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A method for cultivating seedlings of clematis protocorm imitating wild

A technology for cultivating seedlings and protocorms, which is applied in the field of imitating wild-cultured seedlings of A. aureus protocorms, can solve the problems of plant growth regulator residues, slow rooting process, and high production costs, and achieve the purpose of alleviating the contradiction between supply and demand and shortening the seedling time. Short, expensive to produce effects

Active Publication Date: 2020-06-26
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the plant tissue culture technology of clematis has broken through the bottleneck and has developed better, there are still some problems such as slow rooting process, long propagation cycle, high variability, low uniformity, low survival rate, high production cost and plant defects. How to solve the problems existing in the current clematis tissue culture seedlings, such as growth regulator residues, will be the key to solving the actual production problems of clematis

Method used

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  • A method for cultivating seedlings of clematis protocorm imitating wild

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Effect test

Embodiment 1

[0022] A method for cultivating seedlings of clematis protocorm imitating the wild, comprising the following steps:

[0023] (1) Aseptic sowing of mature capsules of Clematis clematis: Pick the mature capsules (75 days to 90 days after pollination) on a sunny day, sterilize them with 75% ethanol for 30 s on the ultra-clean workbench, and sterilize them with sterile water. Rinse once, blot the water on the surface of the capsule with sterile paper; then use 0.1% mercury chloride (HgCl 2 ) sterilized for 10 min, rinsed with sterile water for 5 times, and blotted the water on the surface of the capsule with sterile paper; cut one end of the capsule crosswise, sowed the seeds on the primary culture medium, and cultivated in the culture room for 5 days in the dark. After light culture, the seeds began to absorb water and become round and green in about 20 days; they began to germinate and form protocorms in about 45 days, and the protocorms were small, and the seed germination rate...

Embodiment 2

[0032] A method for cultivating seedlings of clematis protocorm imitating the wild, comprising the following steps:

[0033] (1) Aseptic sowing of mature capsules of Clematis clematis: Pick the mature capsules (75 days to 90 days after pollination) on a sunny day, sterilize them with 75% ethanol for 30 s on the ultra-clean workbench, and sterilize them with sterile water. Rinse once, blot the water on the surface of the capsule with sterile paper; then use 0.1% mercury chloride (HgCl 2 ) sterilized for 10 min, rinsed with sterile water for 5 times, and dried the surface moisture of the capsules with sterile paper; cut one end of the capsules crosswise, sow the seeds on the primary medium, and cultivate them in the cultivation room. After 5 days of dark cultivation, Carry out light cultivation, after 15 days, the seeds begin to absorb water and become round and green; 30 days, they begin to germinate to form protocorms, the protocorms are larger in size, and the seed germinatio...

Embodiment 3

[0042] A method for cultivating seedlings of clematis protocorm imitating the wild, comprising the following steps:

[0043] (1) Aseptic sowing of mature capsules of Clematis clematis: Pick the mature capsules (75 days to 90 days after pollination) on a sunny day, sterilize them with 75% ethanol for 30 s on the ultra-clean workbench, and sterilize them with sterile water. Rinse once, blot the water on the surface of the capsule with sterile paper; then use 0.1% mercury chloride (HgCl 2 ) sterilized for 10 min, rinsed with sterile water for 5 times, and dried the surface moisture of the capsules with sterile paper; cut one end of the capsules crosswise, sow the seeds on the primary medium, and cultivate them in the cultivation room. After 5 days of dark cultivation, After light culture, the seeds absorb water and become round and green after 17 days; the seeds germinate to form protocorms in about 35 days, the protocorms are larger, and the seed germination rate is 88.5%;

[0...

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Abstract

The invention discloses a method for imitating wild cultivation of seedlings via Anoectochilus roxburghii protocorms, which comprises: sterilizing mature capsules of Anoectochilus roxburghii as a material; slitting the capsules; sowing seeds to a culture medium; after the seeds germinate to form protocorms, carrying out propagation culture; directly broadcasting the protocorms into a field humus-rich forest land; building a small arch shed with film; allowing protocorms to grow roots and buds in the small arch shed with certain humidity and to absorb nutrients in nature; removing the film whenseedlings grow 2-3 leaves so that imitating wild propagation of seedlings via Anoectochilus roxburghii protocorms is achieved. The method has the advantages that the growth time of Anoectochilus roxburghii seedlings is short, the survival rate is high, the regularity is good, no variation occurs, no plant growth regulator residue exists during picking, the plant stress resistance is good, the growth is fast, the yield is high, the actual problem in the production of Anoectochilus roxburghii at present is solved, the contradiction between supply and demand of Anoectochilus roxburghii can be effectively relieved, and Anoectochilus roxburghii seedlings are high in yield and quality, the benefit is good, and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of cultivation of traditional Chinese medicinal materials, and in particular relates to a method for cultivating seedlings of protocorms of A. Background technique [0002] Clematis ( Anoectochilus roxburghii (Wall.) Lindl.) is a plant of the genus Orchidaceae. It is a traditional precious medicinal material in my country. The whole plant is used for medicine. It contains polysaccharides, flavonoids, alkaloids, trace elements and other ingredients. It has the effect of lowering blood sugar, and has good curative effect on diabetes, hematuria, anti-tumor and various inflammations. Clematis clematis is known as the "King of Medicine" among the people, and it is often used as a high-end food material for soup and nourishment in Fujian, Taiwan, Guangdong and other places. [0003] As people's living standards continue to improve and they pay more attention to health and health, the demand for medicinal and edi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G22/00A01G24/20A01G24/10
CPCA01G22/00A01G24/10A01G24/20A01H4/00A01H4/001
Inventor 石云平许娟廉永铭邱展鸿苏祖祥韦绍龙
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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