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A method for direct regeneration and in vitro rooting of Qizhu hypocotyls

A technology of exogenous root and hypocotyl, applied in the field of plant biology, can solve the problems of low multiplication coefficient and low seedling rate, and achieve the effects of simplifying reproduction steps, high seedling rate and high regeneration efficiency.

Active Publication Date: 2022-05-03
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the traditional Qishu seedling raising technology, this technology can speed up the artificial propagation speed of Qishu, but according to the existing research results, its multiplication coefficient is low and the seedling rate is also low in the seedling hardening process.

Method used

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  • A method for direct regeneration and in vitro rooting of Qizhu hypocotyls
  • A method for direct regeneration and in vitro rooting of Qizhu hypocotyls
  • A method for direct regeneration and in vitro rooting of Qizhu hypocotyls

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A method for directly regenerating hypocotyls and rooting in vitro, specifically comprising the following steps:

[0052] (1) Place the hypocotyls of the sterile seedlings of Qizhu that have been germinated for 3 weeks in a centrifuge tube filled with sterile water, and perform ultrasonic treatment for 60 seconds in an ultrasonic instrument (600W), and then place them in a sterile operating table. Cut into 0.3cm sections and set aside;

[0053] (2) The hypocotyl sections in step (1) were inoculated horizontally in the adventitious bud induction medium ( figure 1 ), at a temperature of 22±2°C, the light source is LED white light, and the light intensity is 50μmol·m -2 ·s -1 , the light time is 16h, and the relative humidity is 50-60% in a constant temperature culture room to carry out the induction and subculture of adventitious buds. After 2 weeks of light culture, adventitious bud clusters were induced at the hypocotyl incision ( figure 2 ); after continuing to cu...

Embodiment 2

[0058] In this example, the effect of ultrasonic treatment time on the induction of adventitious buds in Qizhu hypocotyls was tested.

[0059] The hypocotyls from the sterile seedlings of Qizhu that had germinated for 3 weeks were placed in a centrifuge tube filled with sterile water and subjected to ultrasonic treatment for different times (0, 30, 60, 120 and 180s) in a sonicator (600W) , and then cut into 0.3cm sections in a sterile operating table, inoculated horizontally in the adventitious bud induction medium at a temperature of 22±2°C, the light source is LED white light, and the light intensity is 50μmol·m -2 ·s -1 , the light time is 16h, and the relative humidity is 50-60% in a constant temperature culture room to carry out the induction culture of adventitious buds. After 3 weeks of light culture, the induction rate of adventitious buds and the average number of adventitious buds produced by each explant were counted. The results of the study (Table 1) show that u...

Embodiment 3

[0064] In this example, the effects of different light sources and light ratios on the elongation of Qishu hypocotyl adventitious buds were tested.

[0065] The elongated Qizhu tissue culture buds in Example 1 were isolated and inoculated in the elongation medium to carry out elongation culture of adventitious buds under different light quality conditions. The light quality was cultivated with white light, red light, blue light, red-blue composite light (1:1) and red-blue composite light (3:1) respectively. After 3 weeks of light cultivation, the elongation of adventitious buds was counted.

[0066] The research results show (Table 2) that light quality and ratio play an important role in the elongation process of Qishu adventitious buds. Among the different light quality species tested, when LED red:LED blue=3:1, Qishu adventitious buds The fastest growth rate, the best elongation effect, the average height of 4.4cm.

[0067] Table 2 Effect of LED light quality on elongatio...

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Abstract

The invention discloses a method for directly regenerating hypocotyls and rooting in vitro, and relates to the field of plant biotechnology. The invention includes the following steps: (1) pretreatment of explants; (2) induction and subculture of adventitious buds Proliferation culture; (3) elongation culture of adventitious buds; (4) in vitro rooting treatment of elongated buds; (5) colonization and seedling cultivation of elongated buds. The beneficial effect of the present invention is that: the breeding process involved in the present invention has the advantages of high regeneration efficiency, simple operation, short seedling time, high seedling rate, etc., which lays the foundation for the large-scale breeding and variety improvement of later Qishu seedlings.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a method for direct regeneration of hypocotyls and rooting in vitro. Background technique [0002] Qishu is Qimen Atractylodes, a perennial herb of the Asteraceae Atractylodes family. It is one of the "three Qi" (Qishu, Qihongcha, Qisnake) famous and high-quality products in Huizhou. It is a rare medicinal material in Anhui and has important medicinal value , is the raw material medicine of many kinds of Chinese patent medicines. It has many functions such as benefiting the stomach, promoting dampness, stopping diarrhea, nourishing the mind, preventing miscarriage, eliminating fatigue and collapse, and is very effective in the rehabilitation of patients with jaundice, heart disease, gastric ulcer and ascites. , "Ten directions and nine arts". As an authentic medicinal material, Qishu is superior in quality and curative effect to the same medicinal materials in other regions, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G22/00A01G24/10A01G24/15
CPCA01H4/008A01H4/002A01G22/00A01G24/10A01G24/15
Inventor 侯金艳吴丽芳王萍王军
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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