:

[0042] size is 20m 2 The seedling hardening greenhouse is routinely installed with sunshade nets, heating equipment, humidification equipment and irrigation equipment, so that the temperature in the hardening greenhouse is controlled between 10~28°C, and the relative air humidity is between 60~100%;

[0043] (2) Transplanting of tissue culture rooted seedlings:

[0044] a. Substrate preparation: take mulch as the substrate, add 800 times liquid thiophanate-methyl solution to the substrate and stir to make the substrate water content 60wt%, and then spread it into a tray with a specification of 540mm×280mm and 128 holes;

[0045] b. Take out the rooted plantlets of tissue culture directly from the bottle, wash the culture medium on the plantlets, soak the plantlets with 1000 times liquid carbendazim or chlorothaloni...

Embodiment 3

[0054] The dahlia variety 'Burnie's Tower' was used as the experimental material.

[0055] (1) Seedling hardening greenhouse preparation:

[0056] The seedling hardening greenhouse is routinely installed with sunshade nets, heating equipment, humidification equipment and irrigation equipment, so that the temperature in the hardening greenhouse is controlled between 10~28°C, and the relative air humidity is between 60~100%;

[0057] (2) Transplanting of tissue culture rooted seedlings:

[0058] a. Substrate preparation: take leaf humus as the substrate, add 800 times liquid thiophanate-methyl solution into the substrate and stir to make the substrate water content 65wt%, and then spread it into the plug tray;

[0059] b. Take out the rooted plantlets of tissue culture directly from the bottle, wash the culture medium on the plantlets, soak the plantlets with 900 times liquid carbendazim or chlorothalonil solution for 15 minutes;

[0060] c. If the root length of the tissue cu...

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Abstract

The invention discloses a rapid seedling hardening method for tissue culture seedlings of dahlia pinnata, which comprises the steps of seedling hardening greenhouse preparation, transplantation of tissue culture rooting seedlings, environmental control after transplantation, fertilization management and disease and insect pest control, etc. It only takes 45 days for seedlings to grow out from a bottle to seedlings of 5 cm or above in plant height, compared with the prior art, the rapid seedling hardening method has the advantages that the seedling hardening time is shortened by 15 days or above, the transition time of the tissue culture seedlings of dahlia pinnata is greatly shortened, and the seedling hardening survival rate is high, and the survival rate is up to 95% or above, which is 5-7 times higher than that of the prior art, the hardened seedlings grow neatly, and the plant is robust. The invention provides a standardized seedling hardening technical scheme, which can be operated accurately, has high repeatability, is suitable for large-scale industrialized and standardized seedling hardening, and overcomes the defects that the prior art largely depends on the seedling-raising experience of the seedling-raiser and the survival rate is extremely unstable.

Description

technical field [0001] The invention relates to a method for rapid hardening of dahlia tissue cultured seedlings, which belongs to the technical field of plant propagation. Background technique [0002] Dahlia (Dahlia pinnata Cav.) is a perennial bulbous flower belonging to the genus Dahlia in the Asteraceae family. Native to Mexico, Guatemala and Colombia in Central America, it is now widely cultivated all over the world. Dahlias are rich in color and have various flower shapes, which can be compared with the peony, the king of flowers in national beauty and fragrance. Moreover, they bloom continuously from summer to autumn, so they are very popular. As one of the famous flowers in the world, dahlia not only has high ornamental value, but also contains "inulin" in its root, which has similar effects to glucose in medicine, and can be used as medicine, which has the effect of cooling, detoxifying and reducing swelling . Therefore, dahlias are not only admirable, but also ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/60A01G24/28
CPCA01G22/60A01G24/28
Inventor 段青王继华杜文文马璐琳王祥宁崔光芬贾文杰
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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