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Cultivating method of oncorhynchus mykiss walbaum gynoecial hybrid offspring seed

A breeding method and technology of golden trout, which is applied in the field of breeding all-female hybrid fish of golden trout, can solve problems such as no reports, and achieve the effects of reducing breeding costs, maintaining the rate of golden trout, and shortening the breeding cycle

Inactive Publication Date: 2014-03-05
BEIJING FISHERIES RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the preparation technology of all-female hybrid fry of golden trout

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 All-female hybrid seedling cultivation method (1)

[0027] 1. Dissolve 17α methyl testosterone with alcohol with a purity of 65%. The mass volume ratio of 17α methyl testosterone and alcohol is 1mg: 2ml. After the dissolved methyl testosterone solution is diluted 10 times with water, spray it evenly on the On the broodstock feed, the content of 17α methyltestosterone in the feed was 60mg / kg. Stir repeatedly to make it evenly mixed, and dry it in a ventilated place for later use.

[0028] 2. Select sexually mature female steelhead trout broodstock with a size of 2.6kg and above 3 years old and put them into a separate breeding pond, and feed the broodstock feed that has been prepared in step 1 containing 17α methyl testosterone, and raise them according to the conventional method Methods Feed for 100 days, then switch to normal parent fish feed without 17α methyltestosterone and continue to feed for 4 months. Among the female steelhead trout induced by 17α...

Embodiment 2

[0032]Embodiment 2 All-female hybrid seedling cultivation method (2)

[0033] 1. Dissolve 17α-methyltestosterone in alcohol with a purity of 70%, and the mass-volume ratio of 17α-methyltestosterone to alcohol is 1mg: 1ml. After the methyltestosterone solution after dissolving is diluted 10 times with clear water, spray on the parent fish feed evenly with a watering can, make the content of 17α methyltestosterone in the feed be 20mg / kg. Stir repeatedly to make it evenly mixed, and dry it in a ventilated place for later use.

[0034] 2. Select sexually mature female steelhead trout broodstock of 2.5kg and above age 3 and put them into a separate breeding pond, and feed them with the feed that has been formulated in step 1 containing 17α methyl testosterone, and follow the conventional feeding method Feed for 120 days, then switch to normal parent fish feed without 17α methyltestosterone and continue to feed for 5 months. Among the female steelhead trout induced by 17α methylte...

Embodiment 3

[0039] Embodiment 3 All-female hybrid seedling cultivation method (3)

[0040] 1. Dissolve 17α-methyltestosterone with alcohol with a purity of 60%. The mass-volume ratio of 17α-methyltestosterone to alcohol is 1mg: 2ml of the dissolved methyltestosterone solution is diluted 10 times with water and sprayed evenly on broodstock feed with a watering can. Above, make the content of 17α methyltestosterone in the feed be 40mg / kg. Stir repeatedly to make it evenly mixed, and dry it in a ventilated place for later use.

[0041] 2. Select sexually mature female steelhead trout broodstock of 2.55kg and above age 3 and put them into a separate breeding pond, and feed them with the feed that has been prepared in step 1 containing 17α methyl testosterone, and follow the conventional feeding method Feed for 90 days, then switch to normal parent fish feed without 17α methyltestosterone and continue to feed for 5 months. Among the female steelhead trout induced by 17α methyltestosterone an...

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Abstract

The invention provides a cultivating method of an oncorhynchus mykiss walbaum gynoecial hybrid offspring seed. The method comprises the following steps of: using male sex hormone to induce female oncorhynchus mykiss parent fishes in a sexual maturity stage to obtain oncorhynchus mykiss false milters with male functions; and fertilizing oncorhynchus mykiss walbaum eggs and oncorhynchus mykiss false milter sperms, and incubating to obtain gynoecial hybrid oncorhynchus mykiss walbaum offspring seeds. The eyeing rate of fertilized eggs in an incubating period is more than 83 percent, the offspring seed incubating rate reaches more than 90 percent, through test, the female ratio of the obtained seeds is 100 percent, the oncorhynchus mykiss walbaum ( golden yellow) rate of the offspring seeds reaches more than 91 percent, the weight gain rate of the offspring seed is more than 114 percent after 30-day cultivation, and the survival rate reaches more than 98 percent. Compared with an offspring seed produced by mating of a purebred oncorhynchus mykiss walbaum, according to the cultivating method of the oncorhynchus mykiss walbaum gynoecial hybrid offspring seed provided by the invention, the oncorhynchus mykiss walbaum rate remains unchanged, the oncorhynchus mykiss walbaum gynoecial hybrid offspring seeds with double advantages of hybridization and gynoecious are obtained, and better economic benefit and market value are achieved.

Description

technical field [0001] The invention relates to the technical field of aquatic organism breeding, in particular to a method for cultivating golden trout all-female hybrid fry. Background technique [0002] Salmon and trout are currently one of the main farmed fishes in the world. Under natural conditions, they mostly grow in low-temperature hot spring water or clear streams where the water temperature does not exceed 20°C, the water quality is oligotrophic, and there is no chemical or organic pollution. , so it is also called cold water fish. Because salmonids have very high requirements on the quality of living water, they cannot survive with slight pollution. Therefore, salmon and trout are clean and free of any pollution. They are veritable pollution-free green food, and they are safe to eat. In addition, salmon trout is a high-protein, high-fat, low-cholesterol (very little cholesterol) fish. Compared with other fish, salmon and trout contain glycine, which is lacking ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 徐绍刚李文通田照辉杨晓飞
Owner BEIJING FISHERIES RES INST
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