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A kind of culture medium for inducing the differentiation of protocorm of dendrobium nobile

A protocorm differentiation and dendrobium purple technology, which is applied to plant cells, horticulture, botanical equipment and methods, etc., can solve problems that affect the popularization and application of dendrobium purple skin, long seedling time, and harm to health, and achieve leaf color Green, short seedling time, cost-saving effect

Inactive Publication Date: 2015-10-21
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But for many years, when Dendrobium purple-skinned Dendrobium is differentiated by protocorm, more hormones are added in the medium used, and the seedling time is longer, which seriously endangers people's health and affects the popularization and application of Dendrobium purple-skinned Dendrobium.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Weigh an appropriate amount of KNO 3 , NH 4 NO 3 、KH 2 PO 4 , MgSO 4 ·7H 2 O, CaCl 2 2H 2 O, KI, H 3 BO 3 , MnSO 4 4H 2 O, ZnSO 4 ·7H 2 O, Na 2 MoO 4 2H 2 O, CuSO 4 ·5H 2 O, CoCl 2 ·6H 2 O, Na 2 EDTA, FeSO 4 ·7H 2 O, inositol, glycine, thiamine hydrochloride, pyridoxine hydrochloride, nicotinic acid, sucrose, agar, banana mash, potato mash and activated carbon were mixed to obtain a medium with a pH of 5.

[0016] And the content of each component in this culture medium is:

[0017] KNO 3 923mg / L, NH 4 NO 3 780mg / L, KH 2 PO 4 85mg / L, MgSO 4 ·7H 2 O200mg / L, CaCl 2 2H 2 O200mg / L, KI0.685mg / L, H 3 BO 3 7mg / L, MnSO 4 4H 2 O25mg / L, ZnSO 4 ·7H 2 O7.8mg / L, Na 2 MoO 4 2H 2 O0.23mg / L, CuSO 4 ·5H 2 O0.025mg / L, CoCl 2·6H 2 O0.025mg / L, Na 2 ·EDTA35mg / L, FeSO 4 ·7H 2 O28mg / L, inositol 100mg / L, glycine 1.8mg / L, thiamine hydrochloride 0.5mg / L, pyridoxine hydrochloride 0.5mg / L, niacin 0.5mg / L, sucrose 30000mg / L, agar 5000mg / L , Mashed ba...

Embodiment 2

[0019] Weigh an appropriate amount of KNO 3 , NH 4 NO 3 、KH 2 PO 4 , MgSO 4 ·7H 2 O, CaCl 2 2H 2 O, KI, H 3 BO 3 , MnSO 4 4H 2 O, ZnSO 4 ·7H 2 O, Na 2 MoO 4 2H 2 O, CuSO 4 ·5H 2 O, CoCl 2 ·6H 2 O, Na 2 EDTA, FeSO 4 ·7H 2 O, inositol, glycine, thiamine hydrochloride, pyridoxine hydrochloride, nicotinic acid, sucrose, agar, banana mash, potato mash and activated carbon were mixed to obtain a culture medium with a pH of 6.

[0020] And the content of each component in this culture medium is:

[0021] KNO 3 1450mg / L, NH 4 NO 3 495mg / L, KH 2 PO 4 50mg / L, MgSO 4 ·7H 2 O275mg / L, CaCl 2 2H 2 O330mg / L, KI0.83mg / L, H 3 BO 3 9.00mg / L, MnSO 4 4H 2 O34.5mg / L, ZnSO 4 ·7H 2 O12.8mg / L, Na 2 MoO 4 2H 2 O0.35mg / L, CuSO 4 ·5H 2 O0.035mg / L, CoCl 2 ·6H 2 O0.035mg / L, Na 2 ·EDTA55mg / L, FeSO 4 ·7H 2 O42mg / L, inositol 300mg / L, glycine 2.8mg / L, thiamine hydrochloride 0.7mg / L, pyridoxine hydrochloride 0.7mg / L, niacin 0.7mg / L, sucrose 55000mg / L, agar 8000mg...

Embodiment 3

[0023] Weigh an appropriate amount of KNO 3 , NH 4 NO 3 、KH 2 PO 4 , MgSO 4 ·7H 2 O, CaCl 2 2H 2 O, KI, H 3 BO 3 , MnSO 4 4H 2 O, ZnSO 4 ·7H 2 O, Na 2 MoO 4 2H 2 O, CuSO 4 ·5H 2 O, CoCl 2 ·6H 2 O, Na 2 EDTA, FeSO 4 ·7H 2 O, inositol, glycine, thiamine hydrochloride, pyridoxine hydrochloride, nicotinic acid, sucrose, agar, banana mash, potato mash and activated carbon were mixed to obtain a medium with a pH of 5.5.

[0024] And the content of each component in the medium is: KNO 3 550mg / L, NH 4 NO 3 1245mg / L, KH 2 PO 4 125mg / L, MgSO 4 ·7H 2 O120mg / L, CaCl 2 2H 2 O135mg / L, KI0.45mg / L, H 3 BO 3 3.75mg / L, MnSO 4 4H 2 O13.5mg / L, ZnSO 4 ·7H 2 O5.2mg / L, Na 2 MoO 4 2H 2 O0.15mg / L, CuSO 4 ·5H 2 O0.01mg / L, CoCl 2 ·6H 2 O0.01mg / L, Na 2 ·EDTA25mg / L, FeSO 4 ·7H 2 O18mg / L, inositol 50mg / L, glycine 1.2mg / L, thiamine hydrochloride 0.3mg / L, pyridoxine hydrochloride 0.3mg / L, niacin 0.3mg / L, sucrose 18000mg / L, agar 2500mg / L , Mashed banana 30000mg / ...

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Abstract

The invention relates to a culture medium capable of inducing differentiation of protocorm of dendrobium, and belongs to the technical field of plant tissue culture. The culture medium comprises following components: KNO3, NH4NO3, KH2PO4, MgSO4.7H2O, CaCl2.2H2O, KI, H3BO3, MnSO4.4H2O, ZnSO4.7H2O, Na2MoO4.2H2O, CuSO4.5H2O, CoCl2.6H2O, Na2.EDTA, FeSO4.7H2O, inositol, glycine, thiamine hydrochloride, pyridoxine hydrochloride, niacin, cane sugar, agar, mashed banana, mashed potato, and activated carbon. The culture medium has the beneficial effects of containing no hormone and shortening the seedling growing time.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a culture medium for inducing the differentiation of protocorms of Dendrobium officinale. Background technique [0002] Dendrobium devonianum Paxt is a kind of Dendrobium devonianum Paxt, represented by Dendrobium dendrobium. It is called Dendrobium devonianum Paxt because of its purple stem. "Shen Nong's Materia Medica" records: "Dendrobium, a forest orchid. It tastes sweet, flat, and non-toxic. In the main injury, it eliminates numbness, nourishes the five internal organs, thickens the stomach, lightens the body and prolongs life." "Compendium of Materia Medica" claims that Dendrobium has the effect of "strengthening yin and benefiting essence, thickening the stomach, nourishing internal deficiencies, lightening body and prolonging life". [0003] The artificial cultivation of Dendrobium dendrobii began in the 1990s and formed scattered small-scale pla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 李旦王俊陈远书潘瑶
Owner SOUTHWEST FORESTRY UNIVERSITY
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