Method for cultivating passion fruit seedlings
A cultivation method and technology of passion fruit, which are applied in the directions of fruit tree cultivation, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of weak branch germination ability and limited yield, etc., and achieve short time required for seedlings and strong growth potential. , the effect of improving the resistance to stem base rot
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Embodiment 1
[0053] A kind of cultivation method of passion fruit stock of embodiment 1,
[0054] One, the cultivation of the hybrid progeny of rootstock fine-toothed green fruit adopts the conventional pollination method to carry out pollination, including the following steps:
[0055] (1) Selection and pretreatment of the female parent: On September 20, 2019, select 10 buds of healthy and disease-free Passiflora serrata that are about to open, use tweezers to open the petals and remove all their pollen, and use an impermeable Kraft paper bag to secure it and write the label;
[0056] (2) Selection and pretreatment of male parents: From 8:00 am to 10:00 am on September 21, 2019, select normal open,
[0057]For the flowers of healthy and disease-free green passionflower, use a clean brush to sweep its mature pollen into a clean disposable petri dish;
[0058] (3) Pollination: From 8:00 to 10:00 in the morning on September 21, 2019, use a clean brush to smear the pollen of Passiflora japo...
Embodiment 2
[0070] Embodiment 2 A kind of cultivation method of passion fruit seedling
[0071] One, the cultivation of rootstock green fruit fine-toothed hybrid progeny adopts the conventional pollination method to carry out pollination, including the following steps:
[0072] (1) Selection and pretreatment of the female parent: On October 8, 2019, select 15 buds of healthy and disease-free Passiflora japonica that are about to open, open the petals with tweezers and remove all their pollen, and use a water-tight Kraft paper bag to secure it and write the label;
[0073] (2) Selection and pretreatment of the male parent: at 8:00-10:00 am on October 9, 2019, select the flowers of Passiflora serrata that are normally open, healthy and disease-free, and use a clean brush to remove the mature pollen Sweep and drop into a clean disposable petri dish;
[0074] (3) Pollination: From 8:00 to 10:00 am on October 9, 2019, use a clean brush to smear the pollen of Passiflora japonica on the stigma...
Embodiment 3
[0086] Embodiment 3 rootstock resistance experiment comparison
[0087] The resistance of the rootstocks was compared by comparing the inoculation experiments of Passiflora serrata, the hybrid progenies of the green fruit and the hybrid progenies of the fine-toothed green fruit, and the control pictures of the stems of the experimental seedlings.
[0088] The inoculation method was used to verify the ability of the stem rot resistance of the hybrid progenies of the green fruit. Inoculate Fusarium solani (F.solani) on the PDA medium, place it in a constant temperature incubator at 28°C and incubate in the dark for 3-5 days, and when the colony is half full of the medium, use a sterile puncher The colony-growing culture medium was beaten into small cakes with a diameter of 60 mm. Select 20 strains of the fine-toothed green fruit hybrids whose seedling age is 4-6 months and have the same growth potential, draw a wound of 0.5 cm × 0.2 cm with a blade at the base of its stem, and ...
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