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A kind of cultivation method of passion fruit seedling

A cultivation method, passion fruit technology, applied in fruit tree cultivation, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of weak sprouting ability and limited yield

Active Publication Date: 2021-08-13
XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] Patent (publication number CN201811584620.9) "A Seedling Cultivation Method for Enhancing the Resistance of Passion Fruit Plants to Stem Rot Passiflora safflower as rootstock, grafted with branches of different high-quality passion fruit varieties as scions to enhance the ability of passion fruit seedlings to resist stem and base rot, but it was found that the grafted seedlings using this rootstock had lower branch germination ability in field planting. Weak, limited output

Method used

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  • A kind of cultivation method of passion fruit seedling
  • A kind of cultivation method of passion fruit seedling
  • A kind of cultivation method of passion fruit seedling

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Experimental program
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Effect test

Embodiment 1

[0053] A kind of cultivation method of passion fruit stock of embodiment 1,

[0054]One, the cultivation of the hybrid progeny of rootstock fine-toothed green fruit adopts the conventional pollination method to carry out pollination, including the following steps:

[0055] (1) Selection and pretreatment of the female parent: On September 20, 2019, select 10 buds of healthy and disease-free Passiflora serrata that are about to open, use tweezers to open the petals and remove all their pollen, and use an impermeable Kraft paper bag to secure it and write the label;

[0056] (2) Selection and pretreatment of male parents: From 8:00 am to 10:00 am on September 21, 2019, select normal open,

[0057] The flowers of the healthy and disease-free green fruit passionflower, use a clean brush to sweep its mature pollen into a clean disposable petri dish;

[0058] (3) Pollination: From 8:00 to 10:00 in the morning on September 21, 2019, use a clean brush to smear the pollen of Passiflor...

Embodiment 2

[0070] Embodiment 2 A kind of cultivation method of passion fruit seedling

[0071] One, the cultivation of rootstock green fruit fine-toothed hybrid progeny adopts the conventional pollination method to carry out pollination, including the following steps:

[0072] (1) Selection and pretreatment of the female parent: On October 8, 2019, select 15 buds of healthy and disease-free Passiflora japonica that are about to open, open the petals with tweezers and remove all their pollen, and use a water-tight Kraft paper bag to secure it and write the label;

[0073] (2) Selection and pretreatment of the male parent: From 8:00 to 10:00 on October 9, 2019, select the flowers of Passiflora serrata that are normally open, healthy and disease-free, and use a clean brush to remove the mature pollen Sweep and drop into a clean disposable petri dish;

[0074] (3) Pollination: From 8:00 to 10:00 am on October 9, 2019, use a clean brush to smear the pollen of Passiflora japonica on the stig...

Embodiment 3

[0086] Embodiment 3 rootstock resistance experiment comparison

[0087] The resistance of rootstocks was compared by comparing the in vivo inoculation experiment of Passiflora serrata, the hybrid progeny of the green fruit and the hybrid progeny of the fine-toothed green fruit, and the control pictures of the stems of the experimental seedlings.

[0088] The inoculation method was used to verify the ability of the stem rot resistance of the hybrid progenies of the green fruit. Inoculate Fusarium solani (F. solani) on the PDA medium, place it in a constant temperature incubator at 28°C and incubate in the dark for 3-5 days. When the colony is half full of the medium, use a sterile puncher The colony-growing culture medium was beaten into small cakes with a diameter of 60 mm. Select 20 strains of fine-toothed green fruit hybrids whose seedling age is 4-6 months and have the same growth potential, and cut 0.5cm × 0.2cm wounds with a blade at the base of their stems, and place fr...

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Abstract

The invention belongs to the field of cultivation and application of high-quality seedlings, and relates to a method for cultivating passion fruit seedlings. The present invention creatively selects the hybrid progeny F1 obtained by crossing Passiflora serrata with strong resistance and Passiflora viridis as the rootstock, selects young passion fruit branches with leaf buds as the scion, and uses the grafting method to cultivate the hybrid offspring F1. The seedlings have strong ability to resist stem base rot, and the branches of the seedlings are luxuriant, thereby reducing the frequency of seedling renewal and planting in the process of planting passion fruit varieties, reducing the cost of cultivation management, prolonging the life of passion fruit plants, and increasing the harvesting period of fruits. It is of great significance to promote the large-scale planting of passion fruit. It is simple to operate, low in cost, suitable for large-scale production in agriculture, and has important enlightenment for the cultivation of high-quality seedlings of other plants of the Passiflora genus.

Description

technical field [0001] The technical invention belongs to the field of cultivation and application of high-quality seedlings, and in particular relates to a method for cultivating passion fruit seedlings. Background technique [0002] At present, the edible varieties of passionflower are mainly 6 types: purple fruit passionflower (Passiflora edulis Sims), yellow fruit passionflower (P.edulis Sim.F.flavicarpa Deg.), large fruit passionflower (P. .quadrangularisL.), banana passionflower (P.mollissima (Kunth) L.H.Bailey), camphor leaf passionflower (P.laurifolia L.), sweet fruit passionflower (P.ligularis Juss); The horticultural varieties of the species, the yellow fruit species, and the series of varieties that are crossed between the varieties are called passion fruit. Passion fruit is a tropical perennial herbaceous evergreen to woody climbing vine belonging to the Passiflora family Passiflora. Its origin is located in Australia and Brazil, and it is now widely planted in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/05A01G2/30A01H1/02
CPCA01H1/02A01G2/30A01G22/05
Inventor 郝春卉吴福川
Owner XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI
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