A kind of cultivation method of passion fruit seedling
A cultivation method, passion fruit technology, applied in fruit tree cultivation, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of weak sprouting ability and limited yield
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Embodiment 1
[0053] A kind of cultivation method of passion fruit stock of embodiment 1,
[0054]One, the cultivation of the hybrid progeny of rootstock fine-toothed green fruit adopts the conventional pollination method to carry out pollination, including the following steps:
[0055] (1) Selection and pretreatment of the female parent: On September 20, 2019, select 10 buds of healthy and disease-free Passiflora serrata that are about to open, use tweezers to open the petals and remove all their pollen, and use an impermeable Kraft paper bag to secure it and write the label;
[0056] (2) Selection and pretreatment of male parents: From 8:00 am to 10:00 am on September 21, 2019, select normal open,
[0057] The flowers of the healthy and disease-free green fruit passionflower, use a clean brush to sweep its mature pollen into a clean disposable petri dish;
[0058] (3) Pollination: From 8:00 to 10:00 in the morning on September 21, 2019, use a clean brush to smear the pollen of Passiflor...
Embodiment 2
[0070] Embodiment 2 A kind of cultivation method of passion fruit seedling
[0071] One, the cultivation of rootstock green fruit fine-toothed hybrid progeny adopts the conventional pollination method to carry out pollination, including the following steps:
[0072] (1) Selection and pretreatment of the female parent: On October 8, 2019, select 15 buds of healthy and disease-free Passiflora japonica that are about to open, open the petals with tweezers and remove all their pollen, and use a water-tight Kraft paper bag to secure it and write the label;
[0073] (2) Selection and pretreatment of the male parent: From 8:00 to 10:00 on October 9, 2019, select the flowers of Passiflora serrata that are normally open, healthy and disease-free, and use a clean brush to remove the mature pollen Sweep and drop into a clean disposable petri dish;
[0074] (3) Pollination: From 8:00 to 10:00 am on October 9, 2019, use a clean brush to smear the pollen of Passiflora japonica on the stig...
Embodiment 3
[0086] Embodiment 3 rootstock resistance experiment comparison
[0087] The resistance of rootstocks was compared by comparing the in vivo inoculation experiment of Passiflora serrata, the hybrid progeny of the green fruit and the hybrid progeny of the fine-toothed green fruit, and the control pictures of the stems of the experimental seedlings.
[0088] The inoculation method was used to verify the ability of the stem rot resistance of the hybrid progenies of the green fruit. Inoculate Fusarium solani (F. solani) on the PDA medium, place it in a constant temperature incubator at 28°C and incubate in the dark for 3-5 days. When the colony is half full of the medium, use a sterile puncher The colony-growing culture medium was beaten into small cakes with a diameter of 60 mm. Select 20 strains of fine-toothed green fruit hybrids whose seedling age is 4-6 months and have the same growth potential, and cut 0.5cm × 0.2cm wounds with a blade at the base of their stems, and place fr...
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