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Method for rapid propagation of pinellia ternate by tissue culture

A technology of tissue culture and Pinellia species, applied in the field of rapid propagation of Pinellia japonicus, can solve the problems of non-value-added coefficient, value-added effect data description, and the reproduction coefficient needs to be improved. The effect of increasing and multiplying quickly

Inactive Publication Date: 2019-05-17
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reproduction coefficient still needs to be improved, and there is no data explanation on the value-added coefficient and value-added effect

Method used

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  • Method for rapid propagation of pinellia ternate by tissue culture
  • Method for rapid propagation of pinellia ternate by tissue culture
  • Method for rapid propagation of pinellia ternate by tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Seed pretreatment: From June to July or October to November every year, collect mature Vitex pinellia seeds, remove the exocarp, and bathe in 35-degree water for 3-4 days.

[0042](2) Germination: On the ultra-clean workbench, take a sterile petri dish with two layers of sterile filter paper, moisten it with sterile water, put the sterilized seeds on the petri dish, and the seed disinfection method is 0.1 Sterilize with % potassium permanganate for 25 minutes, then 75% ethanol for 5 minutes, and finally 0.1% mercury liter for 10 minutes, rinse with sterile water for 4-5 times, each time for about 3 minutes, avoid light, accelerate germination at 25°C, and wait for the hypocotyl length to be 2- At 3 cm, stem segments were taken for callus induction.

[0043] (3) Callus induction: when the length of the hypocotyl is about 1.5cm, the hypocotyl is cut into small sections of about 0.6cm, and transferred to a solid medium.

[0044] Medium formula: MS+2,4-D0.5mg / L+6-BA0.5...

Embodiment 2

[0051] This embodiment is different in that: callus differentiation medium: MS+TDZ 0.5mg / L+agar 0.7mg / L+sucrose 30g / L, pH5.8; other operating steps are the same as in Example 1 .

Embodiment 3

[0053] the embodiment

[0054] Induction medium: MS+2,4-D 0.4mg / L+6-BA 0.4mg / L+NAA 0.1mg / L+agar 0.7mg / L+sucrose 30g / L, pH5.8;

[0055] Callus proliferation medium: MS+2,4-D 0.4mg / L+6-BA 0.8mg / L+NAA 0.1mg / L+glucose 30g / L, pH5.8;

[0056] Callus differentiation medium: MS+2,4-D 0.4mg / L+6-BA 0.8mg / L+NAA 0.1mg / L+agar 0.7mg / L+sucrose 30g / L, pH5.8;

[0057] Liquid accelerated medium: MS+2,4-D 0.4mg / L+6-BA 0.8mg / L+NAA 0.1mg / L+glucose 30g / L, pH5.8;

[0058] Solid strong seedling medium: MS+2,4-D 0.4mg / L+6-BA 0.8mg / L+NAA 0.1mg / L+glucose 30g / L, pH5.8;

[0059] Other operation steps are all identical with described embodiment 1.

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Abstract

The invention provides a method for rapid propagation of pinellia ternate by tissue culture. The method comprises the steps as follows: (1) preparation of culture media; (2) selection of explants; (3)obtaining of callus by induction; (4) multiplication culture of callus; (5) differentiation culture of the callus; (6) acceleration culture; (7) strong seedling culture; (8) acclimatization and transplantation. By use of the special culture media and liquid culture of the callus for 10 d, the volume and weight are increased by 300%, and the quality of the callus is better; when green bud points appear in solid culture media, the callus is transferred to liquid culture media again, the callus differentiates after 10 d of culture, a large number of root systems and bud points appear, and the volume and weight are obviously increased; then, the callus is transferred to the solid culture media, root systems continuously grow, the bud points continuously develop to form a large number of leaves, complete pinellia ternate plants can be formed after 15 d, the propagation coefficient reaches 20 or above, the rooting rate reaches 95% or above, and the transplantation survival rate is 99% or above.

Description

technical field [0001] The invention relates to the technical field of asexual propagation of seedlings, in particular to a rapid propagation method of Jingpinia tissue culture. Background technique [0002] Pinellia [Pinellia ternate (Thunb.) Breit.] belongs to the genus Pinellia of the family Araceae, and its dried tubers are used as medicine. It is an important traditional Chinese medicinal material. Element. It has the functions of lowering adverse flow and antiemetic, drying dampness and resolving phlegm, eliminating mass and resolving stagnation, etc. The ancient "Shenlong Materia Medica" called Pinellia Shoutian and Shuiyu. It is a perennial herb, mostly excavated in summer and autumn. Its tubers are spherical or oblate, and it likes sandy soil. The exact bulk of traditional Chinese medicinal materials. The original Jingzhou Youjiangling and Qianjiang are rich in quality and are commonly known as "Jingbanxia". Because of its excellent quality and excellent efficac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张建赵振军武彦芳曹运梅
Owner YANGTZE UNIVERSITY
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