Method for cultivating passion fruit seedlings
A cultivation method and technology of passion fruit, which are applied in the directions of fruit tree cultivation, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of weak branch germination ability and limited yield, etc., and achieve short time required for seedlings and strong growth potential. , the effect of improving the resistance to stem base rot
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[0053]Example 1 A cultivation method of a passion fruit rootstock,
[0054]First, the cultivation of the ethocstock fine decorate hybrid agents, the pollination is carried out in conventional pollination, including the following steps:
[0055](1) Master's choice and premium: On September 20, 2019, 10 healthy and neuropathy Shuangfu lotus will open 10 flowers, open the petals and remove all pollen with tweezers. Kraft paper bags and write its labels;
[0056](2) The choice and pre-processing of the father: 8:10 in the morning of September 21, 2019, choose normal opening,
[0057]Healthy and non-ill-illgedible green chevilian lotus, use a clean brush to sweep their ripe pollen to clean disposal culture dishes;
[0058](3) Pollination: At 8:00 on September 21, 2019, the use of clean brush to apply the pollen of the green chest pastard on September 20th. bag;
[0059](4) After pollination: On September 25, 2019, the kraft paper bag is removed, of which 8 of them are swearing, and the sizes of Sichu Lotu...
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[0070]Example 2 Method for cultivating a variety of brevnel seedlings
[0071]First, the cultivation of the ethocstut green fruit is mixed, and the pollination is carried out in conventional pollination, including the following steps:
[0072](1) Master's choice and premium: On October 8, 2019, choose a healthy and non-ill-conditioned Green Chessian lotus is about 15, open the petals and remove all pollen with tweezers. Kraft paper bags and write its labels;
[0073](2) The choice and preparation of the father: At 8:00 am - 10 o'clock on October 9, 2019, choose normal open, healthy non-disease, Western Divine, using a clean brush to make its ripe pollen Sweep in a clean disposal culture dish;
[0074](3) Pollination: On October 9, 2019, 8:00, using a clean brush to apply the pollen of the green chevs in the stunt of the fine teeth treated on October 2nd, set bag;
[0075](4) After pollination: On September 25, 2019, the kraft bag is removed, 15 pollinated green chevs, the subsacks of the Sichu lot...
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[0086]Example 3 Roofing resistance experiment comparison
[0087]By comparing the fine teeth Passion, the green fruit is mixed with the descendants, the fine decay hybrid regenerative biopsy, and the experimental seedlings are compared to compare the resistance of rootstocks.
[0088]It is verified using a living body, and the anti-stem-based corrugation capability of the anti-decorative green fruit hybrid age is verified. Silipbene (F. Solani) was backedily on the PDA medium, and in a constant temperature incubator of 28 ° C, inverted dark culture for 3-5 days, when the bacteria was long, the sterile punch was used. Put the long colonies into a small fungus having a diameter of 60 mm. The selection of the seedling lesion was 4-6 months, and the raw gangfanese hybrid seeds were selected, and the wound of 0.5 cm × 0.2cm in its stem base was used, and 10 of the wounds of 10 strains were placed toward the wound. The fungus is fixed to the wound with a sealing film; the addition of 10 strains...
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