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A method for aseptic germination and seedling growth of staghorn fern platycerium wallichii Hook. spores

A technology for spore germination and staghorn fern, applied in the field of plant tissue culture, can solve the problems of low rooting rate, low reproduction efficiency, long seedling reproduction cycle, etc., and achieve the effects of short seedling time, high reproduction efficiency, and reduced culture cost.

Active Publication Date: 2021-05-04
CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the above documents have reported the tissue culture technology of staghorn fern, it is only limited to the young true leaves as explants, and there are low reproductive efficiency (GGB induction rate 50%), long seedling propagation cycle (more than 8 months), rooting Low rate (less than 94%) and other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Selection and disinfection of explants: Select the healthy mother plant of Staghorn fern difidoides as the object of explants, cut the leaves with mature spores, rinse them with tap water first, and then cut the leaves into pieces with a size of 5.0×2.0cm. Put it into an ultrasonic cleaner filled with sterile water and shake it for 30 minutes, take it out and put it in a sterile container, put it on an ultra-clean workbench, blot up the water with a sterile paper towel, scrape off the spores with a sharp knife, and set aside.

[0037] Spore germination: use tweezers to pick up the spores and spread them evenly on the surface of the spore germination medium for cultivation; the spores are seeded and cultured for 10 days, and gradually turn from brown to green, and expand and grow, and 28 days after inoculation, they germinate into green spheroids (GGB) (the germination rate is as high as 100.0%), and then GGB proliferated in large quantities. After 30 days of inoculation,...

Embodiment 2

[0042] Selection and disinfection of explants: Select a healthy mother plant of staghorn fern as the object of explants, cut out the leaves with mature spores, rinse them with tap water first, then cut the leaves into pieces with a size of 5.0×2.0cm, put them in Vibrate and clean with an ultrasonic cleaner filled with sterile water for 35 minutes, take it out and put it in a sterile container, on the ultra-clean workbench, dry the water with a sterile paper towel, scrape off the spores with a sharp knife, and set aside.

[0043] Spore germination: use tweezers to pick up the spores and spread them evenly on the surface of the spore germination medium for cultivation; the spores are seeded and cultured for 12 days, and gradually turn from brown to green, and expand and grow, and germinate into green spheroids (GGB) after inoculation for 30 days (the germination rate is as high as 100.0%), and then GGB proliferated in large quantities. After 35 days of inoculation, the bottom of ...

Embodiment 3

[0048] Selection and disinfection of explants: Select the healthy mother plant of Staghorn fern difidoides as the object of explants, cut the leaves with mature spores, rinse them with tap water first, and then cut the leaves into pieces with a size of 5.0×2.0cm. Put it into an ultrasonic cleaner filled with sterile water and shake it for 40 minutes, take it out and put it in a sterile container, put it on an ultra-clean workbench, blot up the water with a sterile paper towel, scrape off the spores with a sharp knife, and set aside.

[0049] Spore germination: use tweezers to pick up the spores and spread them evenly on the surface of the spore germination medium for cultivation; the spores are seeded and cultivated for 15 days, and gradually turn from brown to green, and expand and grow, and germinate into green spheroids (GGB) after 35 days of inoculation (the germination rate is as high as 100.0%), and then GGB proliferated in large quantities. After inoculation for 40 days,...

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Abstract

The invention provides a staghorn fern Platycerium wallichii The Hook. spore aseptic germination and seedling growth method uses staghorn fern leaves with mature spores as the explant material, designs special medium formulas for each culture stage, and achieves the purpose of rapid propagation through spore-GGB-buds, including the following steps: Selection and disinfection of explants, spore germination, GGB differentiation culture, rooting culture and transplanting of test-tube plantlets. The staghorn fern seedlings can be obtained in only 178d to 210d by adopting the method of the present invention; the spore germination of the present invention is carried out synchronously with the GGB multiplication, which simplifies the cultivation process and reduces the cultivation cost, and has a high spore germination rate, a high GGB multiplication rate, and a seedling The time is short, that is, the reproductive efficiency is high, and then the efficiency of the whole seedling cultivation is improved; in addition, the test-tube seedlings cultivated by the present invention are robust and have well-developed root systems, and through domestication and hardening, their environmental adaptability is strong, thereby improving the seedling transplantation. Planting survival rate, thereby realizing the purpose of rapid breeding of staghorn fern seedlings.

Description

【Technical field】 [0001] The invention belongs to the technical field of plant tissue culture, in particular to a staghorn fern Platycerium wallichii Hook. Spore aseptic germination seedling method. 【Background technique】 [0002] staghorn fern ( Platycerium wallichii Hook.) is a perennial evergreen herbaceous plant of the staghorn fern family Staghorn fern. It is native to Nabangba, Yingjiang County, southwest Yunnan, China, in mountain rainforests at an altitude of 210-950 meters, and is also distributed in Myanmar, northeastern India, and Thailand. This species has been included in the national second-class protected plants. Its unique plant shape and graceful posture make it the most peculiar type of fern among the ornamental ferns. There are two types of leaves, which are divided into basal peltate leaves and spore leaves. The basal peltate leaves are also called sterile leaves or vegetative leaves, which are round, oval or fan-shaped, and are attached to the rh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 叶秀仙陈艺荃方能炎林兵吴建设
Owner CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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